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Gene Expression

Inactive Chromatin Spreads from a Focus of Methylation

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Pages 7372-7379 | Received 07 Apr 1993, Accepted 30 Jun 1993, Published online: 31 Mar 2023

References

  • Adams, R., L. P. 1990. DNA methylation: the effect of minor bases on DNA-protein interactions. Biochem. J. 265:309–320.
  • Antequera, F., D. Macleod, and A. P. Bird. 1989. Specific protection of methylated CpGs in mammalian nuclei. Cell 58:509–517.
  • Bird, A.%% 1992. The essentials of DNA methylation. Cell 70:5–8.
  • Blondel, A., and J. Thillet. 1991. A fast and convenient way to produce single stranded DNA from a phagemid. Nucleic Acids Res. 19:181.
  • Boyes, J., and A. Bird. 1991. DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein. Cell 64:1123–1134.
  • Boyes, J., and A. Bird. 1992. Repression of genes by DNA methylation depends on CpG density and promoter strength: evidence for involvement of a methyl-CpG binding protein. EMBO J. 11:327–333.
  • Bryans, M., S. Kass, C. Seivwright, and R. L. P. Adams. 1992. Vector methylation inhibits transcription from the SV40 early promoter. FEBS Lett. 309:97–102.
  • Buschhausen, G., B. Wittig, M. Graessmann, and A. Graess-mann. 1987. Chromatin structure is required to block transcription of the methylated herpes simplex virus thymidine kinase gene. Proc. Natl. Acad. Sci. USA 84:1177–1181.
  • Busslinger, M., J. Hurst, and R. A. Flavell. 1983. DNA methylation and the regulation of globin gene expression. Cell 34:197–206.
  • Clark, D. J., and G. Felsenfeld. 1992. A nucleosome core is transferred out of the path of a transcribing polymerase. Cell 71:11–22.
  • Comb, M., and H. M. Goodman. 1990. CpG methylation inhibits proenkephalin gene expression and binding of the transcription factor AP-2. Nucleic Acids Res. 18:3975–3982.
  • Deobagkar, D. D., M. Liebler, M. Graessmann, and A. Graessmann. 1990. Hemimethylation of DNA prevents chromatin expression. Proc. Natl. Acad. Sci. USA 87:1691–1695.
  • Felsenfeld, G.%% 1992. Chromatin as an essential part of the transcriptional mechanism. Nature (London) 355:219–224.
  • Graessmann, M., and A. Graessmann. 1993. DNA methylation, chromatin structure and the regulation of gene expression, p. 404-424. In J. P. Jost and H. P. Saluz (ed.), DNA methylation: molecular biology and biological significance. Birkhäuser Verlag, Basel.
  • Graessmann, M., A. Graessmann, H. Wagner, E. Werner, and D. Simon. 1983. Complete DNA methylation does not prevent polyoma and simian virus 40 virus early gene expression. Proc. Natl. Acad. Sci. USA 80:6470–6474.
  • Hansen, R. S., N. A. Ellis, and S. M. Gartler. 1988. Demeth-ylation of specific sites in the 5′ region of the inactive X-linked human phosphoglycerate kinase gene correlates with the appearance of nuclease sensitivity and gene expression. Mol. Cell. Biol. 8:4692–4699.
  • Harrington, M. A., P. A. Jones, M. Imagawa, and M. Karin. 1988. Cytosine methylation does not affect binding of transcription factor Spl. Proc. Natl. Acad. Sci. USA 85:2066–2070.
  • Hergersberg, M.%% 1991. Biological aspects of cytosine methylation in eukaryotic cells. Experientia 47:1171–1185.
  • Iguchi-Ariga, S. M. M., and W. Schaffner. 1989. CpG methylation of the cAMP-responsive enhancer/promoter sequence TGACGTCA abolishes specific factor binding as well as transcriptional activation. Genes Dev. 3:612–619.
  • Jost, J.-P., and J. Hofsteenge. 1992. The repressor MDBP-2 is a member of the histone HI family that binds preferentially in vitro and in vivo to methylated nonspecific DNA sequences. Proc. Natl. Acad. Sci. USA 89:9499–9503.
  • Jost, J.-P., H.-P. Saluz, and A. Pawlak. 1991. Estradiol down regulates the binding activity of an avian vitellogenin gene repressor (MDBP-2) and triggers a gradual demethylation of the mCpG pair of its DNA binding site. Nucleic Acids Res. 19:5771–5775.
  • Käs, E., L. Pojjak, Y. Adachi, and U. K. Laemmli. 1993. A model for chromatin opening: stimulation of topoisomerase II and restriction enzyme cleavage of chromatin by distamycin. EMBO J. 12:115–126.
  • Keshet, I., J. Lieman-Hurwitz, and H. Cedar. 1986. DNA methylation affects the formation of active chromatin. Cell 44:535–543.
  • Keshet, I., J. Yisraeli, and H. Cedar. 1985. Effect of regional DNA methylation on gene expression. Proc. Natl. Acad. Sci. USA 82:2560–2564.
  • Levine, A., G. L. Cantoni, and A. Razin. 1991. Inhibition of promoter activity by methylation: possible involvement of protein mediators. Proc. Natl. Acad. Sci. USA 88:6515–6518.
  • Levine, A., G. L. Cantoni, and A. Razin. 1992. Methylation in the preinitiation domain suppresses gene transcription by an indirect mechanism. Proc. Natl. Acad. Sci. USA 89:10119–10123.
  • Lewis, J. D., R. R. Meehan, W. J. Henzel, I. Maurer-Fogy, P. Jeppesen, F. Klein, and A. Bird. 1992. Purification, sequence, and cellular localization of a novel chromosomal protein that binds to methylated DNA. Cell 69:905–914.
  • Lock, L. F., N. Takagi, and G. Martin. 1987. Methylation of the Hprt gene on the inactive X occurs after chromosome inactivation. Cell 48:39–46.
  • Meehan, R. R., J. D. Lewis, and A. P. Bird. 1992. Characterization of MeCP2, a vertebrate DNA binding protein with affinity for methylated DNA. Nucleic Acids Res. 20:5085–5092.
  • Meehan, R. R., J. D. Lewis, S. McKay, E. L. Kleiner, and A. P. Bird. 1989. Identification of a mammalian protein that binds specifically to DNA containing methylated CpGs. Cell 58:499–507.
  • Orend, G., I. Kuhlmann, and W. Doerfler. 1991. Spreading of DNA methylation across integrated foreign (adenovirus type 12) genomes in mammalian cells. J. Virol. 65:4301–4308.
  • Pawlak, A., M. Bryans, and J.-P. Jost. 1991. An avian 40 KDa nucleoprotein binds preferentially to a promoter sequence containing one single pair of methylated CpG. Nucleic Acids Res. 19:1029–1034.
  • Pfeifer, G. P., S. D. Steigerwald, R. S. Hansen, S. M. Gartler, and A. D. Riggs. 1990. Polymerase chain reaction-aided genomic sequencing of an X chromosome-linked CpG island: methylation patterns suggest clonal inheritance, CpG site autonomy, and an explanation of activity state stability. Proc. Natl. Acad. Sci. USA 87:8252–8256.
  • Razin, A., and H. Cedar. 1991. DNA methylation and gene expression. Microbiol. Rev. 55:451–458.
  • Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Sasaki, T., R. S. Hansen, and S. M. Gartler. 1992. Hemimeth-ylation and hypersensitivity are early events in transcriptional reactivation of human inactive X-linked genes in a hamster × human somatic cell hybrid. Mol. Cell. Biol. 12:3819–3826.
  • Seed, B., and J.-Y. Sheen. 1988. A simple phase-extraction assay for chloramphenicol acyltransferase activity. Gene 67: 271–277.
  • Toth, M., U. Lichtenberg, and W. Doerfler. 1989. Genomic sequencing reveals a 5-methylcytosine-free domain in active promoters and the spreading of preimposed methylation patterns. Proc. Natl. Acad. Sci. USA 86:3728–3732.
  • Wolf, S. F., S. Dintzis, D. Toniolo, G. Persico, K. D. Lunnen, J. Axelman, and B. R. Migeon. 1984. Complete concordance between glucose-6-phosphate dehydrogenase activity and hy-pomethylation of 3′ CpG clusters: implications for X chromosome dosage compensation. Nucleic Acids Res. 12:9333–9348.
  • Wolf, S. F., and B. R. Migeon. 1985. Clusters of CpG dinucleotides implicated by nuclease hypersensitivity as control elements of housekeeping genes. Nature (London) 314:467–469.
  • Yisraeli, J., D. Frank, A. Razin, and H. Cedar. 1988. Effect of in vitro DNA methylation on β-globin gene expression. Proc. Natl. Acad. Sci. USA 85:4638–4642.
  • Zhang, X.-Y., C. K. Asiedu, P. C. Supakar, R. Khan, K. C. Ehrlich, and M. Ehrlich. 1990. Binding sites in mammalian genes and viral gene regulatory regions recognized by methylated DNA-binding protein. Nucleic Acids Res. 18:6253–6260.

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