0
Views
6
CrossRef citations to date
0
Altmetric
Transcriptional Regulation

The Saccharomyces cerevisiae Cdc68 Transcription Activator Is Antagonized by San1, a Protein Implicated in Transcriptional Silencing

, &
Pages 7553-7565 | Received 12 Jul 1993, Accepted 09 Sep 1993, Published online: 31 Mar 2023

References

  • Abrams, E., L. Neigeborn, and M. Carlson. 1986. Molecular analysis of SNF2 and SNF5, genes required for expression of glucose-repressible genes in Saccharomyces cerevisiae. Mol. Cell. Biol. 6:3643–3651.
  • Aparicio, O. M., B. L. Billington, and D. E. Gottschling. 1991. Modifiers of position effect are shared between telomeric and silent mating-type loci in S. cerevisiae. Cell 66:1279–1287.
  • Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.). 1991. Current protocols in molecular biology. Wiley Interscience, New York.
  • Berger, S. L., B. Pina, N. Silverman, G. A. Marcus, J. Agapite, J. L. Regier, S. J. Triezenberg, and L. Guarente. 1992. Genetic isolation of ADA2: a potential transcription adaptor required for function of certain acidic activation domains. Cell 70:251–265.
  • Birnboim, H. C., and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513–1523.
  • Boeke, J. D., F. Lacroute, and G. R. Fink. 1984. A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol. Gen. Genet. 197:345–346.
  • Botstein, D., S. C. Falco, S. E. Stewart, M. Brennan, S. Scherer, D. T. Stinchcomb, K. Struhl, and R. W. Davis. 1979. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8:17–24.
  • Clark-Adams, C. D., D. Norris, M. A. Osley, J. S. Fassler, and F. Winston. 1988. Changes in histone gene dosage alter transcription in yeast. Genes Dev. 2:150–159.
  • Clark-Adams, C. D., and F. Winston. 1987. The SPT6 gene is essential for growth and is required for 5-mediated transcription in Saccharomyces cerevisiae. Mol. Cell. Biol. 7:679–686.
  • Durrin, L. K., R. K. Mann, P. S. Kayne, and M. Grunstein. 1991. Yeast histone H4 N-terminal sequence is required for promoter activation in vivo. Cell 65:1023–1031.
  • Elgin, S. C. R.. 1988. The formation and function of DNase I hypersensitive sites in the process of gene activation. J. Biol. Chem. 263:19259–19262.
  • Feinberg, A. P., and B. Vogelstein. 1983. A technique for radiolabeling DNA restriction fragments to high specific activity. Anal. Biochem. 132:6–13.
  • Gill, G., and R. Tjian. 1992. Eukaryotic coactivators associated with the TATA box binding protein. Curr. Opin. Genet. Dev. 2:236–242.
  • Grunstein, M.%% 1990. Histone function in transcription. Annu. Rev. Cell Biol. 6:643–678.
  • Guthrie, C., and G. R. Fink (ed.). 1991. Methods in enzymology, vol. 194. Guide to yeast genetics and molecular biology. Academic Press, Inc., San Diego, Calif.
  • Hadwiger, J. A., C. Wittenberg, H. E. Richardson, M. de Barros Lopes, and S. I. Reed. 1989. A family of cyclin homologs that control the G1 phase in yeast. Proc. Natl. Acad. Sci. USA 86:6255–6259.
  • Hartwell, L. H.. 1967. Macromolecule synthesis in temperature-sensitive mutants of yeast. J. Bacteriol. 93:1662–1670.
  • Hashimoto, H., Y. Kikuchi, Y. Nogi, and T. Fukasawa. 1983. Regulation of expression of the galactose gene cluster in Saccharomyces cerevisiae: isolation and characterization of the regulatory gene GAL4. Mol. Gen. Genet. 191:31–38.
  • Hill, J. E., A. M. Myers, T. J. Koemer, and A. Tzagoloff. 1986. Yeast/E. coli shuttle vectors with multiple restriction sites. Yeast 2:163–167.
  • Hinnen, A., J. B. Hicks, and G. R. Fink. 1978. Transformation of yeast. Proc. Natl. Acad. Sci. USA 75:1929–1933.
  • Holmes, D. S., and M. Quigley. 1981. A rapid boiling method for the preparation of bacterial plasmids. Anal. Biochem. 114:193–197.
  • Ito, H., Y. Fukuda, K. Murata, and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163–168.
  • Jiang, Y. W., and D. J. Stillman. 1992. Involvement of the SIN4 global transcriptional regulator in the chromatin structure of Saccharomyces cerevisiae. Mol. Cell. Biol. 12:4503–4514.
  • Johnson, L. M., P. S. Kayne, E. S. Kahn, and M. Grunstein. 1990. Genetic evidence for an interaction between SIR3 and Histone H4 in the repression of the silent mating loci in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 87: 6286–6290.
  • Johnson, P. F., and S. L. McKnight. 1989. Eukaryotic transcriptional regulatory proteins. Annu. Rev. Biochem. 58:799–839.
  • Johnston, G. C., J. R. Pringle, and L. H. Hartwell. 1977. Coordination of growth and cell division in the budding yeast Saccharomyces cerevisiae. Exp. Cell Res. 105:79–98.
  • Johnston, S. A., and J. E. Hopper. 1982. Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon. Proc. Natl. Acad. Sci. USA 79: 6971–6975.
  • Kruger, W., and I. Herskowitz. 1991. A negative regulator of HO transcription, SIN1 (SPT2), is a nonspecific DNA-binding protein related to HMG1. Mol. Cell. Biol. 11:4135–4146.
  • Laurenson, P., and J. Rine. 1992. Silencers, silencing, and heritable transcriptional states. Microbiol. Rev. 56:543–560.
  • Lewin, B.%% 1990. Commitment and activation at Pol II promoters: a tail of protein-protein interactions. Cell 61:1161–1164.
  • Lorincz, A.%% 1984. Quick preparation of plasmid DNA from yeast. Focus (Bethesda Research Laboratories) 6:11.
  • Malone, E. A., C. D. Clark, A. Chiang, and F. Winston. 1991. Mutations in SPT16/CDC68 suppress cis- and trans-acting mutations that affect promoter function in Saccharomyces cerevisiae. Mol. Cell. Biol. 11:5710–5717.
  • Malone, E. A., J. S. Fassler, and F. Winston. 1993. Molecular and genetic characterization of SPT4, a gene important for transcription initiation in Saccharomyces cerevisiae. Mol. Gen. Genet. 237:449–459.
  • Messing, J.%% 1983. New M13 vectors for cloning. Methods Enzymol. 101:20–78.
  • Mitchell, P. J., and R. Tjian. 1989. Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. Science 245:371–378.
  • Mortimer, R. K., C. R. Contopoulou, and J. S. King. 1992. Genetic and physical maps of Saccharomyces cerevisiae, edition 11. Yeast 8:829–832.
  • Mortimer, R. K., and D. Schild. 1985. Genetic map of Saccharomyces cerevisiae, edition 9. Microbiol. Rev. 49:181–212.
  • Nasmyth, K. A.. 1982. The regulation of yeast mating-type chromatin structure by SIR: an action at a distance affecting both transcription and transposition. Cell 30:567–578.
  • Nasmyth, K. A.. 1990. FAR-reaching discoveries about the regulation of START. Cell 63:1117–1120.
  • Neigeborn, L., J. L. Celenza, and M. Carlson. 1987. SSN20 is an essential gene with mutant alleles that suppress defects in SUC2 transcription in Saccharomyces cerevisiae. Mol. Cell. Biol. 7:672–678.
  • Nogi, Y., H. Shimada, Y. Matsuzaki, H. Hashimoto, and T. Fukasawa. 1984. Regulation of expression of the galactose gene cluster in Saccharomyces cerevisiae. II. The isolation and dosage effect of the regulatory gene GAL80. Mol. Gen. Genet. 195:29–34.
  • Norrander, J., T. Kempe, and J. Messing. 1983. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene 26:101–106.
  • Osley, M. A.. 1991. The regulation of histone synthesis in the cell cycle. Annu. Rev. Biochem. 60:827–861.
  • Osley, M. A., and D. Lycan. 1987. trans-acting regulatory mutations that alter transcription of Saccharomyces cerevisiae histone genes. Mol. Cell. Biol. 7:4204–4210.
  • Park, E.-C., and J. W. Szostak. 1990. Point mutations in the yeast histone H4 gene prevent silencing of the silent mating type locus HML. Mol. Cell. Biol. 10:4932–4934.
  • Penn, M. D., G. Thireos, and H. Greer. 1984. Temporal analysis of general control of amino acid biosynthesis in Saccharomyces cerevisiae: role of positive regulatory genes in initiation and maintenance of mRNA derepression. Mol. Cell. Biol. 4:520–528.
  • Pearson, W. R.. 1990. Rapid and sensitive sequence comparison with FASTP and FASTA. Methods Enzymol. 188:63–98.
  • Prendergast, J. A., L. E. Murray, A. Rowley, D. R. Carruthers, R. A. Singer, and G. C. Johnston. 1990. Size selection identifies new genes that regulate Saccharomyces cerevisiae cell proliferation. Genetics 124:81–89.
  • Richardson, H. E., C. Wittenberg, F. Cross, and S. I. Reed. 1989. An essential G1 function for cyclin-like proteins in yeast. Cell 59:1127–1133.
  • Rine, J., and I. Herskowitz. 1987. Four genes responsible for a position effect on expression from HML and HMR in Saccharomyces cerevisiae. Genetics 116:9–22.
  • Rothstein, R.%% 1983. One step gene disruption in yeast. Methods Enzymol. 101:202–211.
  • Rowley, A., R. A. Singer, and G. C. Johnston. 1991. CDC68, a yeast gene that affects regulation of cell proliferation and transcription, encodes a protein with a highly acidic carboxyl terminus. Mol. Cell. Biol. 11:5718–5726.
  • Rowley, A., R. A. Singer, and G. C. Johnston. Unpublished data.
  • Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463–5467.
  • Sawadogo, M., and A. Sentenac. 1990. RNA polymerase B (II) and general transcription factors. Annu. Rev. Biochem. 59:711–754.
  • Schnell, R. L. D’Ari, M. Foss, D. Goodman, and J. Rine. 1989. Genetic and molecular characterization of suppressors of SIR4 mutations in Saccharomyces cerevisiae. Genetics 122:29–46.
  • Sikorski, R. S., and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19–27.
  • Silverman, S. J., and G. R. Fink. 1984. Effects of Ty insertions on HIS4 transcription in Saccharomyces cerevisiae. Mol. Cell. Biol. 4:1246–1251.
  • Simpson, R. T.. 1991. Nucleosome positioning: occurrence, mechanisms and functional consequences. Prog. Nucleic Acid Res. Mol. Biol. 40:143–184.
  • Swanson, M. S., E. A. Malone, and F. Winston. 1991. SPT5, an essential gene important for normal transcription in Saccharomyces cerevisiae, encodes an acidic nuclear protein with a carboxy-terminal repeat. Mol. Cell. Biol. 11:3009–3019.
  • Swanson, M. S., and F. Winston. 1992. SPT4, SPT5 and SPT6 interactions: effects on transcription and viability in Saccharomyces cerevisiae. Genetics 132:325–336.
  • Thomas, P. S.. 1983. Hybridization of denatured RNA transferred or dotted to nitrocellulose paper. Methods Enzymol. 100:255–266.
  • Varshavsky, A.%% 1992. The N-end rule. Cell 69:725–735.
  • Winston, F., and M. Carlson. 1992. Yeast SNF/SWI transcription activators and the SPT/SIN chromatin connection. Trends Genet. 8:387–391.
  • Winston, F., D. T. Chaleff, B. Valent, and G. R. Fink. 1984. Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107:179–197.
  • Wittenberg, C., K. Sugimoto, and S. I. Reed. 1990. Gl-specific cyclins of S. cerevisiae: cell cycle periodicity, regulation by mating pheromone, and association with the p34CDC28 protein kinase. Cell 62:225–237.
  • Xu, H., U. Kim, T. Schuster, and M. Grunstein. 1992. Identification of a new set of cell cycle-regulatory genes that regulate S-phase transcription of histone genes in Saccharomyces cerevisiae. Mol. Cell. Biol. 12:5249–5259.
  • Xu, Q., G. C. Johnston, and R. A. Singer. Unpublished data.
  • Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp8 and pUC19 vectors. Gene 33:103–119.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.