Disruption of Largest Subunit RNA Polymerase II Genes in Trypanosoma Brucei

REFERENCES

• Agabian, N. 1990. Trans splicing of nuclear pre-mRNAs. Cell 61:1157–1160.
   PubMedGoogle Scholar
• Allison, L. A., M. Moyle, M. Shales, and C. J. Ingles. 1985. Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases. Cell 42:599–610.
   PubMed Web of Science ®Google Scholar
• Archambault, J., M. A. Drebot, J. C. Stone, and J. D. Friesen. 1992. Isolation and phenotypic analysis of conditional-lethal, linker-insertion mutations in the gene encoding the largest subunit of RNA polymerase II in Saccharomyces cerevisiae. Mol. Gen. Genet. 232:408–414.
   PubMedGoogle Scholar
• Banerjee, A. K. 1980. 5′-terminal cap structure in eukaryotic messenger ribonucleic acids. Microbiol. Rev. 44:175–205.
   PubMedGoogle Scholar
• Bartolomei, M. S., and J. L. Corden. 1987. Localization of α-amanitin resistance mutation in the gene encoding the largest subunit of mouse RNA polymerase II. Mol. Cell. Biol. 7:586–594.
   PubMedGoogle Scholar
• Bernards, A., L. H. T. Van der Ploeg, A. C. C. Frasch, and P. Borst. 1981. Activation of trypanosome surface glycoprotein genes involves a duplication-transposition leading to an unaltered 3′ end. Cell 27:497–505.
   PubMedGoogle Scholar
• Bird, D. M., and D. L. Riddle. 1989. Molecular cloning and sequencing of ama-1, the gene encoding the largest subunit of Caenorhabditis elegans RNA polymerase II. Mol. Cell. Biol. 9:4119–4130.
   PubMedGoogle Scholar
• Borst, P. 1986. Discontinuous transcription and antigenic variation in trypanosomes. Annu. Rev. Biochem. 55:701–732.
   PubMed Web of Science ®Google Scholar
• Borst, P. 1991. Molecular genetics of antigenic variation. Immunol. Today 12:A29–A33.
   Google Scholar
• Brown, S. D., J. Huang, and L. H. T. Van der Ploeg. 1992. The promoter for the procyclic acidic repetitive protein (PARP) genes of Trypanosoma brucei shares features with RNA polymerase I promoters. Mol. Cell. Biol. 12:2644–2652.
   PubMedGoogle Scholar
• Brun, R., and M. Schonenberger. 1979. Cultivation and in vitro cloning of procyclic culture from Trypanosoma brucei in a semidefined medium. Acta Trop. 36:289–292.
   PubMed Web of Science ®Google Scholar
• Capbern, A., C. Giroud, T. Baltz, and P. Mattern. 1977. Trypanosoma equiperdum: étude des variations antigéniques au cours de la trypanosomose expérimentale du lapin. Exp. Parasitol. 42:6–13.
   PubMedGoogle Scholar
• Chung, H.-M., M. G.-S. Lee, and L. H. T. Van der Ploeg. 1992. RNA polymerase I-mediated protein coding gene expression in Trypanosoma brucei. Parasitol. Today 8:414–418.
   Google Scholar
• Clayton, C., et al. Unpublished data.
   Google Scholar
• Cornelissen, A. W. C. A., R. Evers, E. Grondal, A. Hammer, W. Jess, and J. Köck. 1989. Transcription and RNA polymerases in Trypanosoma brucei. Acta Leiden. 58:2–23.
   Google Scholar
• Evers, R., A. Hammer, J. Kock, W. Jess, P. Borst, S. Memet, and A. W. C. A. Cornelissen. 1989. Trypanosoma brucei contains two RNA polymerase II largest subunit genes with an altered C-terminal domain. Cell 56:585–597.
   PubMedGoogle Scholar
• Fletcher, L., S. D. Corbin, K. S. Browning, and J. M. Ravel. 1990. The absence of a m7G cap on β-globin mRNA and alfalfa mosaic virus RNA 4 increases the amounts of initiation factor 4F required for translation. J. Biol. Chem. 265:19582–19587.
   PubMedGoogle Scholar
• Gottesdiener, K., H.-M. Chung, S. D. Brown, M. G.-S. Lee, and L. H. T. Van der Ploeg. 1991. Characterization of VSG gene expression site promoters and promoter-associated DNA rearrangement events. Mol. Cell. Biol. 11:2467–2477.
   PubMedGoogle Scholar
• Gottesdiener, K., J. Garciá-Ánoveros, M. G.-S. Lee, and L. H. T. Van der Ploeg. 1990. Chromosome organization of the protozoan Trypanosoma brucei. Mol. Cell. Biol. 10:6079–6083.
   PubMedGoogle Scholar
• Greenleaf, A. L., J. R. Weeks, R. A. Voelker, S. Ohnishi, and B. Dickson. 1980. Genetic and biochemical characterization of mutants at an RNA polymerase II locus in D. melanogaster. Cell 21:785–792.
   PubMedGoogle Scholar
• Grondal, E. J., R. Evers, K. Kosubek, and A. W. C. A. Cornelissen. 1989. Characterization of the RNA polymerases of Trypanosoma brucei: trypanosomal mRNAs are composed of transcripts derived from both RNA polymerase II and III. EMBO J. 8:3383–3389. (Erratum, 8:4359.)
   PubMedGoogle Scholar
• Grummt, I., and J. A. Skinner. 1985. Efficient transcription of a protein-coding gene from the RNA polymerase I promoter in transfected cells. Proc. Natl. Acad. Sci. USA 82:722–726.
   PubMedGoogle Scholar
• Hamm, J., E. Darzynkiewicz, S. M. Tahara, and I. W. Mattaj. 1990. The trimethylguanosine cap structure of U1 snRNA is a component of a bipartite nuclear targeting signal. Cell 62:569–577.
   PubMed Web of Science ®Google Scholar
• Hamm, J., and I. W. Mattaj. 1990. Monomethylated cap structures facilitate RNA export from the nucleus. Cell 63:109–118.
   PubMed Web of Science ®Google Scholar
• Ingles, C. J., H. J. Himmelfarb, M. Shales, and A. L. Greenleaf. 1984. Identification, molecular cloning, and mutagenesis of Saccharomyces cerevisiae RNA polymerase genes. Proc. Natl. Acad. Sci. USA 81:2157–2161.
   PubMed Web of Science ®Google Scholar
• Jokerst, R. S., J. R. Weeks, W. A. Zehring, and A. L. Greenleaf. 1989. Analysis of the gene encoding the largest subunit of RNA polymerase II in Drosophila. Mol. Gen. Genet. 215:266–275.
   PubMedGoogle Scholar
• Kolodziej, P. A., and R. A. Young. 1991. Mutations in the three largest subunits of yeast RNA polymerase II that affect enzyme assembly. Mol. Cell. Biol. 11:4669–4678.
   PubMedGoogle Scholar
• Kooter, J. M., and P. Borst. 1984. α-Amanitin-insensitive transcription of variant surface glycoprotein genes provides further evidence for discontinuous transcription in trypanosomes. Nucleic Acids Res. 12:9457–9472.
   PubMedGoogle Scholar
• Lee, M. G.-S., and L. H. T. Van der Ploeg. 1990. Homologous recombination and stable transfection in the parasitic protozoan Trypanosoma brucei. Science 250:1583–1587.
   PubMed Web of Science ®Google Scholar
• Lee, M. G.-S., and L. H. T. Van der Ploeg. 1991. The hygromy- cin B-resistance-encoding gene as a selectable marker for stable transformation of Trypanosoma brucei. Gene 105:255–257.
   PubMedGoogle Scholar
• Lopata, M. A., D. W. Cleveland, and B. Sollner-Webb. 1986. RNA polymerase specificity of mRNA production and enhancer action. Proc. Natl. Acad. Sci. USA 83:6677–6681.
   PubMedGoogle Scholar
• Mowatt, M. R., and C. E. Clayton. 1987. Developmental regulation of a novel repetitive protein of Trypanosoma brucei. Mol. Cell. Biol. 7:2838–2844.
   PubMedGoogle Scholar
• Mowatt, M. R., and C. E. Clayton. 1988. Polymorphism in the procyclic acidic repetitive protein gene family of Trypanosoma brucei. Mol. Cell. Biol. 8:4055–4062.
   PubMedGoogle Scholar
• Murphy, W., J. Knol, P. Watkins, and N. Agabian. 1986. Identification of a novel Y branch structure as an intermediate in trypanosome mRNA processing: evidence for trans-splicing. Cell 47:517–525.
   PubMedGoogle Scholar
• Pays, E., H. Coquelet, P. Tebabi, A. Pays, D. Jefferies, M. Steinert, E. Koenig, R. O. Williams, and I. Roditi. 1990. Trypanosoma brucei: constitutive activity of the VSG and procyclin gene promoters. EMBO J. 9:3145–3151.
   PubMedGoogle Scholar
• Roditi, I., M. Carrington, and M. Turner. 1987. Expression of a polypeptide containing a dipeptide repeat is confined to the insect stage of Trypanosoma brucei. Nature (London) 325:272–274.
   PubMedGoogle Scholar
• Rogalski, T. M., A. M. E Bulleijahn, and D. L. Riddle. 1988. Lethal and amanitin-resistance mutations in the Caenorhabditis elegans ama-1 and ama-2 genes. Genetics 120:409–422.
   PubMedGoogle Scholar
• Rogalski, T. M., M. Golomb, and D. L. Riddle. 1990. Mutant Caenorhabditis elegans RNA polymerase II with a 20,000-fold reduced sensitivity to α-amanitin. Genetics 126:889–898.
   PubMedGoogle Scholar
• Rudenko, G., D. Bishop, K. Gottesdiener, and L. H. T. Van der Ploeg. 1989. Alpha-amanitin resistant transcription of protein coding genes in insect and bloodstream form Trypanosoma brucei. EMBO J. 8:4259–4263.
   PubMedGoogle Scholar
• Rudenko, G., H.-M. Chung, V. P. Pham, and L. H. T. Van der Ploeg. 1991. RNA polymerase I can mediate expression of CAT and neo protein-coding genes in Trypanosoma brucei. EMBO J. 10:3387–3397.
   PubMedGoogle Scholar
• Rudenko, G., S. Le Blancq, J. Smith, M. G. S. Lee, A. Rattray, and L. H. T. Van Der Ploeg. 1990. Procyclic acidic repetitive protein (PARP) genes located in an unusually small α-amanitin- resistant transcription unit: PARP promoter activity assayed by transient DNA transfection of Trypanosoma brucei. Mol. Cell. Biol. 10:3492–3504.
   PubMedGoogle Scholar
• Rudenko, G., M. G.-S. Lee, and L. H. T. Van der Ploeg. 1992. The PARP and VSG genes of Trypanosoma brucei do not resemble RNA polymerase II transcription units, as deduced from their resistance to addition of Sarkosyl in nuclear run-on assays. Nucleic Acids Res. 20:303–306.
   PubMedGoogle Scholar
• Rudenko, G., and L. H. T. Van der Ploeg. 1989. Transcription of telomere repeats in protozoa. EMBO J. 8:2633–2638.
   PubMedGoogle Scholar
• Salditt-Georgieff, M., M. Harpold, S. Chen-Kiang, and J. E. Darnell, Jr. 1980. The addition of 5′ cap structures occurs early in hnRNA synthesis and prematurely terminated molecules are capped. Cell 19:69–78.
   PubMed Web of Science ®Google Scholar
• Sambrook, J., E. F. Fritsch, and T. Maniatis. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
   Google Scholar
• Scafe, C., M. Nonet, and R. A. Young. 1990. RNA polymerase II mutants defective in transcription of a subset of genes. Mol. Cell. Biol. 10:1010–1016.
   PubMed Web of Science ®Google Scholar
• Scheer, U., and R. Benavente. 1990. Functional and dynamic aspects of the mammalian nucleolus. BioEssays 12:14–21.
   PubMedGoogle Scholar
• Shea, C., M. G.-S. Lee, and L. H. T. Van der Ploeg. 1987. VSG gene 118 is transcribed from a cotransposed pol I-like promoter. Cell 50:603–612.
   PubMedGoogle Scholar
• Sherman, D. R., L. Janz, M. Hug, and C. Clayton. 1991. Anatomy of the PARP gene promoter of Trypanosoma brucei. EMBO J. 10:3379–3386.
   PubMedGoogle Scholar
• Smale, S. T., and R. Tjian. 1985. Transcription of herpes simplex virus tk sequences under the control of wild-type and mutant human RNA polymerase I promoters. Mol. Cell. Biol. 5:352–362.
   PubMed Web of Science ®Google Scholar
• Smith, J. L., J. R. Levin, C. J. Ingles, and N. Agabian. 1989. In trypanosomes the homolog of the largest subunit of RNA polymerase II is encoded by two genes and has a highly unusual C-terminal domain structure. Cell 56:815–827.
   PubMedGoogle Scholar
• Sollner-Webb, B., and E. B. Mougey. 1991. News from the nucleolus: rRNA gene expression. Trends Biochem. Sci. 16:58–62.
   PubMedGoogle Scholar
• Steeg, C. M., J. Ellis, and A. Bernstein. 1990. Introduction of specific mutations into RNA polymerase II by gene targeting in mouse embryonic stem cells: evidence for a DNA mismatch repair mechanism. Proc. Natl. Acad. Sci. USA 87:4680–4684.
   PubMedGoogle Scholar
• Surmacz, E., O. Ronning, L. Kaczmarek, and R. Baserga. 1986. Regulation of the expression of the SV40 T-antigen coding gene under the control of an rDNA promoter. J. Cell. Physiol. 127:357–365.
   PubMedGoogle Scholar
• Sutton, R. E., and J. C. Boothroyd. 1986. Evidence for transsplicing in trypanosomes. Cell 47:527–535.
   PubMedGoogle Scholar
• Van der Ploeg, L. H. T. 1990. Antigenic variation in African trypanosomes: genetic recombination and transcriptional control of VSG genes, p. 51–97. IRL Press, Oxford.
   Google Scholar
• Van der Ploeg, L. H. T. 1991. Control of antigenic variation in African trypanosomes. New Biol. 3:324–330.
   PubMedGoogle Scholar
• Verlaan-de Vries, M., M. E. Bogaard, H. van den Elst, J. H. van Boom, S. van der Eb, and J. L. Bos. 1986. A dot-blot screening procedure for mutated ras oncogenes using synthetic oligodeoxynucleotides. Gene 50:313–320.
   PubMed Web of Science ®Google Scholar
• Vickerman, K. 1978. Antigenic variation in trypanosomes. Nature (London) 273:613–617.
   PubMedGoogle Scholar
• Young, R. A. 1991. RNA polymerase II. Annu. Rev. Biochem. 60:689–715.
   PubMed Web of Science ®Google Scholar
• Zomerdijk, J. C. B. M., R. Kieft, and P. Borst. 1991. Efficient production of functional mRNA mediated by RNA polymerase I in Trypanosoma brucei. Nature (London) 353:772–775.
   PubMedGoogle Scholar