Purification of the Human NF-E2 Complex: cDNA Cloning of the Hematopoietic Cell-Specific Subunit and Evidence for an Associated Partner

REFERENCES

• Abate, C., L. Patel, F. J. Rauscher III, and T. Curran. 1990. Redox regulation of Fos and Jun DNA-binding activity in vitro. Science 249:1157–1161.
   PubMed Web of Science ®Google Scholar
• Andrews, N. C., H. Erdjument-Bromage, Μ. B. Davidson, P. Tempst, and S. H. Orkin. 1993. Globin locus control region enhancer-binding factor (NF-E2): an hematopoietic-specific ba- sic-leucine zipper protein. Nature (London) 362:722–728.
   PubMed Web of Science ®Google Scholar
• Behringer, R. R., T. Μ. Ryan, R. D. Palmiter, R. L. Brinster, and T. Μ. Townes. 1990. Human gamma- to beta-globin gene switching in transgenic mice. Genes Dev. 4:380–389.
   PubMed Web of Science ®Google Scholar
• Caterina, J. J., T. M. Ryan, K. Μ. Pawlick, R. D. Palmiter, R. L. Brinster, R. R. Behringer, and T. M∙ Townes. 1991. Human beta-globin locus control region: analysis of the 5’ DNase I hypersensitive site HS 2 in transgenic mice. Proc. Natl. Acad. Sci. USA 88:1626–1630.
   PubMed Web of Science ®Google Scholar
• Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloro- foπn extraction. Anal. Biochem. 162:156–159.
   PubMed Web of Science ®Google Scholar
• Cox, T. C., Μ. J. Bawden, A. Martin, and B. K. May. 1991. Human erythroid 5-aminolevulinate synthase: promoter analysis and identification of an iron-responsive element in the mRNA. EMBO J. 10:1891–1902.
   PubMed Web of Science ®Google Scholar
• Dignam, J. D., R. Μ. Lebovitz, and R. G. Roeder. 1983. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11:1475–1489.
   PubMed Web of Science ®Google Scholar
• Ellis, J., D. Talbot, N. DiUon, and F. Grosveld. 1993. Synthetic human beta-globin 5’HS2 constructs function as locus control regions only in multicopy transgene concatamers. EMBO J. 12:127–134.
   PubMedGoogle Scholar
• Enver, T., N. Raich, A. J. Ebens, T. Papayannopoulou, F. Constantini, and G. Stamatoyannopoulos. 1990. Developmental regulation of human fetal-to-adult globin gene switching in transgenic mice. Nature (London) 344:309–313.
   PubMed Web of Science ®Google Scholar
• Forrester, W. C., E. Epner, Μ. C. Driscoll, T. Enver, Μ. Brice, T. Papayannopoulou, and Μ. Groudine. 1990. A deletion of the human beta-globin locus activation region causes a major alteration in chromatin structure and replication across the entire beta-globin locus. Genes Dev. 4:1637–1649.
   PubMed Web of Science ®Google Scholar
• Forrester, W. C., C. Thompson, J. T. Elder, and Μ. Groudine. 1986. A developmentally stable chromatin structure in the human beta-globin gene cluster. Proc. Natl. Acad. Sci. USA 83:1359–1363.
   PubMed Web of Science ®Google Scholar
• Fraser, P., S. Pruzina, Μ. Antoniou, and F. Grosveld. 1993. Each hypersensitive site of the human beta-globin locus control region confers a different developmental pattern of expression on the globin genes. Genes Dev. 7:106–113.
   PubMed Web of Science ®Google Scholar
• Grosveld, F., G. Blom van Assendelft, D. R. Greaves, and G. Kollias. 1987. Position-independent, high-level expression of the human beta-globin gene in transgenic mice. Cell 51:975–985.
   PubMed Web of Science ®Google Scholar
• Hager, D. A., and R. R. Burgess. 1980. Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA polymerase, and other enzymes. Anal. Biochem. 109:76–86.
   PubMed Web of Science ®Google Scholar
• Hanscombe, O., D. Whyatt, P. Fraser, N. Yannoutsos, D. Greaves, N. Dillon, and F. Grosveld. 1991. Importance of globin gene order for correct developmental expression. Genes Dev. 5:1387–1394.
   PubMedGoogle Scholar
• Harrison, D. E. 1972. Marrow transplantation and iron therapy in mouse hereditary microcytic anemia. Blood 40:893–901.
   PubMedGoogle Scholar
• Jarman, A. P., W. G. Wood, J. A. Sharpe, G. Gordon, H. Ayyub, and D. R. Higgs. 1991. Characterization of the major regulatory element upstream of the human alpha-globin gene cluster. Mol. Cell. Biol. 11:4679–4689.
   PubMed Web of Science ®Google Scholar
• Kadanoga, J. T., and R. Tjian. 1986. Affinity purification of sequence-specific DNA binding proteins. Proc. Natl. Acad. Sci. USA 83:5889–5893.
   PubMedGoogle Scholar
• Kerppola, T. K∙, and T. Curran. 1991. Transcription factor interactions: basics on zippers. Curr. Opin. Struct. Biol. 1:7179.
   Google Scholar
• Li, Q., B. Zhou, P. Powers, T. Enver, and G. Stamatoyannopoulos. 1990. Beta-globin locus activation regions: ∞nservation of organization, structure, and function. Proc. Natl. Acad. Sci. USA 87:8207–8211.
   PubMedGoogle Scholar
• Li, R., J. D. Knight, S. P. Jackson, R. Tjian, and M. R. Botchan. 1991. Direct interaction between Spl and the BPV enhancer E2 protein mediates synergistic activation of transcription. Cell 65:493–505.
   PubMedGoogle Scholar
• Lichter, P., C. C. Tang, K. Call, G. Hermanson, G. A. Evans, D. Housman, and D∙ C. Ward. 1990. High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 247:64–69.
   PubMed Web of Science ®Google Scholar
• Liu, D., J. C. Chang, P. Moi, W. Liu, Y. W. Kan, and P. T. Curtin. 1992. Dissection of the enhancer activity of beta-globin 5’ DNase I-hypersensitive site 2 in transgenic mice. Proc. Natl. Acad. Sci. USA 89:3899–3903.
   PubMed Web of Science ®Google Scholar
• Lowrey, C. L. Personal communication.
   Google Scholar
• Lozzio, C. B∙, and B. B. Lozzio. 1975. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood 45:321–334.
   PubMed Web of Science ®Google Scholar
• Martin, P., and T. Papayannopoulou. 1982. HEL cells: a new human erythroleukemia cell line with spontaneous and induced globin gene expression. Science 216:1233–1235.
   PubMed Web of Science ®Google Scholar
• Maxam, A. Μ., and W. Gilbert. 1980. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 65:499–560.
   PubMedGoogle Scholar
• Mermod, N., E. A. O’Neill, T. J. Kelly, and R. Tjian. 1989. The proline-rich transcriptional activator of CTF/NF-1 is distinct from the replication and DNA binding domain. Cell 58:741–753.
   PubMed Web of Science ®Google Scholar
• Mignotte, V., J. F. Eleouet, N. Raich, and P. H. Romeo. 1989. Cis- and trans-acting elements involved in the regulation of the erythroid promoter of the human porphobilinogen deaminase gene. Proc. Natl. Acad. Sci. USA 86:6548–6552.
   PubMed Web of Science ®Google Scholar
• Mignotte, V., L. Wall, E. deBoer, F. Grosveld, and P. H. Romeo. 1989. Two tissue-specific factors bind the erythroid promoter of the human porphobilinogen deaminase gene. Nucleic Acids Res. 17:37–54.
   PubMed Web of Science ®Google Scholar
• Miller, J. L., C. E. Walsh, P. A. Ney, R. J. Samulski, and A. W. Nienhuis. Single copy transduction and expression of human gamma-globin in K562 erythroleukemia cells using recombinant adeno-associated virus vectors: the effect of mutations in NF-E2 and GATA-I binding motifs within the HS2 enhancer. Blood, in press.
   Google Scholar
• Moon, A. Μ., and T. J. Ley. 1990. Conservation of the primary structure, organization, and function of the human and mouse beta-globin locus-activating regions. Proc. Natl. Acad. Sci. USA 87:7693–7697.
   PubMedGoogle Scholar
• Ney, P. A., B. P. Sorrentino, C. H. Lowrey, and A. W. Nienhuis. 1990. Inducibility of the HS II enhancer depends on binding of an erythroid specific nuclear protein. Nucleic Acids Res. 18:6011–6017.
   PubMed Web of Science ®Google Scholar
• Ney, P. A., B. P. Sorrentino, K. T. McDonagh, and A. W. Nienhuis. 1990. Tandem AP-I binding sites within the human beta-globin dominant control region function as an inducible enhancer in erythroid cells. Genes Dev. 4:993–1006.
   PubMed Web of Science ®Google Scholar
• Ochs, D. 1983. Protein contaminants of sodium dodecyl sulfatepolyacrylamide gels. Anal. Biochem. 135:470–474.
   PubMed Web of Science ®Google Scholar
• Pearson, R. B., and B. Kemp. 1990. Protein kinase phosphorylation site sequences and consensus specificity motifs: tabula-tions. Methods Enzymol. 200:62–81.
   Google Scholar
• Peters, L. L., N. C. Andrews, E. M. Eicher, Μ. B. Davidson, S. H. Orkin, and S. Lux. 1993. The hematopoietic transcription factor NF-E2 is the site of the hypochromic, microcytic anemia mutation, mk, located on mouse chromosome 15. Nature (London) 362:768–770.
   PubMedGoogle Scholar
• Philipsen, S., D. Talbot, P. Fraser, and F. Grosveld. 1990. The beta-globin dominant control region: hypersensitive site 2. EMBO J. 9:2159–2167.
   PubMed Web of Science ®Google Scholar
• Pruzina, S., O. Hanscombe, D. Whyatt, F. Grosveld, and S. Philipsen. 1991. Hypersensitive site 4 of the human beta-globin locus control region. Nucleic Adds Res. 19:1413–1419.
   PubMed Web of Science ®Google Scholar
• Reitman, M., and G. Felsenfeld. 1988. Mutational analysis of the chicken beta-globin enhancer reveals two positive-acting domains. Proc. Natl. Acad. Sci. USA 85:6267–6271.
   PubMedGoogle Scholar
• Romeo, P. H., Μ. H. Prandini, V. Joulin, V. Mignotte, M∙ Prenant, W. Vainchenker, G. Marguerie, and G. Uzan. 1990. Megakaryocytic and erythrocytic lineages share specific transcription factors. Nature (London) 344:447–449.
   PubMed Web of Science ®Google Scholar
• Rooney, R. J., P. Raychaudhuri, and J. R. Nevins. 1990. E4F and ATF, two transcription factors that recognize the same site, can be distinguished both physically and functionally: a role for E4F in E1A trans-activation. Mol. Cell. Biol. 10:5138–5149.
   PubMedGoogle Scholar
• Rnssell, E. S. 1979. Hereditary anemias of the mouse: a review for geneticists. Adv. Genet. 20:357–459.
   PubMedGoogle Scholar
• Russell, E. S., D. J. Nash, S. E. Bernstein, E. L. Kent, E. C. McFarIand, S. Μ. Matthews, and Μ. S. Norwood. 1970. Characterization and genetic studies of microcytic anemia in house mouse. Blood 35:838–850.
   PubMedGoogle Scholar
• Sambrook, J., E∙ F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
   Google Scholar
• Solomon, W. B., C. Lin, J. Palma, X. Y. Gao, and S. Wu. 1993. Suppression of a cellular differentiation program by phorbol esters coincides with inhibition of binding of a cell-specific transcription factor (NF-E2) to an enhancer element required for expression of an erythroid-specific gene. J. Biol. Chem. 268:5089–5096.
   PubMedGoogle Scholar
• Sorrentino, B., P. Ney, D. Bodine, and A. W. Nienhuis. 1990. A 46 base pair enhancer sequence within the locus activating region is required for induced expression of the gamma-globin gene during erythroid differentiation. Nucleic Acids Res. 18:2721–2731.
   PubMedGoogle Scholar
• Stevens, P. W., J. B. Dodgson, and J. D. Engel. 1987. Structure and expression of the chicken ferritin H-subunit gene. Mol. Cell. Biol. 7:1751–1758.
   PubMed Web of Science ®Google Scholar
• Sundstrom, C., and K. Nilsson. 1976. Establishment and characterization of a human histiocytic lymphoma cell line (U-937). Int. J. Cancer 17:565–577.
   PubMed Web of Science ®Google Scholar
• Taketani, S., J. Inazawa, Y. Nakahashi, T. Abe, and R. Tokunaga. 1992. Structure of the human ferrochetalase gene: Exon/intron gene organization and location of the gene to chromosome 18. Eur. J. Biochem. 205:217–222.
   PubMedGoogle Scholar
• Talbot, D., S. Philipsen, P. Fraser, and F. Grosveld. 1990. Detailed analysis of the site 3 region of the human beta-globin dominant control region. EMBO J. 9:2169–2178.
   PubMed Web of Science ®Google Scholar