4
Views
16
CrossRef citations to date
0
Altmetric
Research Article

Isolation and Characterization of the SPT2 Gene, a Negative Regulator of Ty-Controlled Yeast Gene Expression

, , &
Pages 1543-1553 | Received 09 Nov 1984, Accepted 26 Mar 1985, Published online: 31 Mar 2023

LITERATURE CITED

  • Adams, S. P., Kavka K. S., Wykes E. J., Holder S. B., and Gallupi G. R.. 1983. Hindered dialkylamino nucleoside phosphite reagents in the synthesis of two DNA 51-mers. J. Am. Chem. Soc. 105:661–663.
  • Anderson, W. F., Ohlendorf D. H., Takeda Y., and Matthews B. W.. 1981. Structure of the cro repressor from bacteriophage λ and its interaction with DNA. Nature London 290:754–758.
  • Astell, C. R., Ahlstrom-Jonasson L., Smith M., Tatchell K., Nasmyth K. A., and Hall B. D.. 1981. The sequence of the DNAs coding for the mating-type loci of Saccharomyces cerevisiae. Cell 27:15–23.
  • Bennetzen, J. L., and Hall B. D.. 1982. Codon selection in yeast. J. Biol. Chem. 257:3026–3031.
  • Botstein, D., and Davis R. W.. 1982. Principles and practice of recombinant DNA research with yeast, p. 607–636. In Strathern J. N., Jones E. W., and Broach J. R. (ed.), The molecular biology of the yeast Saccharomyces: metabolism and gene expression. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Botstein, D., Falco S. C., Stewart S. E., Brennan M., Scherer S., Stinchcomb D. T., Struhl K., and Davis R. W.. 1979. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8:17–24.
  • Broach, J. R., Atkins J. F., McGill C., and Chow L.. 1979. Identification and mapping of the transcriptional and translational products of the yeast plasmid 2μ circle. Cell 16:827–839.
  • Chaleff, D. T., and Fink G. R.. 1980. Genetic events associated with an insertion mutation in yeast. Cell 21:227–237.
  • Copeland, N. G., Hutchinson K. W., and Jenkins N. A.. 1983. Excision of the DBA ecotropic provirus in dilute coat-color revertants of mice occurs by homologous recombination involving the viral LTRs. Cell 33:379–387.
  • Delaney A. D. 1982. A DNA sequence handling program. Nucleic Acids Res. 10:61–67.
  • Dretzen, G. M., Bel lard M., Sassone-Corsi P., and Chambon P.. 1981. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal. Biochem. 112:295–298.
  • Dubois, E., Jacobs E., and Jauniaux F.-C.. 1982. Expression of the ROAM mutations in Saccharomyces cerevisiae: involvement of frans-acting regulatory elements and relation with the Tyl transcription. EMBO J. 1:1133–1139.
  • Elder, R. T., St. John T. P., Stinchcomb D. T., and Davis R. W.. 1980. Studies on the transposable element Tyl of yeast. I. RNA homologous to Tyl. Cold Spring Harbor Symp. Quant. Biol. 45:581–584.
  • Farabaugh, P. J., and Fink G. R.. 1980. Insertion of the eukaryotic transposable element Tyl creates a 5-base pair duplication. Nature (London) 286:352–356.
  • Federoff, N. V. 1983. Controlling elements in maize, p. 1–63. In Shapiro J. A. (ed.), Mobile genetic elements. Academic Press, Inc., New York.
  • Gamier, J., Osguthorpe D. J., and Robson B.. 1979. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. J. Mol. Biol. 120:97–120.
  • Ghosh, P. K., Reddy V. B., Pratak M., Lebowitz P., and Weismann S. M.. 1980. Determination of RNA sequences by primer directed synthesis and sequencing of their cDNA transcripts. Methods Enzymol. 65:580–595.
  • Gilbert, W., and Muller-Hill B.. 1970. The lactose repressor, p. 93–109. In Beckwith J. R. and Zipser D. (ed.), The lactose operon. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Haber, J. E. 1983. Mating-type genes of Saccharomyces cere-visiae, p. 560–619. In Shapiro J. A. (ed.), Mobile genetic elements. Academic Press, Inc., New York.
  • Hanahan, D. 1983. Studies on transformation of E. coli with plasmids. J. Mol. Biol. 166:557–580.
  • Hanahan, D., and Meselson M.. 1980. Plasmid screening at high colony density. Gene 10:63–68.
  • Henikoff, S. 1984. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359.
  • Hinnen, A., Hicks J. B., and Fink G. R.. 1978. Transformation of yeast. Proc. Natl. Acad. Sci. U.S.A. 75:1929–1933.
  • Jackson, I. J. 1984. Transposable elements and suppressor genes. Nature (London) 309:751–752.
  • Jenkins, N. A., Copeland N. G., Taylor B. A., and Lee B. K.. 1981. Dilute (d) coat colour mutation of DBA/2J mice is associated with the site of integration of an ecotropic MuLV genome. Nature (London) 293:370–374.
  • Laughon, A., and Scott M. P.. 1984. Sequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteins. Nature (London) 310:25–31.
  • Maniatis, T., Fritsch E. F., and Sam brook J.. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • McBride, L. J., and Camthers M. H.. 1983. An investigation of several deoxynucleoside phosphoramidites useful for synthesizing deoxyoligonucleotides. Tetrahedron Lett. 24:245–248.
  • McKay, D. B., and Steitz T. A.. 1981. Structure of catabolite gene activator protein at 2.9 A resolution suggests binding to left-handed DNA. Nature (London) 290:744–749.
  • Messing, J. 1983. New M13 vectors for cloning. Methods Enzymol. 101:20–78.
  • Modolell, J., Bender W., and Meselson M.. 1983. Drosophila melanogaster mutations suppressible by the suppressor of hairy-wing are insertions of a 7.3-kilobase mobile element. Proc. Natl. Acad. Sci. U.S.A. 80:1678–1682.
  • Nasmyth, K. A. 1982. Molecular genetics of yeast mating type. Annu. Rev. Genet. 16:439–500.
  • Ohlendorf, D. H., Anderson W. F., and Matthews B. W.. 1983. Many gene-regulatory proteins appear to have a similar α-helical fold that binds DNA and evolved from a common precursor. J. Mol. Evol. 19:109–114.
  • Orr-Weaver, T. L., Szostak J. W., and Rothstein R. J.. 1981. Yeast transformation: a model system for the study of recombination. Proc. Natl. Acad. Sci. U.S.A. 78:6354–6358.
  • Pabo, C. O., and Lewis M.. 1982. The operator-binding domain of λ repressor: structure and DNA recognition. Nature (London) 298:443–447.
  • Pikielny, C. W., Teem J. L., and Rosbash M.. 1983. Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: implications for Ul RNA and metazoan mRNA splicing. Cell 34:395–403.
  • Roeder, G. S., Farabaugh P. J., Chaleff D. T., and Fink G. R.. 1980. The origins of gene instability in yeast. Science 209:1375–1380.
  • Roeder, G. S., and Fink G. R.. 1980. DNA rearrangements associated with a transposable element in yeast. Cell 21:239–249.
  • Roeder, G. S., and Fink G. R.. 1982. Movement of yeast transposable elements by gene conversion. Proc. Natl. Acad. Sci. U.S.A. 79:5621–5625.
  • Roeder, G. S., and Fink G. R.. 1983. Transposable elements in yeast, p. 299–328. In Shapiro J. A. (ed.), Mobile genetic elements. Academic Press, Inc., New York.
  • Sanger, F., Coulson A. R., Barrell B. G., Smith A. J. H., and Roe B. A.. 1980. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J. Mol. Biol. 143:161–178.
  • Sanger, F., Nicklen S., and Coulson A. R.. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. U.S.A. 74:5463–5467.
  • Sauer, R. T., Yocum R. R., Doolittle R. F., Lewis M., and Pabo C. O.. 1982. Homology among DNA-binding proteins suggests use of a conserved super-secondary structure. Nature (London) 298:447–451.
  • Shepherd, J. C. W., McGinnis W., Carrasco A. E., DeRobertis E. M., and Gehring W.. Fly and frog homoeo domains show homologies with yeast mating type regulatory proteins. Nature (London) 310:70–71.
  • Sherman, F., Fink G. R., and Lawrence C. W.. 1983. Methods in yeast genetics: laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Silverman, S. J., and Fink G. R.. 1984. Effects of Ty insertions on HIS4 transcription in Saccharomyces cerevisiae. Mol. Cell. Biol. 4:1246–1251.
  • Simchen, G., Winston F., Styles C. A., and Fink G. R.. 1984. Ty-mediated gene expression of the LYS2 and HIS4 genes of Saccharomyces cerevisiae is controlled by the same SPT genes. Proc. Natl. Acad. Sci. U.S.A. 81:2431–2434.
  • Smith, M., Leung D. W., Gillam S., Astell C. R., Montgomery D. L., and Hall B. H.. 1979. Sequence of the gene for iso-l-cytochrome c in Saccharomyces cerevisiae. Cell 16:753–761.
  • Steitz, T. A., Ohlendorf D. H., McKay D. B., Anderson W. F., and Matthews B. W.. 1982. Structural similarity in the DNA-binding domains of catabolite gene activator and cro repressor proteins. Proc. Natl. Acad. Sci. U.S.A. 79:3097–3100.
  • Sweet, H. O. 1983. Dilute suppressor, a new suppressor gene in the house mouse. J. Hered. 74:305–306.
  • Taguchi, A. K. W., Ciriacy M., and Young E. T.. 1984. Carbon source dependence of transposable element-associated gene activation in Saccharomyces cerevisiae. Mol. Cell. Biol. 4:61–68.
  • Takeda, Y., Ohlendorf D. H., Anderson W. F., and Matthews B. W.. 1983. DNA-binding proteins. Science 221:1020–1026.
  • Thomas, P. S. 1980. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. U.S.A. 77:5201–5205.
  • Viera, J., and Messing J.. 1982. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268.
  • Winston, F., Chaleff D. T., Valent B., and Fink G. R.. 1984. Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107:179–197.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.