Disruption of the Three Cytoskeletal Networks in Mammalian Cells Does Not Affect Transcription, Translation, or Protein Translocation Changes Induced by Heat Shock

LITERATURE CITED

• Arrigo, A. P., Fakan S., and Tissieres A.. 1980. Localization of the heat shock-induced proteins in Drosophila melanogaster tissue culture cells. Dev. Biol. 78:86–103.
   PubMed Web of Science ®Google Scholar
• Ashburner, M., and Bonner J. J.. 1979. The induction of gene activity in Drosophila by heat shock (review). Cell 17:241–254.
   PubMed Web of Science ®Google Scholar
• Biessmann, H., Falkner F. G., Saumweber H., and Walter M. F.. 1982. Disruption of the vimentin cytoskeleton may play a role in heat shock response, p. 275–281. In Schlesinger M. J., Ashburner M., and Tissieres A. (ed.), Heat shock: from bacteria to man. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
   Google Scholar
• Blattler, D. P., Garner F., Van Slyke K., and Bradley A.. 1972. Quantitative electrophoresis on polyacrylamide gels of 2-40%. J. Chromatogr. 64:147–155.
   Web of Science ®Google Scholar
• Blose, S. H., Meltzer D. I., and Feramisco J. R.. 1984. lOnm filaments are induced to collapse in living cells microinjected with monoclonal and polyclonal antibodies against tubulin. J. Cell Biol. 98:847–858.
   PubMed Web of Science ®Google Scholar
• Bonner, W. M., and Laskey R. A.. 1975. A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem. 46:83–88.
   Google Scholar
• Brady, S. T., and Lasek R. J.. 1982. Axonal transport: a cell biological method for studying proteins that associate with the cytoskeleton. Methods Cell Biol. 25:365–398.
   PubMedGoogle Scholar
• Cerveza, M., Dreyfuss G., and Penman S.. 1981. Messenger RNA is translated when associated with the cytoskeletal framework in normal and VSV-infected Hela cells. Cell 23:113–120.
   PubMedGoogle Scholar
• Collot, M., Louvard D., and Singer S. J.. 1984. Lysosomes are associated with microtubules and not with intermediate filaments in cultured fibroblasts. Proc. Natl. Acad. Sci. U.S.A. 81:788–792.
   PubMedGoogle Scholar
• DiDomenico, B. J., Bugaisky G. E., and Lindquist S.. 1982. The heat shock response is self-regulated at both the transcriptional and post-transcriptional levels. Cell 31:593–603.
   PubMed Web of Science ®Google Scholar
• Feramisco, J. R., and Blose S. H.. 1980. The distribution of fluorescently labelled alpha-actinin after microinjection into living fibroblasts in relation to other structural proteins. J. Cell Biol. 86:608–614.
   PubMedGoogle Scholar
• Freed, J. J., and Lebowitz M. M.. 1970. The association of a class of saltatory movements with microtubules in cultured cells. J. Cell Biol. 45:334–354.
   PubMedGoogle Scholar
• Fulton, A., Wan K. A., and Penman S.. 1980. The spatial distribution of polyribosomes in 3TC cells and the associated assembly of proteins into the skeletal framework. Cell 20: 849–857.
   PubMedGoogle Scholar
• Goldman, R. D., Milstead A., Schloss J. A., Starger J., and Yerna M. J.. 1979. Cytoplasmic fibers in mammalian cells: cytoskeletal and contractile elements. Annu. Rev. Physiol. 41:703–722.
   PubMedGoogle Scholar
• Goldman, R. D., Pollard T., and Rosenbaum J. (ed.). 1976. Cell Motility. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
   Google Scholar
• Hickey, E., and Weber L. A.. 1982. Preferential translation of heat-shock mRNA's in HeLa cells, p. 199–206. In Schlesinger M. J., Ashburner M., and Tissieres A. (ed.), Heat shock: from bacteria to man. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
   Google Scholar
• Krippl, B., Ferguson B., Rosenberg M., and Westphal H.. 1984. Functions of purified E1A protein microinjected into mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 81:6988–6993.
   PubMedGoogle Scholar
• Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685.
   PubMed Web of Science ®Google Scholar
• Lee, P. C, Bochner B. R., and Ames B. N.. 1983. AppppA, heat shock stress, and cell oxidation. Proc. Natl. Acad. Sci. U.S.A. 80:7496–7500.
   PubMed Web of Science ®Google Scholar
• Lenk, R., and Penman S.. 1979. The cytoskeletal framework and poliovirus metabolism. Cell 16:289–301.
   PubMed Web of Science ®Google Scholar
• Lenk, R., Ransom L., Kaufmann Y., and Penman S.. 1977. A cytoskeletal structure with associated polyribosomes obtained from HeLa cells. Cell 10:67–78.
   PubMed Web of Science ®Google Scholar
• McKenzie, S. L., and Messelson M.. 1977. Translation in vitro of Drosophila heat shock messages. J. Mol. Biol. 117:279–283.
   PubMed Web of Science ®Google Scholar
• Mose-Larsen, P., Bravo R., Fey S. J., Small J. V., and Celis J. E.. 1983. Putative association of mitochondria with a subpopulation of intermediate-sized filaments in cultured human skin fibroblasts. Cell 31:681–692.
   Google Scholar
• Parker, C. S., and Topol J.. 1984. A Drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene. Cell 37:273–283.
   PubMed Web of Science ®Google Scholar
• Rogalski, A. A., Bergmann J. E., and Singer S. J.. 1982. Intracellular transport and processing of an integral membrane protein is independent of the assembly status of the cytoplasmic microtubules. J. Cell Biol. 95:377a.
   Google Scholar
• Schlesinger, M. J., Ashburner M., and Tissieres A. (ed.). 1982. Heat shock: from bacteria to man. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
   Google Scholar
• Scott, M., and Pardue M. L.. 1981. Translational control in lysates of Drosophila melanogaster cells. Proc. Natl. Acad. Sci. U.S.A. 78:3353–3357.
   PubMed Web of Science ®Google Scholar
• Spradling, A., Pardue M. L., and Penman S.. 1979. Messenger RNA in heat-shocked Drosophila cells. J. Mol. Biol. 109: 559–587.
   Google Scholar
• Storti, R. V., Scott M. P., Rich A., and Pardue M. L.. 1980. Translational control of protein synthesis in response to heat shock in D. melanogaster cells. Cell 22:825–834.
   PubMed Web of Science ®Google Scholar
• Thomas, G. P., and Mathews M. B.. 1984. Alterations of transcription and translation in HeLa cells exposed to amino acid analogs. Mol. Cell. Biol. 4:1063–1072.
   PubMed Web of Science ®Google Scholar
• Thomas, G. P., Welch W. J., Mathews M. B., and Feramisco J. R.. 1982. Molecular and cellular effects of heat shock and related treatments of mammalian tissue culture cells. Cold Spring Harbor Symp. Quant. Biol. 46:985–996.
   PubMedGoogle Scholar
• Velazquez, J. M., DiDomenico B. J., and Lindquist S.. 1980. Intracellular localization of heat shock proteins in Drosophila. Cell 20:679–689.
   PubMed Web of Science ®Google Scholar
• von Venrooij, W. J., Sillekens P. T. G., von Eekelen C. A. G., and Reinders R. J.. 1981. On the association of mRNA with the cytoskeleton in uninfected and adenovirus infected human KB cells. Exp. Cell Res. 135:79–91.
   PubMedGoogle Scholar
• Wang, K., Feramisco J. R., and Ash J. F.. 1982. Fluorescent localization of contractile proteins in tissue culture cells. Methods Enzymol. 85:514–530.
   PubMed Web of Science ®Google Scholar
• Welch, W. J., and Feramisco J. R.. 1984. Nuclear and nucleolar localization of the 72,000 dalton heat shock protein in heat-shocked mammalian cells. J. Biol. Chem. 259:4501–4513.
   PubMed Web of Science ®Google Scholar
• Welch, W. J., and Feramisco J. R.. 1985. Rapid purification of mammalian 70,000-dalton stress proteins: affinity of the proteins for nucleotides. Mol. Cell. Biol. 5:1228–1237.
   Google Scholar
• Welch, W. J., Garrels J., Thomas G. P., Lin J. J.-C., and Feramisco J. R.. 1983. Biochemical characterization of the mammalian stress proteins and identification of two stress proteins as glucose- and Ca++-ionophore-regulated proteins. J. Biol. Chem. 258:7102–7111.
   PubMed Web of Science ®Google Scholar
• Wilson, L. (ed.). 1982. Methods in cell biology, vol. 24 and 25. Academic Press, Inc., New York.
   Google Scholar
• Zumbe, A., Stahli C., and Traeschel H.. 1982. Association of an Mr 50,000 cap-binding protein with the cytoskeleton in BHK cells. Proc. Natl. Acad. Sci. U.S.A. 79:2927–2931.
   PubMedGoogle Scholar