4
Views
2
CrossRef citations to date
0
Altmetric
Research Article

Interaction of the FLP Recombinase of the Saccharomyces cerevisiae 2 µm Plasmid with Mutated Target Sequences

, , &
Pages 2482-2489 | Received 15 Jan 1986, Accepted 10 Apr 1986, Published online: 31 Mar 2023

LITERATURE CITED

  • Abremski, K., and R. Hoess. 1985. Phage PI cre-loxP site-specific recombination. Effects of DNA supercoiling on catenation and knotting of recombinant products. J. Mol. Biol. 184:211–220.
  • Andrews, B. J., G. A. Proteau, L. G. Beatty, and P. D. Sadowski. 1985. The FLP recombinase of the 2-micron circle DNA of yeast: interaction with its target sequences. Cell 40:795–803.
  • Babineau, D., D. Vetter, B. J. Andrews, R. M. Gronostajski, G. A. Proteau, L. G. Beatty, and P. D. Sadowski. 1985. The FLP protein of the 2-micron plasmid of yeast. Purification of the protein from Escherichia coli cells expressing the cloned FLP gene. J. Biol. Chem. 260:12313–12319.
  • Bauer, C. E., S. D. Hesse, J. F. Gardner, and R. I. Gumport. 1984. DNA interactions during bacteriophage λ site-specific recombination. Cold Spring Harbor Symp. Quant. Biol. 49:699–705.
  • Broach, J. R., V. R. Guarascio, and M. Jayaram. 1982. Recombination within the yeast plasmid 2-micron circle is site-specific. Cell 29:227–234.
  • Cox, M. M. 1983. The FLP protein of the yeast 2-micron plasmid: expression of a eucaryotic genetic recombination systern in E. coli. Proc. Natl. Acad. Sci. USA 80:4223–4227.
  • DeMassy, B., F. W. Studier, L. Dorgai, E. Applebaum, and R. A. Weisberg. 1984. Enzymes and site of genetic recombination: studies with gene-3 endonuclease of phage T7 and with site-affinity mutants of phage X. Cold Spring Harbor Symp. Quant. Biol. 49:715–726.
  • Gronostajski, R. M., and P. D. Sadowski. 1985. Determination of DNA sequences essential for FLP-mediated recombination by a novel method. J. Biol. Chem. 260:12320–12327.
  • Gronostajski, R. M., and P. D. Sadowski. 1985. The FLP protein of the 2-micron plasmid of yeast. Inter- and intramolecular reactions. J. Biol. Chem. 260:12328–12335.
  • Gronostajski, R. M., and P. D. Sadowski. 1985. The FLP recombinase of the Saccharomyces cerevisiae 2μm plasmid attaches covalently to DNA via a phosphotyrosyl linkage. Mol. Cell. Biol. 5:3274–3279.
  • Hartley, J. L., and J. E. Donelson. 1980. Nucleotide sequence of the yeast plasmid. Nature (London) 286:860–865.
  • Jayaram, M. 1985. Two-micrometer circle site-specific recombination: the minimal substrate and the possible role of flanking sequences. Proc. Natl. Acad. Sci. USA 82:5875–5879.
  • Johnson, R. C., and M. I. Simon. 1985. Hin-mediated site-specific recombination requires two 26 bp recombination sites and a 60 bp recombinational enhancer. Cell 41:784–791.
  • Maxam, A. M., and W. Gilbert. 1980. Sequencing end-labelled DNA with base-specific chemical clevages. Methods Enzymol. 65:449–560.
  • McLeod, M., F. Volkert, and J. Broach. 1984. Components of the site-specific recombination system encoded by the yeast plasmid 2-micron circle. Cold Spring Harbor Symp. Quant. Biol. 49:779–787.
  • Meyer-Leon, L., J. F. Senecoff, R. C. Bruckner, and M. M. Cox. 1984. Site-specific recombination promoted by the FLP protein of the yeast 2-micron plasmid in vitro. Cold Spring Harbor Symp. Quant. Biol. 49:797–804.
  • Mizuuchi, K., R. Weisberg, L. Enquist, M. Mizuuchi, M. Buraczynska, C. Foeller, P.-L. Hsu, W. Ross, and A. Landy. 1980. Structure and function of the phage λ att site: size, int-binding sites and location of the crossover point. Cold Spring Harbor Symp. Quant. Biol. 45:429–437.
  • Riggs, A. D., H. Suzuki, and S. Bourgeois. 1970. lac repressor-operator interaction. I. Equilibrium studies. J. Mol. Biol. 48:67–83.
  • Sadowski, P. D., D. D. Lee, B. J. Andrews, D. Babineau, L. Beatty, M. J. Morse, G. Proteau, and D. Vetter. 1984. In vitro systems for genetic recombination of the DNAs of bacteriophage T7 and of yeast 2-micron circle. Cold Spring Harbor Symp. Quant. Biol. 49:789–796.
  • Senecoff, J. F., R. C. Bruckner, and M. M. Cox. 1985. The FLP recombinase of the yeast 2-micron plasmid: characterization of its recombination site. Proc. Natl. Acad. Sci. USA 82:7270–7274.
  • Vetter, D., B. J. Andrews, L. Roberts-Beatty, and P. D. Sadowski. 1983. Site-specific recombination of yeast 2-micron DNA in vitro. Proc. Natl. Acad. Sci. USA 80:7281–7288.
  • Vieira, J., and J. Messing. 1982. The pUC plasmids, an M13 mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268.
  • Weisberg, R. A., L. W. Enquist, C. Foeller, and A. Landy. 1983. Role for DNA homology in site-specific recombination. The isolation and characterization of a site affinity mutant of coli-phage λ. J. Mol. Biol. 170:318–342.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.