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Research Article

Effect of Intron Mutations on Processing and Function of Saccharomyces cerevisiaeSUP53 tRNA In Vitro and In Vivo

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Pages 2663-2673 | Received 04 Dec 1985, Accepted 02 Apr 1986, Published online: 31 Mar 2023

LITERATURE CITED

  • Andreadis, A., Y.-P. Hsu, G. B. Kohlhaw, and P. Schimmel. 1982. Nucleotide sequence of yeast LEU2 shows 5′-non-coding region has sequences cognate to leucine. Cell 31:319–325.
  • Baldi, M. L., E. Mattoccia, and G. P. Tocchini-Valentini. 1983. Role of RNA structure in splicing: excision of the intervening sequence in yeast tRNALeu3 is dependent on the formation of a D stem. Cell 35:109–115.
  • Birnboim, H. C., and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513–1523.
  • Bjork, G. R. 1984. Modified nucleosides in RNA—their formation and function, p. 291–330. In D. Apirion (ed.), Processing of RNA. CRC Press, Boca Raton, Fla.
  • Bolivar, F., and K. Backman. 1979. Plasmids of Escherichia coli as cloning vectors. Methods Enzymol. 68:245–267.
  • Brown, A., C.-J. Pan, and W. F. Marzluff. 1982. Methylation of ribonucleic acid in a cell-free system from mouse myeloma cells. Biochemistry 21:4303–4310.
  • Cech, T. R. 1983. RNA splicing: three themes with variations. Cell 34:713–716.
  • Colby, D. S., P. Schedl, and C. Guthrie. 1976. A functional requirement for modification of the wobble nucleotide in the anticodon of a suppressor tRNA. Cell 9:449–463.
  • Engelke, D. R., P. Gegenheimer, and J. Abelson. 1985. Nucleolytic processing of a tRNAArg-tRNAAsp dimeric precursor by a homologous component from Saccharomyces cerevisiae. J. Biol. Chem. 260:1271–1279.
  • Fischoff, D. A., R. H. Waterston, and M. V. Olson. 1984. The yeast cloning vector YEpl3 contains a tRNALeu3 gene that can mutate to an amber suppressor. Gene 27:239–251.
  • Gamulin, V., J. Mao, B. Appel, M. Sumner-Smith, F. Yamao, and D. Soll. 1983. Six Schizosaccharomyces pombe tRNA genes including a gene for a tRNALys with an intervening sequence which cannot base-pair with the anticodon. Nucleic Acids Res. 11:8537–8546.
  • Gefter, M. L., and R. L. Russell. 1969. Role of modifications in tyrosine transfer RNA: a modified base affecting ribosome binding. J. Mol. Biol. 39:145–157.
  • Green, C. J., H. O. Kammen, and E. E. Penhoet. 1982. Purification and properties of a mammalian tRNA pseudouridine synthase. J. Biol. Chem. 257:3045–3052.
  • Greer, C. L., and J. A. Abelson. 1984. RNA splicing: rearrangement of RNA sequences in the expression of split genes. Trends Biochem. Sci. 4:139–141.
  • Greer, C. L., B. Javor, and J. Abelson. 1983. RNA ligase in bacteria: formation of a 2′,5′ linkage by an E. coli extract. Cell 33:899–906.
  • Greer, C. L., C. L. Peebles, P. Gegenheimer, and J. Abelson. 1983. Mechanism of action of a yeast RNA ligase in tRNA splicing. Celi 32:537–546.
  • Guthrie, C., J. G. Seidman, S. Altman, B. G. Barrell, J. D. Smith, and W. H. McClain. 1973. Identification of tRNA precursor molecules made by phage T4. Nature (London) New Biol. 246:6–11.
  • Hirsh, D. 1971. Tryptophan transfer RNA as the UGA suppressor. J. Mol. Biol. 58:439–458.
  • Ito, H., Y. Fukada, K. Murata, and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163–168.
  • Janner, F., G. Vogeli, and R. Fluri. 1980. The antisuppressor strain sin-1 of Schizosaccharomyces pombe lacks the modification isopentenyladenosine in transfer RNA. J. Mol. Biol. 139:207–219.
  • Johnson, J. D., R. Ogden, P. Johnson, J. Abelson, P. Dembeck, and K. Itakura. 1980. Transcription and processing a yeast tRNA gene containing a modified intervening sequence. Proc. Natl. Acad. Sci. USA 77:2564–2568.
  • Johnson, P. F., and J. Abelson. 1983. The yeast tRNATyr gene intron is essential for correct modification of its tRNA product. Nature (London) 303:681–687.
  • Kang, H. S., R. C. Ogden, G. Knapp, C. L. Peebles, and J. Abelson. 1979. Structure of yeast tRNA precursors containing intervening sequences, p. 69–84. In R. Axel, T. Maniatis, and F. Fox (ed.), Eukaryotic gene regulation. Academic Press, Inc., New York.
  • Keith, J. M., E. M. Winters, and B. Moss. 1980. Purification and characterization of a HeLa cell transfer RNA (cytosine-5-)- methyltransferase. J. Biol. Chem. 255:4636–4644.
  • Kim, S.-H. 1979. Crystal structure of yeast tRNAPhe and general structural features of other tRNAs, p. 83–100. In P. R. Schimmel, D. Soll, and J. N. Abelson (ed.), Transfer RNA: structure, properties, and recognition. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Koski, R. A., S. G. Clarkson, J. Kurjan, B. D. Hall, and M. Smith. 1980. Mutations of the yeast SUP4 tRNATyr locus: transcription of the mutant genes in vitro. Cell 22:415–425.
  • Koski, R. A., M. Worthington, D. S. Allison, and B. D. Hall. 1982. An in vitro RNA polymerase III system from S. cerevisiae: effects of deletions and point mutations upon SUP4 gene transcription. Nucleic Acids Res. 10:8127–8143.
  • Kurjan, J., B. D. Hall, S. Gillam, and M. Smith. 1980. Mutations at the yeast SUP4 tRNATyr locus: DNA sequence changes in mutants lacking suppressor activity. Cell 20:701–709.
  • Laten, J., J. Gorman, and R. M. Bock. 1980. Isopentenyladenosine deficient tRNA from an antisuppressor mutant of Saccharomyces cerevisiae. Nucleic Acids Res. 5:4329–4342.
  • Lee, M.-C., and G. Knapp. 1985. Transfer RNA splicing in Saccharomyces cerevisiae: secondary and tertiary structures of the substrates. J. Biol. Chem. 260:3108–3115.
  • Liebman, S. W., Z. Srodulski, C. R. Reed, J. W. Stewart, F. Sherman, and G. Brennan. 1984. Yeast amber suppressors corresponding to tRNALeu3 genes. J. Mol. Biol. 178:209–226.
  • Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Mattocia, E., M. I. Baldi, G. Pande, R. Ogden, and G. Tocchini-Valentini. 1983. Mutations in the A block of the yeast tRNALeu3 gene that allows transcription but abolishes splicing and 5′ end maturation. Cell 32:67–76.
  • McClain, W. H., and J. G. Seidman. 1975. Genetic perturbations that reveal tertiary conformation of tRNA precursor molecules. Nature (London) 257:106–110.
  • Melton, D. A., E. M. DeRobertis, and R. Cortese. 1980. Order and intracellular location of the events involved in maturation of a spliced tRNA. Nature (London) 284:143–148.
  • Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Miller, J. H., and A. M. Albertini. 1983. Effects of surrounding sequence on the suppression of nonsense codons. J. Mol. Biol. 164:59–71.
  • Mitra, S. K., F. Lustig, B. Akesson, U. Lagerkvist, and L. Strid. 1977. Codon-anticodon recognition in the valine codon family. J. Biol. Chem. 252:471–478.
  • Newman, A. J., R. C. Ogden, and J. Abelson. 1983. tRNA gene transcription in yeast: effects of specified base substitutions in the intragenic promoter. Cell 35:117–125.
  • Ng, R., H. Domdey, G. Larson, J. J. Rossi, and J. Abelson. 1985. A test for intron function in the yeast actin gene. Nature (London) 314:183–184.
  • Nishikura, K., and E. M. DeRobertis. 1981. RNA processing in microinjected Xenopus oocytes. Sequential addition of base modifications in a spliced transfer RNA. J. Mol. Biol. 145:405–420.
  • Nishikura, K., J. Kurjan, B. D. Hall, and E. M. DeRobertis. 1982. Genetic analysis of the processing of a spliced tRNA. EMBO J. 1:263–268.
  • Ogden, R. C., M.-C. Lee, and G. Knapp. 1984. Transfer RNA splicing in Saccharomyces cerevisiae: defining the substrates. Nucleic Acids Res. 12:9367–9382.
  • Pearson, D., I. Willis, H. Hottinger, J. Bell, A. Kumar, U. Leupold, and D. Soll. 1985. Mutations preventing expression of sup3 tRNASer nonsense suppressors of Schizosaccharomyces pombe. Mol. Cell. Biol. 5:808–815.
  • Peebles, C. L., P. Gegenheimer, and J. Abelson. 1983. Precise excision of intervening sequences from precursor tRNAs by a membrane-associated yeast endonuclease. Cell 32:525–536.
  • Phizicky, E., R. Schwartz, and J. Abelson. 1986. S. cerevisiae tRNA ligase: purification of the protein and isolation of the structural gene. J. Biol. Chem. 261:2978–2986.
  • Raymond, G., and J. D. Johnson. 1983. The role of non-coding DNA sequences in transcription and processing of a yeast tRNA. Nucleic Acids Res. 11:5969–5988.
  • Rothstein, R. J. 1983. One-step gene disruption in yeast. Methods Enzymol. 101:202–212.
  • Sakano, H., Y. Shimura, and H. Ozeki. 1974. Selective modification of nucleosides of tRNA precursors accumulated in a temperature-sensitive mutant of Escherichia coli. FEBS Lett. 48:117–121.
  • Saneyoshi, M., Z. Ohashi, F. Harada, and S. Nishimura. 1972. Isolation and characterization of 2-methyladenosine from Escherichia coli tRNAGlu2, tRNAAsp1, tRNAHis1 and tRNAArg. Biochim. Biophys. Acta 262:1–10.
  • Sanger, F., S. Nicklin, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463–5467.
  • Sharp, P. 1985. On the origin of RNA splicing and introns. Cell 42:397–400.
  • Shaw, K. J., and M. V. Olson. 1984. Effects of altered 5′- flanking sequences on the in vivo expression of a Saccharomyces cerevisiae tRNATyr gene. Mol. Cell. Biol. 4:657–665.
  • Sherman, F., G. R. Fink, and J. B. Hicks. 1983. Methods in yeast genetics: laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Sherman, F., B. Ono, and J. R. Stewart. 1979. Use of the iso-l-cytochrome C system for investigating nonsense mutants and suppressors in yeast, p. 133–153. In J. E. Celis and J. D. Smith (ed.), Nonsense mutations and tRNA suppressors. Academic Press, Inc., London.
  • Sprinzl, M., J. Moll, F. Meissner, and T. Hartman. 1985. Compilation of tRNA sequences. Nucleic Acids Res. 13:r1–r49.
  • Sumner-Smith, M., H. Hottinger, I. Willis, T. L. Koch, R. Arentzen, and D. Soll. 1984. The sup8 tRNALeu gene of Schizosaccharomyces pombe has an unusual intervening sequence and reduced pairing in the anticodon stem. Mol. Gen. Genet. 197:447–452.
  • Swerdlow, H., and C. Guthrie. 1984. Structures of introncontaining tRNA precursors: analysis of solution conformation using chemical and enzymatic probes. J. Biol. Chem. 259:5197–5207.
  • Weissenbach, J., and G. Dirheimer. 1978. Pairing properties of the methylester of 5-carboxymethyl uridine in the wobble position of the yeast tRNAArg3. Biochim. Biophys. Acta 518:530–534.
  • Willis, L., H. Hottinger, D. Pearson, V. Chisholm, U. Leupold, and D. Soll. 1984. Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor. EMBO J. 3:1573–1580.

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