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Research Article

Phosphorylation of the Saccharomyces cerevisiae Equivalent of Ribosomal Protein S6 Has No Detectable Effect on Growth

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Pages 1338-1345 | Received 25 Aug 1986, Accepted 18 Dec 1986, Published online: 31 Mar 2023

LITERATURE CITED

  • Abovich, N., and M. Rosbash. 1984. Two genes for ribosomal protein 51 of Saccharomyces cerevisiae complement and contribute to the ribosomes. Mol. Cell. Biol. 4:1871–1879.
  • Ballinger, D. G., and T. Hunt. 1981. Fertilization of sea urchin eggs is accompanied by 40S ribosomal subunit phosphorylation. Dev. Biol. 87:277–285.
  • Blenis, J., and R. L. Erikson. 1985. Regulation of a ribosomal protein S6 kinase activity by the Rous sarcoma virus transforming protein, serum, or phorbol ester. Proc. Natl. Acad. Sci. USA 82:7621–7625.
  • Blenis, J., and R. L. Erikson. 1986. Stimulation of ribosomal protein S6 kinase activity by pp60v-src or by serum: dissociation from phorbol ester-stimulated activity. Proc. Natl. Acad. Sci. USA 83:1733–1737.
  • Boeke, J. D., F. LaCroute, and G. R. Fink. 1984. A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol. Gen. Genet. 197:345–346.
  • Burkhard, S. J., and J. A. Traugh. 1983. Changes in ribosome function by cAMP-dependent and cAMP-independent phosphorylation of ribosomal protein S6. J. Biol. Chem. 258:14003–14008.
  • Decker, S. 1981. Phosphorylation of ribosomal protein S6 in avian sarcoma virus-transformed chicken embryo fibroblasts. Proc. Natl. Acad. Sci. USA 78:4112–4115.
  • Duncan, R., and E. H. McConkey. 1982. Preferential utilization of phosphorylated 40S ribosomal subunits during initiation complex formation. Eur. J. Biochem. 123:535–538.
  • Elion, E., and J. R. Warner. 1986. An RNA polymerase I enhancer in Saccharomyces cerevisiae. Mol. Cell. Biol. 6:2089–2097.
  • Erikson, E., and J. L. Mailer. 1985. A protein kinase from Xenopus eggs specific for ribosomal protein S6. Proc. Natl. Acad. Sci. USA 82:742–746.
  • Fry, M. J., A. Gebhardt, P. J. Parker, and J. G. Foulkes. 1985. Phosphotidylinositol turnover and transformation of cells by Abelson murine leukemia virus. EMBO J. 4:3173–3178.
  • Gabrielli, B., R. E. H Wettenhall, B. E. Kemp, M. Quinn, and L. Bizonova. 1984. Phosphorylation of ribosomal protein S6 and a peptide analogue of S6 by a protease-activated kinase isolated from rat liver. FEBS Lett. 175:219–226.
  • Glover, C. V. C. 1982. Heat shock induces rapid dephosphorylation of a ribosomal protein in Drosophila. Proc. Natl. Acad. Sci. USA 79:1781–1785.
  • Gordon, J., P. J. Nielsen, K. L. Manchester, H. Towbin, L. Jimenez de Asua, and G. Thomas. 1982. Criteria for establishment of the biological significance of ribosomal protein phosphorylation. Curr. Top. Cell. Reg. 21:89–99.
  • Ito, H., Y. Fukuda, K. Murata, and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163–168.
  • Jakubowicz, T. 1985. Phosphorylation-dephosphorylation changes in yeast ribosomal proteins S2 and S6 during growth under normal and hyperthermal conditions. Acta Biochim. Polonica 32:7–12.
  • Juliani, M. H., J. C. Maia, and M. C. Bonato. 1983. Increased phosphorylation of a ribosomal protein during aggregation of the slime mold Dictyostelium discoideum. FEBS Lett. 154:400–406.
  • Kristiansen, K., and A. Kruger. 1978. Ribosomal proteins in growing and starved Tetrahymena pyriformis: starvationinduced phosphorylation of ribosomal proteins. Biochim. Biophys. Acta 521:434–451.
  • Kruse, C., S. P. Johnson, and J. R. Warner. 1985. Phosphorylation of the yeast equivalent of ribosomal protein S6 is not essential for growth. Proc. Natl. Acad. Sci. USA 82:7515–7519.
  • Leader, D. P., A. Thomas, and H. O. Voorma. 1981. The protein synthetic activity in vitro of ribosomes differing in the extent of phosphorylation of their ribosomal proteins. Biochim. Biophys. Acta 656:69–75.
  • Leer, R. J., M. M. C. van Raamsdonk-Duin, C. M. T. Molenaar, L. H. Cohen, W. H. Mager, and R. J. Planta. 1982. The structure of the gene coding for the phosphorylated ribosomal protein S10 in yeast. Nucleic Acids Res. 10:5869–5878.
  • Leer, R. J., M. M. C. van Raamsdonk-Duin, C. M. T. Molenaar, H. M. A. Witsenboer, W. H. Mager, and R. J. Planta. 1985. Yeast contains two functional genes coding for ribosomal protein S10. Nucleic Acids Res. 13:5027–5039.
  • Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
  • Martin-Perez, J., M. Siegmann, and G. Thomas. 1984. EGF, PGF2α and insulin induce the phosphorylation of identical S6 peptides in Swiss mouse 3T3 cells: effect of cAMP on early sites of phosphorylation. Cell 36:287–294.
  • Mastropaolo, W., and E. C. Henshaw. 1981. Phosphorylation of ribosomal protein S6 in the Ehrlich ascites tumor cell: lack of effect of phosphorylation upon ribosomal function in vitro. Biochim. Biophys. Acta 656:246–255.
  • Nielsen, P. J., G. Thomas, and J. L. Mailer. 1982. Increased phosphorylation of ribosomal protein S6 during meiotic maturation of Xenopus oocytes. Proc. Natl. Acad. Sci. USA 79:2937–2941.
  • Novak-Hofer, I., and G. Thomas. 1985. Epidermal growth factor-mediated activation of an S6 kinase in Swiss mouse 3T3 cells. J. Biol. Chem. 260:10314–10319.
  • Otaka, E., T. Kumazaki, and K. Matsumoto. 1986. In vivo phosphorylation of Saccharomyces cerevisiae ribosomal protein S10 by cyclic-AMP-dependent protein kinase. J. Bacteriol. 167:713–715.
  • Parker, P. J., M. Katan, M. D. Waterfield, and D. P. Leader. 1985. The phosphorylation of eukaryotic ribosomal protein S6 by protein kinase C. Eur. J. Biochem. 148:579–586.
  • Pearson, N. J., H. M. Fried, and J. R. Warner. 1982. Yeast use translational control to compensate for extra copies of a ribosomal protein gene. Cell 29:347–355.
  • Perisic, O., and J. A. Traugh. 1983. Protease-activated kinase II mediates multiple phosphorylation of ribosomal protein S6 in reticulocytes. J. Biol. Chem. 258:13998–14002.
  • Rothstein, R. J. 1983. One-step gene disruption in yeast. Methods Enzymol. 101:202–211.
  • Rubin, G. 1973. The nucleotide sequence of Saccharomyces cerevisiae 5.8S ribosomal RNA. J. Biol. Chem. 248:3860–3875.
  • Scharf, K.-D., and L. Nover. 1982. Heat-shock-induced alterations of ribosomal protein phosphorylation in plant cell cultures. Cell 30:427–437.
  • Smith, C. J., P. J. Wejksnora, J. R. Warner, C. S. Rubin, and O. M. Rosen. 1979. Insulin-stimulated protein phosphorylation in 3T3-L1 preadipocytes. Proc. Natl. Acad. Sci. USA 76:2725–2729.
  • Southern, E. M. 1975. Detection of specific sequences among DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. USA 77:5201–5205.
  • Szyszka, R., and E. Gasior. 1984. Phosphorylation of ribosomal proteins during differentiation of Saccharomyces cerevisiae. Acta Biochim. Polonica 4:375–382.
  • Tabarini, D., J. Heinrich, and O. M. Rosen. 1985. Activation of S6 kinase activity in 3T3-L1 cells by insulin and phorbol ester. Proc. Natl. Acad. Sci. USA 82:4369–4373.
  • Terao, K., and K. Ogata. 1979. Proteins of small subunits of rat liver ribosomes that interact with poly(U). II. Crosslinks between poly(U) and ribosomal proteins in 40S subunits induced by UV irradiation. J. Biochem. 86:605–617.
  • Thomas, G., J. Martin-Perez, M. Siegmann, and A. M. Otto. 1982. The effect of serum, EGF, PGF2α and insulin on S6 phosphorylation and the initiation of protein and DNA synthesis. Cell 30:235–242.
  • Traugh, J. 1981. Regulation of protein synthesis by phosphorylation, p. 167–208. In G. Litwack (ed.), Biochemical actions of hormones, vol. 8. Academic Press, Inc., New York.
  • Tsukamoto, A. S., A. Ponticelli, A. J. Berk, and R. B. Gaynor. 1986. Genetic mapping of a major site of phosphorylation in adenovirus type 2 E1A proteins. J. Virol. 59:14–22.
  • Warner, J. R., and C. Gorenstein. 1978. The ribosomal proteins of Saccharomyces cerevisiae. Methods Cell Biol. 20:45–59.
  • Wettenhall, R. E. H., P. Cohen, B. Caudwell, and R. Holland. 1982. Differential phosphorylation of ribosomal protein S6 in isolated rat hepatocytes after incubation with insulin and glucagon. FEBS Lett. 148:207–213.
  • Wettenhall, R. E. H., and F. J. Morgan. 1984. Phosphorylation of hepatic ribosomal protein S6 on 80 and 40S ribosomes: primary structure of S6 in the region of the major phosphorylation sites for cAMP-dependent protein kinases. J. Biol. Chem. 259:2084–2091.
  • Zinder, N., and J. D. Boeke. 1982. The filamentous phage (Ff) as vectors for recombinant DNA—a review. Gene 19:1–10.

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