The Amino-Terminal Region of the Adenovirus Serotype 5 E1a Protein Performs Two Separate Functions when Expressed in Primary Baby Rat Kidney Cells

LITERATURE CITED

• Bellett, A. J. D., P. Li, E. T. David, E. J. Mackey, A. W. Braithwaite, and J. R. Cutt. 1985. Control functions of adenovirus transformation region E1A products in rat and human cells. Mol. Cell. Biol. 5:1933–1939.
   PubMedGoogle Scholar
• Braithwaite, A. W., B. F. Cheetham, P. Li, C. R. Parish, L. K. Waldron-Stevens, and A. J. D. Bellett. 1983. Adenovirus-in-duced alterations of the cell growth cycle: a requirement for the expression of E1A but not of E1B. J. Virol. 45:192–199.
   PubMedGoogle Scholar
• Braithwaite, A. W., J. D. Murray, and A. J. D. Bellet. 1981. Alterations to controls of cellular DNA synthesis by adenovirus infection. J. Virol. 39:331–340.
   PubMedGoogle Scholar
• Feldman, L. T., and J. R. Nevins. 1982. Localization of the adenovirus El A protein—a positive acting transcriptional factor in infected cells. Mol. Cell. Biol. 3:829–838.
   Google Scholar
• Feramisco, J. R., M. Gross, T. Kamata, M. Rosenberg, and R. W. Sweet. 1984. Microinjection of the oncogene form of the human H-ras T24 protein results in rapid proliferation of quiescent cells. Cell 38:109–118.
   PubMed Web of Science ®Google Scholar
• Fisher, P. B., L. E. Babiss, B. Weinstein, and H. S. Ginsberg. 1982. Analysis of type 5 adenovirus transformation with a cloned rat embryo cell line (CREF). Proc. Natl. Acad. Sci. USA 79:3527–3531.
   PubMedGoogle Scholar
• Graham, F. L. 1984. Transformation by and oncogenicity of human adenoviruses, p. 339–398. In H. Ginsberg (ed.), The adenoviruses. Plenum Publishing Corp., New York.
   Google Scholar
• Graham, F. L., J. S. Smiley, W. C. Russel, and R. Nairn. 1977. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36:59–72.
   PubMed Web of Science ®Google Scholar
• Graham, F. L., and A. J. van der Eb. 1973. Transformation of rat cells by DNA of human adenovirus 5. Virology 54:536–539.
   PubMed Web of Science ®Google Scholar
• Greenberg, M. E., and G. M. Edelman. 1983. Comparison of the 34,000-Da pp60src substrate and a 38,000-Da phosphoprotein identified by monoclonal antibodies. J. Biol. Chem. 258:8497–8502.
   PubMedGoogle Scholar
• Haley, K. P., G. Overhauser, L. E. Babiss, H. S. Ginsberg, and N. C. Jones. 1984. Transformation properties of type 5 adenovirus mutants that differentially express the E1A gene products. Proc. Natl. Acad. Sci. USA 81:5734–5738.
   PubMedGoogle Scholar
• Harlow, E., B. R. Franza, and C. Schley. 1985. Monoclonal antibodies specific for adenovirus early region 1A: extensive heterogeneity in early region 1A products. J. Virol. 55:533–546.
   PubMed Web of Science ®Google Scholar
• Houweling, A., P. J. van den Elsen, and A. J. van der Eb. 1980. Partial transformation of primary rat cells by the leftmost 4.5% fragment of adenovirus 5 DNA. Virology 105:537–550.
   PubMed Web of Science ®Google Scholar
• Hurwitz, D., and G. Chinnadurai. 1985. Evidence that a second tumor antigen coded by adenovirus early gene region Ela is required for efficient cell transformation. Proc. Natl. Acad. Sci. USA 82:1663–1667.
   PubMedGoogle Scholar
• Kelekar, A., and M. D. Cole. 1987. Immortalization by c-myc H-ras and Ela oncogenes induces differential cellular gene expression and growth factor responses. Mol. Cell. Biol. 7:3899–3907.
   PubMed Web of Science ®Google Scholar
• Kimelman, D., J. S. Miller, D. Porter, and B. E. Roberts. 1985. Ela regions of the human adenoviruses and of the highly oncogenic simian adenovirus 7 are closely related. J. Virol. 53:399–409.
   PubMedGoogle Scholar
• Kingston, R. E., R. J. Kaufman, and P. A. Sharp. 1984. Regulation of transcription of the adenovirus EII promoter by Ela gene products: absence of sequence specificity. Mol. Cell. Biol. 4:1970–1977.
   PubMedGoogle Scholar
• Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 270:680–685.
   Google Scholar
• Land, H., L. F. Parada, and R. A. Weinberg. 1983. Tumorigenic conversion of primary embryo fibroblasts requires at least two oncogenes. Nature (London) 304:596–602.
   PubMed Web of Science ®Google Scholar
• Lillie, J., P. Loewenstein, M. R. Green, and M. Green. 1987. Functional domains of adenovirus type 5 Ela proteins. Cell 50:1091–1100.
   PubMedGoogle Scholar
• Lillie, J. W., M. Green, and M. R. Green. 1986. An adenovirus Ela protein region required for transformation and transcriptional repression. Cell 46:1043–1051.
   PubMed Web of Science ®Google Scholar
• Meager, A., R. Nairn, and A. C. Hughes. 1975. Analysis of transformed cell variants of BHK-21-C-13 isolated as survivors of adenovirus type 5 infections. Virology 68:41–59.
   PubMedGoogle Scholar
• Montell, C., G. Courtois, C. Eng, and A. Berk. 1984. Complete transformation by adenovirus 2 requires both Ela proteins. Cell 36:951–961.
   PubMedGoogle Scholar
• Montell, C., E. F. Fisher, M. H. Caruthers, and A. J. Berk. 1982. Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site. Nature (London) 295:380–384.
   PubMedGoogle Scholar
• Moran, E., T. Grodzicker, R. J. Roberts, M. B. Mathews, and B. Zerler. 1986. Lytic and transforming functions of individual products of the adenovirus E1A gene. J. Virol. 57:765–775.
   PubMedGoogle Scholar
• Moran, E., and B. Zerler. 1988. Interactions between cell growth-regulating domains in the products of the adenovirus Ela oncogene. Mol. Cell. Biol. 8:1756–1764.
   PubMedGoogle Scholar
• Murray, J. D., A. W. Braithwaite, I. W. Taylor, and A. J. D. Bellett. 1982. Adenovirus-induced alterations of the cell growth cycle: effects of mutations in early regions E2A and E2B. J. Virol. 44:1072–1075.
   PubMedGoogle Scholar
• Oda, K., M. Masuda-Muratta, K. Shiroki, and H. Handa. 1986. Mitogenic activity of the adenovirus type 12 Ela gene induced by hormones in rat cells. J. Virol. 58:125–133.
   PubMedGoogle Scholar
• Phelps, W. C., C. L. Yee, K. Munger, and P. M. Howley. 1988. The human papillomavirus type 16 E7 gene encodes transacti-vation and transformation functions similar to those of adenovirus Ela. Cell 53:539–547.
   PubMed Web of Science ®Google Scholar
• Quinlan, M. P., and T. Grodzicker. 1987. Adenovirus Ela 12S protein induces DNA synthesis and proliferation in primary epithelial cells in both the presence and absence of serum. J. Virol. 61:673–682.
   PubMedGoogle Scholar
• Ricciardi, R. P., R. L. Jones, C. L. Cepko, P. A. Sharp, and B. E. Roberts. 1981. Expression of early adenovirus genes requires a viral encoded acidic polypeptide. Proc. Natl. Acad. Sci. USA 78:6121–6125.
   PubMed Web of Science ®Google Scholar
• Roberts, B. E., J. Miller, D. Kimelman, C. Cepko, I. Lemischka, and R. C. Mulligan. 1985. Individual adenovirus type 5 early region la gene products elicit distinct alterations of cellular morphology and gene expression. J. Virol. 56:404–413.
   PubMedGoogle Scholar
• Rowe, D. T., F. L. Graham, and P. E. Branton. 1983. Intracellular localization of adenovirus type 5 tumor antigens in productively infected cells. Virology 129:456–468.
   PubMedGoogle Scholar
• Ruley, H. E. 1983. Adenovirus early region 1A enables viral and cellular transforming genes to transform primary cells in culture. Nature (London) 304:602–606.
   PubMed Web of Science ®Google Scholar
• Schmitt, R., M. Fahnestock, and J. Lewis. 1987. Differential nuclear localization of the major adenovirus type 2 Ela proteins. J. Virol. 61:247–255.
   PubMedGoogle Scholar
• Schneider, J. F., F. Fisher, C. R. Goding, and N. C. Jones. 1987. Mutational analysis of the adenovirus Ela gene: the role of transcriptional regulation in transformation. EMBO J. 6:2053–2060.
   PubMedGoogle Scholar
• Smith, D. H., D. Kegler, and E. B. Ziff. 1985. Vector expression of adenovirus type 5 Ela proteins: evidence for Ela autoregu-lation. Mol. Cell. Biol. 5:2684–2696.
   PubMedGoogle Scholar
• Stabel, S., P. Argos, and L. Phitipson. 1985. The release of growth arrest by microinjection of adenovirus E1A DNA. EMBO J. 4:2329–2336.
   PubMed Web of Science ®Google Scholar
• Stephens, C., and E. Harlow. 1987. Differential splicing yields novel adenovirus 5 Ela mRNAs that encode 30kd and 35kd proteins. EMBO J. 6:2027–2035.
   PubMedGoogle Scholar
• Stow, N. D. 1981. Cloning of a DNA fragment from the left-hand terminus of the adenovirus type 2 genome and its use in site-directed mutagenesis. J. Virol. 27:171–180.
   Google Scholar
• Svenson, C., and G. Akusjarvi. 1984. Adenovirus 2 early region 1A stimulates expression of both viral and cellular genes. EMBO J. 3:789–794.
   PubMed Web of Science ®Google Scholar
• Ulfendahl, P., S. Linder, J.-P. Kreive, D. Nordsvist, C. Svensson, H. Hultberg, and G. Akusjarvi. 1987. A novel adenovirus-2 Ela mRNA encoding a protein with transcription activation properties. EMBO J. 6:2037–2044.
   PubMedGoogle Scholar
• Velcich, A., and E. Ziff. 1985. Adenovirus Ela proteins repress transcription from the SV40 early promoter. Cell 40:705–716.
   PubMed Web of Science ®Google Scholar
• Winberg, G., and T. Shenk. 1984. Dissection of overlapping functions within the adenovirus type 5 Ela gene. EMBO J. 3:1907–1912.
   PubMedGoogle Scholar
• Yee, S.-P., D. T. Rowe, M. L. Tremblay, M. McDermott, and P. E. Branton. 1983. Identification of human adenovirus early region 1 products by using antisera against synthetic peptides corresponding to the predicted carboxy termini. J. Virol. 46:1003–1013.
   PubMedGoogle Scholar
• Zerler, B., B. Moran, K. Maruyama, J. Moomaw, T. Grodzicker, and H. E. Ruley. 1986. E1A amino-terminal sequences facilitate in vitro establishment and enable Ha-ras oncogenes to transform primary cells. Mol. Cell. Biol. 6:887–899.
   PubMedGoogle Scholar
• Zerler, B., R. J. Roberts, M. B. Mathews, and E. Moran. 1987. Different functional domains of the adenovirus Ela gene are involved in the regulation of host cell cycle products. Mol. Cell. Biol. 7:821–829.
   PubMed Web of Science ®Google Scholar