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Molecular and Cellular Biology Volume 9, 1989 - Issue 7
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Cell and Organelle Structure and Assembly

Recombinant Human Epidermal Growth Factor Precursor Is a Glycosylated Membrane Protein with Biological Activity

Barbara Mroczkowski1 Department of Biochemistry, School of Medicine, Vanderbilt University, Nashville, Tennessee37232
,
Martha Reich1 Department of Biochemistry, School of Medicine, Vanderbilt University, Nashville, Tennessee37232
,
Katherine Chen1 Department of Biochemistry, School of Medicine, Vanderbilt University, Nashville, Tennessee37232
,
Graeme I. Bell2 Howard Hughes Medical Institute and Departments of Biochemistry, Molecular Biology, and Medicine, The University of Chicago, Chicago, Illinois60637
&
Stanley Cohen1 Department of Biochemistry, School of Medicine, Vanderbilt University, Nashville, Tennessee37232
Pages 2771-2778 | Received 24 Oct 1988, Accepted 17 Mar 1989, Published online: 31 Mar 2023

LITERATURE CITED

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  • Dolittle, R. F., D. F. Feng, and M. S. Johnson. 1984. Computerbased characterization of epidermal growth factor precursor. Nature (London) 307:558–560.
  • Gray, A., T. J. Dull, and A. Ullrich. 1983. Nucleotide sequence of epidermal growth factor cDNA predicts a 128,000-molecular weight protein precursor. Nature (London) 303:722–725.
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  • Laemmli, U. K.. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685.
  • Mroczkowski, B., M. Reich, J. Whittaker, G. I. Bell, and S. Cohen. 1988. Expression of human epidermal growth factor precursor cDNA in transfected mouse NIH 3T3 cells. Proc. Natl. Acad. Sci. USA 85:126–130.
  • Rall, L. B., J. Scott, G. I. Bell, R. J. Crawford, J. D. Penshow, H. D. Niall, and J. P. Coghlan. 1985. Mouse prepro-epidermal growth factor synthesis by the kidney and other tissues. Nature (London) 313:228–231.
  • Russell, D. W., W. J. Schneider, T. Yamamoto, K. L. Luskey, M. S. Brown, and J. L. Goldstein. 1984. Domain map of the LDL receptor: sequence homology with the epidermal growth factor precursor. Cell 37:577–585.
  • Scott, J., M. Urdea, M. Quiroga, R. Sanchez-Pescador, N. Fong, M. Selby, W. J. Rutter, and G. I. Bell. 1983. Structure of a mouse submaxillary messenger RNA encoding epidermal growth factor and seven related proteins. Science 221:236–240.
  • Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350–4354.
  • Weissman, B. E., and S. A. Aaronson. 1983. BALB and Kirsten- murine sarcoma viruses alter growth and differentiation of EGF-dependent BALB/c mouse epidermal keratinocyte lines. Cell 32:599–606.

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