The roles of the pfcrt 76T and pfmdr1 86Y mutations, immunity and the initial level of parasitaemia, in predicting the outcome of chloroquine treatment in two areas with different transmission intensities

REFERENCES

• Adagu, I. S. & Warhurst, D. C. (2001). Plasmodium falciparum: linkage disequilibrium between loci in chromosomes 7 and 5 and chloroquine selective pressure in northern Nigeria. Parasitology, 123, 219–224.
   PubMed Web of Science ®Google Scholar
• Alifrangis, M., Enosse, S. M., Khalil, I. F., Tarimo, D. S., Lemnge, M. M., Thompson, R., Bygbjerg, I. C. & Ronn, A. M. (2003). Prediction of P. falciparum resistance to sulfadoxine/pyrimethamine in vivo by mutations in the dhfr and dhps genes — a comparative study between sites of differing endemicity. American Journal of Tropical Medicine and Hygiene, 69, 601–606.
   PubMed Web of Science ®Google Scholar
• Babiker, H. A., Pringle, S. J., Abdel-Muhsin, A., Mackinnon, M., Hunt, P. & Walliker, D. (2001). High-level chloroquine resistance in Sudanese isolates of Plasmodium falciparum is associated with mutations in the chloroquine resistance trans-porter gene pfcrt and the multidrug resistance gene pfmdrl. Journal of Infectious Diseases, 183, 1535–1538.
   PubMed Web of Science ®Google Scholar
• Basco, L. K. & Ringwald, P. (2001). Analysis of the key pfcrt point mutation and in vitro and in vivo response to chloroquine in Yaounde, Cameroon. Journal of Infectious Diseases, 183, 1828–1831.
   PubMed Web of Science ®Google Scholar
• Basco, L. K., le Bras, J., Rhoades, Z. & Wilson, C. M. (1995). Analysis of pfmdrl and drug susceptibility in fresh isolates of Plasmodium falciparum from subSaharan Africa. Molecular and Biochemical Parasitology, 74, 157–166.
   PubMed Web of Science ®Google Scholar
• Chen, N., Russell, B., Staley, J., Kotecka, B., Nasveld, P. & Cheng, Q. (2001). Sequence polymorphisms in pfcrt are strongly associated with chloroquine resis-tance in Plasmodium falciparum. Journal of Infectious Diseases, 183, 1543–1545.
   PubMed Web of Science ®Google Scholar
• Djimdé, A., Doumbo, O. K., Cortese, J. F., Kayentao, K., Doumbo, S., Diourte, Y., Dicko, A., Su, X. Z., Nomura, T., Fidock, D. A., Wellems, T. E., Plowe, C. V. & Coulibaly, D. (2001a). A molecular marker for chloroquine-resistant falciparum malaria. New England Journal of Medicine, 344, 257–263.
   PubMed Web of Science ®Google Scholar
• Djimdé, A., Doumbo, O. K., Steketee, R. W. & Plowe, C. V. (2001 b). Application of a molecular marker for surveillance of chloroquine-resistant falciparum malaria. Lancet, 358, 890–891.
   PubMed Web of Science ®Google Scholar
• Djimdé, A., Doumbo, O. K., Traore, O., Guindo, A. B., Kayentao, K., Diourte, Y., Niare-Doumbo, S., Coulibaly, D., Kone, A. K., Cissoko, Y., Tekete, M., Fofana, B., Dicko, A., Diallo, D. A., Wellems, T. E., Kwiatkowski, D. & Plowe, C. V. (2003). Clearance of drug-resistant parasites as a model for protective immunity in Plasmodium falciparum malaria. American Journal of Tropical Medicine and Hygiene, 69, 558–563.
   PubMed Web of Science ®Google Scholar
• Dorsey, G., Kamya, M. R., Singh, A. & Rosenthal, P. J. (2001). Polymorphisms in the Plasmodium falciparum pfcrt and pfmdr-1 genes and clinical response to chloroquine in Kampala, Uganda. Journal of Infectious Diseases, 183, 1417–1420.
   PubMed Web of Science ®Google Scholar
• Fidock, D. A., Nomura, T., Talley, A. K., Cooper, R. A., Dzekunov, S. M., Ferdig, M. T., Ursos, L. M., Sidhu, A. B., Naude, B., Deitsch, K. W., Su, X. Z., Wootton, J. C., Roepe, P. D. & Wellems, T. E. (2000). Mutations in the P. falciparum digestive vacuole transmembrane protein PfCRT and evidence for their role in chloroquine resistance. Molecular Cell, 6, 861–871.
   PubMed Web of Science ®Google Scholar
• Foote, S. J., Kyle, D. E., Martin, R. K., Oduola, A. M., Forsyth, K., Kemp, D. J. & Cowman, A. F. (1990). Several alleles of the multidrug-resistance gene are closely linked to chloroquine resistance in Plasmodium falciparum. Nature, 345, 255–258.
   PubMed Web of Science ®Google Scholar
• Haruki, K., Bray, P. G., Ward, S. A., Hommel, M. & Ritchie, G. Y. (1994). Chloroquine resistance of Plasmodium falciparum: further evidence for a lack of association with mutations of the pfindr1 gene. Transactions of the Royal Society of Tropical Medicine and Hygiene, 88, 694.
   PubMed Web of Science ®Google Scholar
• Khalil, I. F., Alifrangis, M., Ronn, A. M., Gabar, H. A., Jelinek, T., Satti, G. M. & Bygbjerg, I. C. (2002). Pyrimethamine/sulfadoxine combination in the treat-ment of uncomplicated falciparum malaria: relation between dihydropteroate synthase/dihydrofolate reductase genotypes, sulfadoxine plasma levels, and treatment outcome. American Journal of Tropical Medicine and Hygiene, 67, 225–229.
   PubMed Web of Science ®Google Scholar
• Laxminarayan, R. (2004). Act now or later? Economics of malaria resistance. American Journal of Tropical Medicine and Hygiene, 71 (Suppl. 2),187-195.
   Google Scholar
• Mayor, A. G., Gomez-Olive, X., Aponte, J. J., Casimiro, S., Mabunda, S., Dgedge, M., Barreto, A. & Alonso, P. L. (2001). Prevalence of the K76T mutation in the putative Plasmodium falciparum chloroquine resistance transporter (pfcrt) gene and its relation to chloroquine resistance in Mozambique. Journal of Infectious Diseases, 183, 1413–1416.
   PubMed Web of Science ®Google Scholar
• Mayxay, M., Chotivanich, K., Pukrittayakamee, S., Newton, P., Looareesuwan, S. & White, N. J. (2001). Contribution of humoral immunity to the therapeutic response in falciparum malaria. American Journal of Tropical Medicine and Hygiene, 65, 918–923.
   PubMed Web of Science ®Google Scholar
• Mita, T., Kaneko, A., Lum, J. K., Bwijo, B., Takechi, M., Zungu, I. L., Tsukahara, T., Tanabe, K., Kobayakawa, T. & Bjorkman, A. (2003). Recovery of chloroquine sensitivity and low prevalence of the Plasmodium falciparum chloroquine resistance trans-porter gene mutation K76T following the discon-tinuance of chloroquine use in Malawi. American Journal of Tropical Medicine and Hygiene, 68, 413–415.
   PubMed Web of Science ®Google Scholar
• Ochong, E. O., van den Broek, I. V., Keus, K. & Nzila, A. (2003). Association between chloroquine and arnodia-quine resistance and allelic variation in the Plasmodium falciparum multiple drug resistance 1 gene and the chloroquine resistance transporter gene in isolates from the upper Nile in southern Sudan. American Journal of Tropical Medicine and Hygiene, 69, 184–187.
   PubMed Web of Science ®Google Scholar
• Omar, S. A., Adagu, I. S. & Warhurst, D. C. (2001). Can pretreatment screening for dhps and dhfr point mutations in Plasmodium falciparum infections be used to predict sulfadoxine-pyrimethamine treat-ment failure? Transactions of the Royal Society of Tropical Medicine and Hygiene, 95, 315–319.
   PubMed Web of Science ®Google Scholar
• Pillai, D. R., Labbe, A. C., Vanisaveth, V., Hongvangthong, B., Pomphida, S., Inkathone, S., Zhong, K. & Kain, K. C. (2001). Plasmodium falciparum malaria in Laos: chloroquine treatment outcome and predictive value of molecular markers. Journal of Infectious Diseases, 183, 789–795.
   PubMed Web of Science ®Google Scholar
• Plowe, C. V. (2003). Monitoring antimalarial drug resistance: making the most of the tools at hand. Journal of Experimental Biology, 206, 3745-3752.
   Google Scholar
• Ridley, R G. (1998). Malaria: dissecting chloroquine resistance. Current Biology, 8, R346–R349.
   PubMed Web of Science ®Google Scholar
• Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Press.
   Google Scholar
• Sidhu, A. B. R., Pinard, D. V. & Fidock, D. A. (2002). Chloroquine resistance in Plasmodium falciparum malaria parasites conferred by pfcrt mutations. Science, 298, 210–213.
   PubMed Web of Science ®Google Scholar
• Talisuna, A. O., Kyosiimire-Lugemwa, J., Langi, P., Mutabingwa, T. K., Watkins, W., van Marck, E., Egwang, T. & d'Alessandro, U. (2002). Role of the pfcrt codon 76 mutation as a molecular marker for population-based surveillance of chloroquine (CQ)-resistant Plasmodium falciparum malaria in Ugandan sentinel sites with high CQ resistance. Transactions of the Royal Society of Tropical Medicine and Hygiene, 96, 551–556.
   Google Scholar
• Thomas, S. M., Ndir, O., Dieng, T., Mboup, S., Wypij, D., Maguire, J. H. & Wirth, D. F. (2002). In vitro chloroquine susceptibility and PCR analysis of pfcrt and pfmdr 1 polyrnorphisms in Plasmodium falciparum isolates from Senegal. American Journal of Tropical Medicine and Hygiene, 66, 474–480.
   PubMed Web of Science ®Google Scholar
• Von Seidlein, L., Duraisingh, M. T., Drakeley, C. J., Bailey, R., Greenwood, B. M. & Pinder, M. (1997). Polymorphism of the pfmdr 1 gene and chloroquine resistance in Plasmodium falciparum in The Gambia. Transactions of the Royal Society of Tropical Medicine and Hygiene, 91, 450–453.
   PubMed Web of Science ®Google Scholar
• Wellems, T. E. & Plowe, C. V. (2001). Chloroquine-resistant malaria. Journal of Infectious Diseases, 184, 770–776.
   PubMed Web of Science ®Google Scholar
• World Health Organization (1973). Chemotherapy of Malaria and Resistance to Antimalarials. Technical Report Series No. 529. Geneva: WHO.
   Google Scholar
• World Health Organization (1996). Assessment of Therapeutic Efficacy of Antimalarial Drugs for Uncomplicated Falciparum Malaria in Areas with Intense Transmission. Document WHO/MAL/ 96. 1077. Geneva: WHO.
   Google Scholar
• World Health Organization (2003a). The African Malaria Report. Document WHO/CDS/MAL/ 2003. 1093. Geneva: WHO.
   Google Scholar
• World Health Organization (2003b). Assessment and Monitoring of Antimalarial Drug Efficacy for the Treatment of Uncomplicated Falciparum Malaria. Document WHO/HTM/RBM/2003.50. Geneva: WHO.
   Google Scholar
• Zhang, H., Howard, E. M. & Roepe, P. D. (2002). Analysis of the antimalarial drug resistance protein Pfcrt expressed in yeast. Journal of Biological Chemistry, 277, 49,767-49,775.
   Google Scholar