A Complete Protocol for In Situ Hybridization of Messenger RNAs in Brain and Other Tissues With Radio-labeled Single-Stranded RNA Probes

References

• Maniatis T, Fritsch EF, Sanbrook J: Molecular Cloning. A Laborawry Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1982.
   Google Scholar
• Gall JG, Pardue ML: Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc Natl Acad Sci USA 63:378–383, 1969.
   PubMed Web of Science ®Google Scholar
• Cox KH, DeLeon DV, Angerer LM, Angerer RC: Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev Bio/ 101:485–502, 1984.
   PubMed Web of Science ®Google Scholar
• Melton DA, Krieg PA, Rebagliati MR et al: Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nuc Acids Res 12:7035–7056, 1984.
   PubMed Web of Science ®Google Scholar
• Davis LG, Dibner MD, Battery J: Methods in Molecular Biology. Elsevier, New York, 1986.
   Google Scholar
• Swanson LW, Sawchenko PE, Lind RW: Regulation of multiple peptides in CRF parvocellular neurosecretory neurons: Implications for the stress response. Frog Brain Res 68:169–190, 1986.
   PubMed Web of Science ®Google Scholar
• Sawchenko PE, Swanson LW, Vale WW: Corticotropin-releasing factor: Co-expression within distinct subsets of oxytocin-, vaso-pressin-, and neurotensin-immunoreactive neurons in the hypothalamus of the male rat. J Neurosci 4:1118–1129, 1984.
   PubMed Web of Science ®Google Scholar
• Swanson LW, Simmons DM, Arriza J et al: Novel developmental specificity in the nervous system of transgenic animals expressing growth hormone fusion genes. Nature 317:6035–6038, 1985.
   Google Scholar
• Goldman D, Simmons D, Swanson LW et al: Mapping of brain areas expressing RNA homologous to two different acetylcholine receptor a-subunit cDNAs. Proc Nail Acad Sci USA 83:4076–4080, 1986.
   PubMed Web of Science ®Google Scholar
• Gerfen CR, Sawchenko PE: An anterograde neuroanatomical tracing method that shows the detailed morphology of neurons, their axons and terminals: Immunohistochemical localization of an axonally transported plant lectin "Phaseolus vulgaris"leucoag-glutinin (PHA-L) Brain Res 290:219–238, 1984.
   PubMed Web of Science ®Google Scholar
• Singer RH, Lawrence JB, Villnave C: Optimization of in situ hybridization using isotopic and non-isotopic detection methods. BioTech 4:230–250, 1986.
   Web of Science ®Google Scholar
• Wahl GM, Meinkoth JL, Kimmel AR: Northern and southern blots. Meth Enzymo 1152:572–581, 1987.
   Google Scholar
• Rogers AW: Techniques of Autoradiography. 3rd edition. Elsevier, New York, 1979.
   Google Scholar
• Wada E, Wada K, Boulter J et al: The distribution of alpha 2, alpha 3, alpha 4, and beta z neuronal nicotinic receptor subunit mRNAs in the central nervous system. A hybridization histochemical study in the rat. J Comp Neurol 284:314–335, 1989.
   PubMed Web of Science ®Google Scholar
• Hendrickson A, Moe L, Noble B: Staining for autoradiography of the central nervous system. Stain Technol 47:283–290, 1972.
   PubMed Web of Science ®Google Scholar
• Swanson LW, Simmons DM: Differential steroid hormone and neural influences on peptide mRNA levels in CRH cells of the paraventricular nucleus: A hybridization histochemical study in the rat. J Comp Nurol 285:413–435, 1989.
   PubMed Web of Science ®Google Scholar
• Young WS III, Kuhar MJ: Quantitative in situ hybridization and determination of mRNA content. In: Uhl GR (ed): In Situ Hybridization in the Brain. Plenum Press, New York, pp 243–248, 1986.
   Google Scholar
• Watts AG, Swanson LW: The combination of in situ hybridization with immunohistochemistry and retrograde tract-tracing. In Methods in Neurosciences, Vol 1: Genetic Probes PM Conn (ed), in press, 1989.
   Google Scholar
• Haase AT, Stowring L, Geballe A et al: The use of 35S labeled probes to detect virus genes by in situ hybridization. NEN Product News 13(3):2–3, 1984.
   Google Scholar
• Meinkoth J, Wahl G: Hybridization of nucleic acids immobilized on solid supports. Analy Biochem 138:267–284, 1984.
   PubMed Web of Science ®Google Scholar
• De Olmos JS, Hardy H, Heimer L: The afferent connections of the main and the accessory olfactory bulb formations in the rat: An experimental HRP study. J Comp Neuro/ 181:213–244, 1978.
   PubMed Web of Science ®Google Scholar