A Double-Label Immunohistochemical Method for Assessment of Epithelial Cell Replication in Paraffin Sections of Toxicant-Damaged Lungs

References

• Grisham JW, Kaufmann WK, Kaufman DG: The cell cycle and chemical carcinouenesis. Surv Synth Pathol Res 1:49–66, 1983
   Google Scholar
• Sorokin SP: The respiratory system. In Histology, Weiss L. Greep RD (eds), McGraw Hill Book Company, New York, 1977, pp 765–782
   Google Scholar
• Adamson IYR: Cellular kinetics of the lung. In Toxicology of Inhaled Materials, Witschi HP and Brain JD (eds), Springer-Verlag, New York, 1985, pp 289–317
   Google Scholar
• Taya A., Morgan A. Baker ST et al: Changes in the rat lung after exposure to radon and its progeny: effects on incorporation of bromodeoxyuridine in epithelial cells and on the incidence of nuclear aberrations in alveolar macrophages. Radiat Res 139:170–177, 1994
   PubMed Web of Science ®Google Scholar
• Donaldson K. Brown DM, Miller BG, Brody A: Bromo-deoxyuridine (BrdU) uptake in the lungs of rats inhaling amsite or vitreous fibers at equal airborne fiber concentrations. Exp Toxicol Pathol 47:207–211, 1995
   Web of Science ®Google Scholar
• Lee C, Rajini P. Pinkerton KE, WitschiCorrelation between cumulative labeling indices measured in the terminal bronchioles and in the centriacinar region in the lungs of rats exposed to oxidant air pollutants. Toxicol Lett 80:25–28, 1995
   PubMed Web of Science ®Google Scholar
• MaiBe KA, Quinlan TR, Moulton G et al: Comparative proliferative and histopathologic changes in rat lungs after inhalation of chrysotile or crocidolite asbestos. Toxicol Appl Phutmacol 137:67–74, 1996
   PubMed Web of Science ®Google Scholar
• S. Silvestrini R. Costa A, Veneroni S et al: Comparative analysis of different approaches to investigate cell kinetics. Cell Tissue Kinet 21:123–131, 1988
   PubMedGoogle Scholar
• Moran R, Darzynkiewicz Z. Staiano-Coico L. Melamed MR: Detection of 5-bromodeoxyuridine (BrdU) incorporation by monoclonal antibodies: role of the denaturation step. J Histochem Cvtochem 33:821–827, 1985
   PubMed Web of Science ®Google Scholar
• Veronese S., Gambacorta M. Falini BTI: In situ demonstration of tissue proliferative activity using antibromo-deoxyuridine monoclonal antibody. .J Clin Pathol 42:820–826, 1989
   PubMed Web of Science ®Google Scholar
• Tapia FJ, Varndell IM, Probed L, et al: Double immunogold staining method for the simultaneous ultrastructural localization of regulatory peptides. J Histochem Cytochem 31:977–981, 1983
   PubMed Web of Science ®Google Scholar
• Avivi C. Rosen O, Goldstein RS: New chromogenes for alkaline phosphatase histochemistry: salmon and magenta phosphate are useful for single and doublelabel immunohisto-chemistry. J Histochem Cytochem 42:551–554, 1994
   PubMed Web of Science ®Google Scholar
• Green JA, Manson MM: Double label immunohistochemistry on tissue sections using alkaline phosphatase and peroxidase conjugates. Methods Mol Biol 80:271–274, 1998
   PubMedGoogle Scholar
• Krenacs T, Krenacs L, Raffeld M: Multiple antigen immuno-staining procedures. Methods Mol Biol 115:223–233, 1999
   PubMedGoogle Scholar
• Schutz A., Tannapfe A. Wittekind C: Comparison of different double immunostaining protocols for paraffin embedded liver tissue. Anal Cell Pathol 18:227–233, 1999
   PubMed Web of Science ®Google Scholar
• Finch GL, Nikula KJ, Chen BT et al: Effect of chronic cigarette smoke exposure on lung clearance of tracer particles inhaled by rats. Fundam Appl Toxicol 24:76–85, 1995
   PubMedGoogle Scholar
• Harkema JR, Hotchkiss JA: In vli'o effects of endotoxin on intraepithelial mucosubstances in the rat pulmonary airways. Quantitative histochemistry. Am J Pathol 141:307–317, 1992
   PubMed Web of Science ®Google Scholar
• Harkema JR, Hotchkiss JA: In vivo effects of endotoxin on DNA synthesis in rat nasal epithelium. Microsc Res Tech 26(5):457–465, 1993
   PubMed Web of Science ®Google Scholar
• Goldsworthy TL, Morgan KT, Popp JA, Butterworth BE: Guidelines for measuring chemically induced cell proliferation in specific rodent target organs. In Chemically Induced Cell Proliferation: Implications pr Risk Assessment, Butter-worth BE, Slaga Ti. Farland W., McClain M (eds), Wiley-Liss, Inc., NY, 1991, pp 253–284
   Google Scholar
• Kasper M, Rudolf T. Verhosfstad AAJ et al: Heterogeneity in the immunolocalization of cytokeratin-specific monoclonal antibodies in the rat lung: evaluation of three different alveolar epithelial cell types. Histochemisti:v 100:65–71, 1993
   PubMedGoogle Scholar
• Franke WW, Winter S. von Overbeck J et al: Identification of the conserved, conformation-dependent cytokeratin epitope recognized by monoclonal antibody (10–5). Virchows Arch A 411:137–147, 1987
   Google Scholar
• Foley JF, Dietrich DR, Swenberg JA, Maronpot RR: Detection and evaluation of proliferating cell nuclear antigen (PCNA) in rat tissue by an improved immunohistochemical procedure. J Histotechnol 14:237–239, 1991
   Web of Science ®Google Scholar