- 1) Rocklin, R. D., Ramsey, R. S., and Ramsey, J. M., A microfabricated fluidic device for performing two-dimensional liquid-phase separations. Anal. Chem., 72, 5244–5249 (2000).
- 2) Link, A. J., Eng, J., Schieltz, D. M., Carmack, E., Mize, G. J., Morris, D. R., Garvik, B. M., and Yates, J. R., Direct analysis of protein complexes using mass spectrometry. Nat. Biotech., 17, 676–682 (1999).
- 3) Bushey, M. M., and Jorgenson, J. W., Automated instrumentation for comprehensive two-dimensional high-performance liquid chromatography of proteins. Anal. Chem., 62, 161–167 (1990).
- 4) Holland, L. A., and Jorgenson, J. W., Separation of nanoliter samples of biological amines by a comprehensive two-dimensional microcolumn liquid chromatography system. Anal. Chem., 67, 3275–3283 (1995).
- 5) Opiteck, G. J., Lewis, K. C., Jorgenson, J. W., and Anderegg, R. J., Comprehensive on-line LC/LC/MS of Proteins. Anal. Chem., 69, 1518–1524 (1997).
- 6) Opiteck, G. J., Jorgenson, J. W., and Anderegg, R. J., Two-dimensional SEC/RPLC coupled to mass spectrometry for the analysis of peptides. Anal. Chem., 69, 2283–2291 (1997).
- 7) Kohne, A. P., and Welsch, T., Coupling of a microbore column with a column packed with non-porous particles for fast comprehensive two-dimensional high-performance liquid chromatography. J. Chromatogr. A, 845, 463–469 (1999).
- 8) Wagner, K., Racaityte, K., Unger, K. K., Miliotis, T., Edholm, L., Bischoff, R., and Marko-Varga, G., Protein mapping by two-dimensional high performance liquid chromatography. J. Chromatogr. A, 893, 293–305 (2000).
- 9) Unger, K. K., Racaityte, K., Wagner, K., Miliotis, T., Edholm, L. E., Bischoff, R., and Marco-Varga, G., Is multidimensional high performance liquid chromatography (HPLC) an alternative in protein analysis to 2D gel electrophoresis? J. High Resol. Chromatogr., 23, 259–265 (2000).
- 10) Wagner, K., Miliotis, T., Marko-Varga, G., Bischoff, R., and Unger, K. K., An automated on-line multidimensional HPLC system for protein and peptide mapping with integrated sample preparation. Anal. Chem., 74, 809–820 (2002).
- 11) Haefliger, O. P., Universal two-dimensional HPLC technique for the chemical analysis of complex surfactant mixtures. Anal. Chem., 75, 371–378 (2003).
- 12) Davis, M. T., Beierle, J., Bures, E. T., McGinley, M. D., Mort, J., Robinson, J. H., Spahr, C. S., Yu, W., Luethy, R., and Patterson, S. D., Automated LC–LC–MS–MS platform using binary ion-exchange and gradient reversed-phase chromatography for improved proteomic analyses. J. Chromatogr. B, 752, 281–291 (2001).
- 13) Minakuchi, H., Nakanishi, K., Soga, N., Ishizuka, N., and Tanaka, N., Octadecylsilylated porous silica rods as separation media for reversed-phase liquid chromatography. Anal. Chem., 68, 3498–3501 (1996).
- 14) Minakuchi, H., Nakanishi, K., Soga, N., Ishizuka, N., and Tanaka, N., Effect of domain size on the performance of octadecylsilylated continuous porous silica columns in reversed-phase liquid chromatography. J. Chromatogr. A, 797, 121–131 (1998).
- 15) Motokawa, M., Kobayashi, H., Ishizuka, N., Minakuchi, H., Nakanishi, K., Jinnai, H., Hosoya, K., Ikegami, T., and Tanaka, N., Monolithic silica columns with various skeleton sizes and through-pore sizes for capillary liquid chromatography. J. Chromatogr. A, 961, 53–63 (2002).
- 16) Chunling, W., Ron, C., and David, S. H., Obtaining high sequence coverage in matrix-assisted laser desorption time-of-fight mass spectrometry for studies of protein modification: analysis of human serum albumin as a model. Anal. Biochem., 349, 229–241 (2006).
- 17) Kimura, H., Tanigawa, T., Morisaka, H., Ikegami, T., Hosoya, K., Ishizuka, N., Minakuchi, H., Nakanishi, K., Ueda, M., Cabrera, K., and Tanaka, N., Simple 2D-HPLC using a monolithic silica column for peptide separation. J. Sep. Sci., 27, 897 (2004).
- 18) Rejtar, T., Hu, P., Juhasz, P., Campbell, J. M., Vestal, M. L., Preisler, J., and Karger, B. L., Off-line coupling of high-resolution capillary electrophoresis to MALDI-TOF and TOF/TOF MS. J. Proteome Res., 1, 171–179 (2002).
- 19) Witt, M., Fuchser, J., and Baykut, G., Fourier transform ion cyclotron resonance mass spectrometry with nanolc/microelectrospray ionization and matrix-assisted laser desorption/ionization: analytical performance in peptide mass fingerprint analysis. J. Am. Soc. Mass Spectrom., 14, 553–561 (2003).
Full access
Enhanced Sequence Coverage in Tryptic Fragment Analysis by Two-Dimensional HPLC/MS Using a Monolithic Silica Capillary Column
Reprints and Corporate Permissions
Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?
To request a reprint or corporate permissions for this article, please click on the relevant link below:
Academic Permissions
Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?
Obtain permissions instantly via Rightslink by clicking on the button below:
If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.
Related research
People also read lists articles that other readers of this article have read.
Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.
Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.