Advanced search
Publication Cover
Bioscience, Biotechnology, and Biochemistry Volume 62, 1998 - Issue 7
Journal homepage
190
Views
7
CrossRef citations to date
0
Altmetric
Original Articles

Molecular Cloning and Expression in Saccharomyces cerevisiae of Tobacco NADPH-Cytochrome P450 Oxidoreductase cDNA

Takashi YAMADADepartment of Biological and Environmental Science, Faculty of Agriculture, Kobe University
,
Hiromasa IMAISHIDepartment of Biological and Environmental Science, Faculty of Agriculture, Kobe University
,
Atsuhiro OKALaboratory of Molecular Genetics, Institute for Chemical Research, Kyoto University
&
Hideo OHKAWADepartment of Biological and Environmental Science, Faculty of Agriculture, Kobe University
Pages 1403-1411 | Received 25 Feb 1998, Published online: 22 May 2014

  • 1) Schuler, M. A., Plant Cytochrome P450 Monooxygenases. Crit. Rev. Plant Sci., 15, 235-284 (1996).
  • 2) Iyanagi, T., Makino, R., and Anan, F. K., Studies on the microsomal mixed-function oxidase system: mechanism of action of hepatic NADPH-cytochrome P-450 reductase. Biochemistry, 20, 1722-1730 (1981).
  • 3) Imaishi, H., Yamada, T., and Ohkawa, H., Purification and immunochemical characteristics of NADPH-cytochrome P-450 oxidoreductase from tobacco cultured cells. Biochim. Biophys. Acta, 1246, 53-60 (1995).
  • 4) Oeda, K., Sakaki, T., and Ohkawa, H., Expression of rat liver cytochrome P-450MC cDNA in Saccharomyces cerevisiae. DNA, 4, 203-210 (1985).
  • 5) Urban, P., Mignotte, C., Kazmaier, M., Delorme, F., and Pompon, D., Cloning, yeast expression, and characterization of the coupling of two distantly related Arabidopsis thaliana NADPH-cytochrome P450 reductases with P450 CYP73A5. J. Biol. Chem., 272, 19176-19186 (1997).
  • 6) Shet, M. S., Sathasivan, K., Arlotto, M. A., Mehdy, M. C., and Estabrook, R. W., Purification, characterization, and cDNA cloning of an NADPH-cytochrome P450 reductase from mung bean. Proc. Natl. Acad. Sci. USA, 90, 2890-2894 (1993).
  • . 1989.
  • 8) Triglia, T., Peterson, M. G., and Kemp, D. J., A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences. Nucleic Acids Res., 16, 8186 (1988).
  • 9) Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J., Basic local alignment search tool. J. Mol. Biol., 215, 403-410 (1990).
  • 10) Ravelonandro, M. and Dunez, J., Rapid and efficient techniques for detecting transgenic tobacco plants, Biotechniques, 14, 186-190 (1993).
  • 11) Matsudaira, P., Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J. Biol. Chem., 262, 10035-10038 (1987).
  • 12) Ito, H., Fukuda, Y., Murata, K., and Kimura, A., Transformation of intact yeast cells treated with alkali cations. J. Bacteriol., 153, 163-168 (1983).
  • 13) Cavener, D. R. and Ray, S. C., Eukaryotic start and stop translation sites. Nucleic Acids Res., 19, 3185-3192 (1991).
  • 14) Yabusaki, Y., Murakami, H., and Ohkawa, H., Primary structure of Saccharomyces cerevisiae NADPH-cytochrome P450 reductase deduced from nucleotide sequence of its cloned gene. J. Biochem. (Tokyo), 103, 1004-1010 (1988).
  • 15) Porter, T. D. and Kasper, C. B., Coding nucleotide sequence of rat NADPH-cytochrome P-450 oxidoreductase cDNA and identification of flavin-binding domains. Proc. Natl. Acad. Sci. USA, 82, 973-977 (1985).
  • 16) Wang, M., Roberts, D. L., Paschke, R., Shea, T. M., Masters, B. S., and Kim, J. J., Three-dimensional structure of NADPH-cytochrome P450 reductase: prototype for FMN- and FAD-containing enzymes. Proc. Natl. Acad. Sci. USA, 94, 8411-8416 (1997).
  • 17) Meijer, A. H., Lopes Cardoso, M. I., Voskuilen, J. T., de Waal, A., Verpoorte, R., and Hoge, J. H., Isolation and characterization of a cDNA clone from Catharanthus roseus encoding NADPH:cytochrome P-450 reductase, an enzyme essential for reactions catalysed by cytochrome P-450 mono-oxygenases in plants. Plant. J., 4, 47-60 (1993).
  • 18) Laudert, D., Pfannschmidt, U., Lottspeich, F., Hollander-Czytko, H., and Weiler, E. W., Cloning, molecular and functional characterization of Arabidopsis thaliana allene oxide synthase (CYP74), the first enzyme of the octadecanoid pathway to jasmonates. Plant Mol. Biol., 31, 323-335 (1996).
  • 19) Nisimoto, Y., Localization of cytochrome c-binding domain on NADPH-cytochrome P-450 reductase. J. Biol. Chem., 261, 14232-14239 (1986).
  • 20) Black, S. D., French, J. S., Williams, C. H. Jr., and Coon, M. J., Role of a hydrophobic polypeptide in the N-terminal region of NADPH-cytochrome P-450 reductase in complex formation with P-450LM. Biochem. Biophys. Res. Commun., 91, 1528-1535 (1979).
  • 21) Shen, A. L. and Kasper, C. B., Role of acidic residues in the interaction of NADPH-cytochrome P450 oxidoreductase with cytochrome P450 and cytochrome c. J. Biol. Chem., 270, 27475-27480 (1995).
  • 22) Simmons, D. L., Lalley, P. A., and Kasper, C. B., Chromosomal assignments of genes coding for components of the mixed-function oxidase system in mice. Genetic localization of the cytochrome P-450PCN and P-450PB gene families and the NADPH-cytochrome P-450 oxidoreductase and epoxide hydratase genes. J. Biol. Chem., 260, 515-521 (1985).
  • 23) Lesot, A., Hasenfratz, M.-P., Batard, Y., Durst, F., and Benveniste, I., Two messenger RNAs are encoding for NADPH-cytochrome P450 reductases in Helianthus tuberosus tuber tissues. Plant Physiol. Biochem., 33, 751-757 (1995).
  • 24) Murakami, H., Yabusaki, Y., and Ohkawa, H., Expression of rat NADPH-cytochrome P-450 reductase cDNA in Saccharomyces cerevisiae. DNA, 5, 1-10 (1986).
  • 25) Murakami, H., Yabusaki, Y., Sakaki, T., Shibata, M., and Ohkawa, H., Expression of cloned yeast NADPH-cytochrome P450 reductase gene in Saccharomyces cerevisiae. J. Biochem. (Tokyo), 108, 859-865 (1990).

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.