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Bioscience, Biotechnology, and Biochemistry Volume 62, 1998 - Issue 10
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Original Articles

P1-Specificity of Aqualysin I (a Subtilisin-type Serine Protease) from Thermus aquaticus YT-1, using P1-Substituted Derivatives of Streptomyces Subtilisin Inhibitor

Terumichi TANAKADepartment of Biotechnology, The University of Tokyo
,
Hiroshi MATSUZAWADepartment of Biotechnology, The University of Tokyo
,
Shuichi KOJIMADepartment of Industrial Chemistry, Faculty of Engineering, The University of Tokyo
,
Izumi KUMAGAIDepartment of Industrial Chemistry, Faculty of Engineering, The University of Tokyo
,
Kin-ichiro MIURADepartment of Industrial Chemistry, Faculty of Engineering, The University of Tokyo
&
Takahisa OHTADepartment of Biotechnology, The University of Tokyo
Pages 2035-2038 | Received 20 Apr 1998, Published online: 22 May 2014

  • 1) Matsuzawa, H., Hamaoki, M., and Ohta, T., Production of thermophilic extracellular proteases (aqualysins I and II) by Thermus aquaticus YT-1, an extreme thermophile. Agric. Biol. Chem., 47, 25-28 (1983).
  • 2) Matsuzawa, H., Tokugawa, K., Hamaoki, M., Mizoguchi, M., Taguchi, H., Terada, I., Kwon, S.-T., and Ohta, T., Purification and characterization of aqualysin I (a thermophilic alkaline serine protease) produced by Thermus aquaticus YT-1. Eur. J. Biochem., 171, 441-447 (1988).
  • 3) Kwon, S.-T., Terada, I., Matsuzawa, H., and Ohta, T., Nucleotide sequence of the gene for aqualysin I (a thermophilic alkaline serine protease) of Thermus aquaticus YT-1 and characteristics of the deduced primary structure of the enzyme. Eur. J. Biochem., 173, 491-497 (1988).
  • 4) Kwon, S.-T., Matsuzawa, H., and Ohta, T., Determination of the positions of the disulfide bonds in aqualysin I (a thermophilic alkaline serine protease) of Thermus aquaticus YT-1. J. Biochem., 104, 557-559 (1988).
  • 5) Terada, I., Kwon, S.-T., Miyata, Y., Matsuzawa, H., and Ohta, T., Unique precursor structure of an extracellular protease, aqualysin I, with NH2- and COOH-terminal pro-sequences and its processing in Escherichia coli. J. Biol. Chem., 265, 6576-6581 (1990).
  • 6) Lee, Y.-C., Miyata, Y., Terada, I., Ohta, T., and Matsuzawa, H., Involvement of NH2-terminal pro-sequence in the position of active aqualysin I (a thermophilic serine protease) in Escherichia coli. Agric. Biol. Chem., 55, 3027-3032 (1991).
  • 7) Lee, Y.-C., Ohta, T., and Matsuzawa, H., A non-covalent NH2-terminal pro-region aids the production of active aqualysin I (a thermophilic protease) without the COOH-terminal pro-sequence in Escherichia coli. FEMS Microbiol. Lett., 92, 73-78 (1992).
  • 8) Tanaka, T., Matsuzawa, H., and Ohta, T., Stability of thermostable enzyme, aqualysin I; a subtilisin-type serine protease from Thermus aquaticus YT-1. Biosci. Biotechnol. Biochem., in press.
  • 9) Ikenaka, T., Odani, S., Sakai, M., Nabeshima, Y., and Murao, S., Amino acid sequence of an alkaline proteinase inhibitor (Streptomyces subtilisin inhibitor) from Streptomyces albogriseolus S-3253. J. Biochem., 76, 1191-1209 (1974).
  • 10) Obata, S., Taguchi, S., Kumagai, S., and Miura, K., Molecular cloning and nucleotide sequence determination of gene encoding Streptomyces subtilisin inhibitor (SSI). J. Biochem., 105, 367-371 (1989).
  • 11) Obata, S., Furukubo, S., Kumagai, I., Takahashi, H., and Miura, K., High-level expression in Streptomyces lividans 66 of a gene encoding Streptomyces subtilisin inhibitor from Streptomyces albogriseolus S-3253. J. Biochem., 105, 372-376 (1989).
  • 12) Miura, K., Kumagai, I., Obata, S., Kojima, S., and Taguchi, S., Partial alternation of a protein Streptomyces subtilisin inhibitor by site-directed mutagenesis. Proc. Japan Acad., 64, 147-149 (1988).
  • 13) Kojima, S., Nishiyama, Y., Kumagai, I., and Miura, K., Inhibition of subtilisin BPN’ by reaction site P1 mutants of Streptomyces subtilisin inhibitor. J. Biochem., 109, 377-382.
  • 14) Ishikawa, K., Mitsui, Y., and Iitaka, Y., Aspects of protein-protein interaction as studied through crystal structure of subtilisin complexed with its trapped substrate SSI. Proc. Symposium on Three-dimensional Structure and Drug Action., 165-175 (1987).
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  • 17) Russell, A. J. and Fersht, A. R., Rational modification of enzyme catalysis by engineering surface charge. Nature, 328, 496-500 (1987).

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