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Bioscience, Biotechnology, and Biochemistry Volume 62, 1998 - Issue 10
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Original Articles

Comparison of Phytolacain G, a Cysteine Protease from Fruit of Phytolacca americana, with Phytolacain R

Tetsuya UCHIKOBADepartment of Chemistry, Faculty of Science, Kagoshima University
,
Hiroo YONEZAWADepartment of Chemistry, Faculty of Science, Kagoshima University
,
Masayuki SHIMADADepartment of Chemistry, Faculty of Science, Kagoshima University
&
Makoto KANEDADepartment of Chemistry, Faculty of Science, Kagoshima University
Pages 2058-2061 | Received 27 May 1998, Published online: 22 May 2014

  • 1) Uchikoba, T., Yonezawa, H., Hamada, T., Matsueda, R., and Kaneda, M., Isolation of phytolacain G and its inhibition measured by affinity labeling. Biosci. Biotech. Biochem., 59, 1784-1785 (1995).
  • 2) Kaneda, M, Izumi, S., Fukuda, T., Uchikoba, T., and Tominaga, N., Isolation and characterization of a protease from Phytolacca americana. Phytochemistry, 27, 3661-3662 (1988).
  • . 1986. p. 67- 96.
  • 4) Kaneda, M, Nagatome, S., and Uchikoba, T., Comparison of phytolacain R, a cysteine protease from Phytolacca americana, with papain. Phytochemistry, 39, 997-999 (1995).
  • 5) Kunitz, M., Crystalline soybean trypsin inhibitor. J. Gen. Physiol., 30, 291-310 (1947).
  • 6) Brockbank, W. J. and Lynn, K. R., Purification and preliminary characterization of two asclepains from the latex of Asclepias syriaca L. (milkweed). Biochim. Biophys. Acta, 578, 13-22 (1979).
  • 7) Dubois, T., Jacquet, A., Schnek, A.G., and Looze, Y., The thiol proteinases from the latex of Carica papaya L. 1. Fractionation, purification and preliminary characterization. Biol. Chem. Hoppe-Seyler, 369, 733-740 (1988).
  • 8) Kaneda, M., Arima, K., Yonezawa, H., and Uchikoba, T., Purification and properties of a protease from the sarcocarp of bead tree fruit. Phytochemistry, 35, 1395-1398 (1994).
  • . 1967. p. 197- 199.
  • 10) Asboth, B. Majer, Z. and Polgar, L., Cysteine proteases: the S2P2 hydrogen bond is more important for catalysis than is the analogous S1P1 bond. FEBS Lett., 233, 339-341 (1988).
  • . 1977. p. 111- 116.
  • 12) Ritonja, A., Rowan, A. D., Buttle, D. J., Rawlings, N. D., Turk, V., and Barrett, A. J., Stem bromelain: amino acid sequence and implications for weak binding of cystatin. FEBS letters, 247, 419-424 (1989).
  • . 1972. p. D-121.
  • 14) Carne, A. and Moore, C. H., The amino acid sequence of the tryptic peptides from actinidin, a proteolytic enzyme from the fruit of Actinidia chinensis. Biochem. J., 173, 73-83 (1978).
  • . 1994. p. 461- 486.

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