116
Views
24
CrossRef citations to date
0
Altmetric
Original Articles

Isolation of the creA Gene from the Cellulolytic Fungus Humicola grisea and Analysis of CreA Binding Sites Upstream from the Cellulase Genes

, , , &
Pages 2364-2370 | Received 07 Jul 1998, Accepted 28 Aug 1998, Published online: 22 May 2014

  • 1) Dowzer, C. E. A. and Kelly, J. M. Analysis of the creA gene, a regulator of carbon catabolite repression in Aspergillus nidulans. Mol. Cell. Biol., 11, 5701-5709 (1991).
  • 2) Cubero, B. and Scazzocchio, C. Two different, adjacent and divergent zinc finger binding sites are necessary for CREA-mediated carbon catbolite repression in the proline gene cluster of Aspergillus nidulans. EMBO J., 13, 407-415 (1994).
  • 3) Kulmburg, P., Mathieu, M., Dowzer, C., Kelly, J., and Felenbok, B. Specific binding sites in the alcR and alcA promoters of the ethanol regulon for the CREA repressor mediating carbon catabolite repression in Aspergillus nidulans. Mol. Microbiol., 7, 847-857 (1993).
  • 4) Mathieu, M. and Felenbok, B. The Aspergillus nidulans CREA protein mediates glucose repression of the ethanol regulon at various levels through competition with the ALCR-specific transactivator. EMBO J., 13, 4022-4027 (1994).
  • 5) Piñaga, F., Fernández-Espinar, M. T., Vallés, S., and Ramón, D. Xylanase production in Aspergillus nidulans: Induction and carbon catabolite repression. FEMS Microbiol. Lett., 115, 319-324 (1994).
  • 6) Nehlin, J. O., and Ronne, H. Yeast MIG1 repressor is related to the mammalian early growth response and Wilms’ tumour finger protein. EMBO J., 9, 2891-2898 (1990).
  • 7) Espeso, E. A. and Peñalva, M. A. In vitro binding of the two-finger repressor CreA to several consensus and non-consensus sites at the ipnA upstream region is context dependent. FEBS Lett., 342, 43-48 (1994).
  • 8) Strauss, J., Mach, R. L., Zeilinger, S., Hartler, G., Stöffler, G., Wolschek, M., and Kubicek, C.P. Cre1, the carbon catabolite repressor protein from Trichoderma reesei. FEBS Lett., 376, 103-107 (1995).
  • 9) Takashima, S., Nakamura, A., Iikura, H., Masaki, H., and Uozumi, T. Cloning of a gene encoding a putative carbon catabolite repressor from Trichoderma reesei. Biosci. Biotech. Biochem., 60, 173-176 (1996).
  • 10) Takashima, S., Iikura, H., Nakamura, A., Masaki, H., and Uozumi, T. Analysis of Cre1 binding sites in the Trichoderma reesei cbh1 upstream region. FEMS Microbiol. Lett. 145, 361-366 (1996).
  • 11) Takashima, S., Nakamura, A., Masaki, H., and Uozumi, T. Purification and characterization of cellulases from Humicola grisea. Biosci. Biotechnol. Biochem., 60, 77-82 (1996).
  • 12) Hayashida, S. and Yoshioka, H. Production and purification of thermostable cellulases from Humicola insolens YH-8. Agric. Biol. Chem., 44, 1721-1728 (1980).
  • 13) Takashima, S., Nakamura, A., Hidaka, M., Masaki, H., and Uozumi, T. Cloning, sequencing, and expression of the cellulase genes of Humicola grisea var. thermoidea. J. Biotechnol., 50, 137-147 (1996).
  • 14) Takashima, S., Nakamura, A., Masaki, H., and Uozumi, T. Cloning, sequencing, and expression of a thermostable cellulase gene of Humicola grisea. Biosci. Biotechnol. Biochem., 61, 245-250 (1997).
  • 15) Takashima, S., Iikura, H., Nakamura, A., Hidaka, M., Masaki, H., and Uozumi, T. Isolation of the gene and characterization of the enzymatic properties of a major exoglucanase of Humicola grisea without a cellulose-binding domain. J. Biochem., 124, 717-725 (1998).
  • 16) Sambrook, J., Fritsch, E. F., and Maniatis, T., In “Molecular Cloning: a Laboratory Manual,” 2nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1989.
  • 17) Hidaka, M., Yoshida, Y., Masaki, H., and Uozumi, T. Cleavage of potato virus Y polyprotein in Escherichia coli depending on the proteolytic activity of viral protease. Biosci. Biotech. Biochem., 57, 710-714 (1993).
  • 18) Tonouchi, N., Shoun, H., Uozumi, T., and Beppu, T. Cloning and sequencing of a gene for Mucor rennin, an aspartate protease from Mucor pusillus. Nucleic Acids Res., 14, 7557-7568 (1986).
  • 19) Sanger, F., Nicklen, S., and Coulson, A. R. DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. U.S.A., 74, 5463-5467 (1977).
  • 20) Mary, R. D., Sharon, E. K., and Joan, M. K. The Aspergillus niger carbon catabolite repressor encoding gene, creA. Gene, 130, 241-245 (1993).
  • 21) Jonathan, D. L., Martha, J. G., James, F., and Ulla, M. H. Drosophila Krüppel protein is a transcriptional repressor. Nature, 346, 76-79 (1990).
  • 22) Albert, J. C. and Robert, T. Analysis of Sp1 in vivo reveals multiple transcriptional domains, including a novel glutamine-rich activation motif. Cell, 55, 887-898 (1988).

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.