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Bioscience, Biotechnology, and Biochemistry Volume 64, 2000 - Issue 6
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Original Articles

Assay of DNA Photolyase Activity in Spinach Leaves in Relation to Cell Compartmentation-Evidence for Lack of DNA Photolyase in Chloroplasts

Megumi HADADepartment of Biology, Faculty of Science, Kobe University
,
Keisuke HINODepartment of Biology, Faculty of Science, Kohnan University
,
Günther BUCHHOLZInstitute of Biology II, Freiburg University
,
Jörn GOSSInstitute of Biology II, Freiburg University
,
Eckard WELLMANNInstitute of Biology II, Freiburg University
&
Masateru SHINTeru Biolaboratory
Pages 1288-1291 | Received 22 Nov 1999, Accepted 17 Feb 2000, Published online: 22 May 2014

  • 1) Blumthaler, M., and Amback, W., Induction of increasing solar UV-B radiation flux in alpine regions. Science, 248, 206-208 (1990).
  • 2) Gleason, J. K., Bhartia, P. K., Herman, J. R., McPeters, R., Newman, P., Stolarski, R. S., Flynn, L., Lawbow, G., Larko, D., Seftor, C., Wellenmeyer, C., Komhyr, D., Miller, A. J., and Planet, W., Record low global ozonein 1992. Science, 260, 523-526 (1993).
  • 3) Mitchell, D. L., The relative cytotoxicity of (6-4) photoproducts and cyclobutane dimers in mammalian cells. Photochem. Photobiol., 48, 51-57 (1988).
  • 4) Sancar, A., Structure and function of DNA photolyase. Biochemistry, 33, 2-9 (1994).
  • 5) Park, H-W., Kim, S., Sancar, A., and Deisenhofer, J., Crystal structure of DNA photolyase from Escherichia coli. Science, 268, 1868-1872 (1995).
  • 6) Hearst, J. E., The structure of photolyase: using photon energy for DNA repair. Science, 268, 1858-1859 (1995).
  • 7) Kim, S-T., Heelis, P. F., Okumura, T., Hirata, Y., Mataga, N., and Sancar, A., Detection of rate and yields of interchromophore (folate→flavin) energy transfer and intermolecular (flavin→DNA) electron transfer in Escherichia coli photolyase by time-resolved fluorescence and absorption spectroscopy. Biochemistry, 30, 11262-11270 (1991).
  • 8) Kim, S.-T., Heelis, K., and Sancar, A., Energy transfer (Deazaflavin→FADH2) and electron transfer (FADH2→T<>T) kinetics in Anacystis nidulans photolyase. Biochemistry, 31, 11244-11248 (1992).
  • 9) Britt, A. B., DNA damage and repair in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol., 47, 75-100 (1996).
  • 10) Langer, B., and Wellmann, E., Phytochrome induction of photoreactivating enzyme in Phaseolus vulgaris L. seedlings. Photochem. Photobiol., 52, 861-863 (1990).
  • 11) Pang, Q., and Hays, J. B., UV-B-inducible and temperature-sensitive photoreactivation of cyclobutane pyrimidine dimers in Arabidopsis thaliana. Plant Physiol., 95, 536-543 (1991).
  • 12) Buchholz, G., Ehmann, B., and Wellmann, E., Ultraviolet light inhibition of phytochrome-induced flavonoid biosynthesis and DNA photolyase formation in mustard cotyledons (Sinapis alba L.). Plant Physiol., 108, 227-234 (1995).
  • 13) Hada, M., Buchholz, G., Hashimoto, T., Nikaido, O., and Wellmann, E., Photoregulation of DNA photolyases in broom Sorghum seedlings. Photochem. Photobiol., 69, 681-685 (1999).
  • 14) Ahmad M., Jarillo, J. A., Klimczak, L. J., Landry, L. G., Peng, T., Last R. L., and Cashmore, A. R., An enzyme similar to animal type II photolyases mediates photoreactivation in Arabidopsis. Plant Cell, 9, 199-207 (1997).
  • 15) Nakajima, S., Sugiyama, M., Iwai, S., Hitomi, K., Otoshi, E., Kim, S-T., Jiang, C-Z., Todo, T., Britt, A. B., and Yamamoto, K., Cloning and characterization of gene (UVR3) required for photorepair of 6-4 photoproducts in Arabidopsis thaliana. Nucleic Acids Res., 26, 638-644 (1998).
  • 16) Hada, M., Hashimoto, T., Nikaido, O., and Shin, M., UVB-induced DNA damage and its photorepair in nuclei and chloroplasts of Spinacia oleracea L. Photochem. Photobiol., 68, 319-322 (1998).
  • 17) Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., and Klenk, D. C., Measurement of protein using bicinchoninic acid. Anal. Biochem., 150, 76-85 (1985).
  • 18) Wiechelman, K., Braun, R., and Fitzpatrick, J., Investigation of the bicinchoninic acid protein assay: identification of the groups responsible for color formation. Anal Biochem., 175, 231-237 (1988).
  • 19) Klocker, H., Auer, B., Burtscher, H. J., Hirsch-Kaufmann, M., and Schweiger, M., A synthetic hapten for induction of thymine dimer specific antibodies. Eur. J. Biochem., 142, 313-316 (1984).
  • 20) Doyle, J. F., and Doyle, J. L., Isolation of plant DNA from fresh tissue. Focus, 12, 13-15 (1990).
  • 21) Mori, T., Nakane, M., Hattori, T., Matsunaga, T., Ihara M., and Nikaido, O., Similataneous establishment of monoclonal antibodies specific for either cyclobutane pyrimidine dimer or (6-4) photoproduct from the same mouse immunized with ultraviolet-irradiated DNA. Photochem. Photobiol., 54, 225-232 (1991).

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