88
Views
12
CrossRef citations to date
0
Altmetric
Original Articles

Preparative 2′-Reduction of ATP Catalyzed by Ribonucleotide Reductase Purified by Liquid-Liquid Extraction

, &
Pages 1836-1841 | Received 07 Feb 2000, Accepted 27 Apr 2000, Published online: 22 May 2014

  • 1) Ludwig, W. and Follmann, H., The specificity of ribonucleoside triphosphate reductase. Eur. J. Biochem., 82, 393-403 (1978).
  • 2) Licht, S., Gerfen, G. J., and Stubbe, J., Thiyl radicals in ribonucleotide reductases. Science, 271, 477-481 (1996).
  • 3) Booker, S., Licht, S., Broderick, J., and Stubbe, J., Coenzyme B12-dependent ribonucleotide reductase: evidence for the participation of five cysteine residues in ribonucleotide reduction. Biochemistry, 33, 12676-12685 (1994).
  • 4) Brunella, A. and Ghisalba, O., Recombinant Lactobacillus leichmannii ribonucleosidetriphosphate reductase as biocatalyst in the preparative synthesis of 2′-deoxyribonucleoside-5′-triphosphates. J. Mol. Catal. B: Enzymatic, in press.
  • . 1986.
  • 6) Hustedt, H., Kroner, K. H., Menge, U., and Kula, M.-R., Protein recovery using two-phase systems. Trends in Biotechnology, 3, 139-144 (1985).
  • 7) Booker, S. and Stubbe, J., Cloning, sequencing, and expression of the adenosylcobalamin-dependent ribonucleotide reductase from Lactobacillus leichmannii. Proc. Natl. Acad. Sci. USA, 90, 8352-8356 (1993).
  • 8) Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254 (1976).
  • . 1982. p. 73- 118.
  • 10) Stevens, R., Stevens, L., and Price, N. C., The stabilities of various thiol compounds used in protein purifications. Biochemical Education, 11, 70 (1983).

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.