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Original Articles

Staurosporine Promotion of Formation of Continuous Monolayers of Primary Rat Hepatocytes by Improving Attachment and Spreading

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Pages 1985-1987 | Received 01 Mar 2000, Accepted 08 May 2000, Published online: 22 May 2014

  • 1) Omura, S., Sasaki, Y., Iwai, Y., and Takeshima, H., Staurosporine, a potentially important gift from a microorganism. J. Antibiot. (Tokyo), 48, 535-548 (1995).
  • 2) Hidaka, H. and Kobayashi, R., Pharmacology of protein kinase inhibitors. Annu. Rev. Pharmacol. Toxicol., 32, 377-397 (1992).
  • 3) Seglen, P. O., Preparation of isolated rat liver cells. Methods Cell Biol., 13, 29-83 (1976).
  • 4) Oka, M., Maeda, S., Koga, N., Kato, K., and Saito, T., A modified colorimetric MTT assay adapted for primary cultured hepatocytes: application to proliferation and cytotoxicity assays. Biosci. Biotechnol. Biochem., 56, 1472-1473 (1992).
  • 5) Lin, K. H., Maeda, S., and Saito, T., Long-term maintenance of liver-specific functions in three-dimensional culture of adult rat hepatocytes with a porous gelatin sponge support. Biotechnol. Appl. Biochem., 21, 19-27 (1995).
  • 6) Yoshimura, M., Nishikawa, A., Nishiura, T., Ihara, Y., Kanayama, Y., Matsuzawa, Y., and Taniguchi, N., Cell spreading in Colo 201 by staurosporin is α3β1 integrin-mediated with tyrosine phosphorylation of Src and tensin. J. Biol. Chem., 270, 2298-2304 (1995).
  • 7) Yamazaki, Y., Sanokawa, R., Fujita, Y., Zhou, D., Kawasaki, K., Tanaka, H., Komatsu, T., Nagasawa, T., Oka, S., Cytoplasmic elongation and rupture in megakaryoblastic leukemia cells via activation of adhesion and motility by staurosporine on fibronectin-bound substratum. J. Cell. Physiol., 179, 179-192 (1999).
  • 8) Handler, J. S., Overview of epithelial polarity. Ann. Rev. Physiol., 51, 729-740 (1989).
  • 9) Moghe, P. V., Berthiaume, F., Ezzell, R. M., Toner, M., Tompkins, R. G., and Yarmush, M. L., Culture matrix configuration and composition in the maintenance of hepatocyte polarity and function. Biomaterials, 17, 373-385 (1996).
  • 10) Musat, A. I., Sattler, C. A., Sattler, G. L., and Pitot, H. C., Reestablishment of cell polarity of rat hepatocytes in primary culture. Hepatology, 18, 198-205 (1993).

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