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Original Articles

In Vitro Biosynthesis of Homogalacturonan by a Membrane-bound Galacturonosyltransferase from Epicotyls of Azuki Bean

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Pages 1519-1527 | Received 15 Dec 2000, Accepted 19 Mar 2001, Published online: 22 May 2014

  • 1) Fry, S.C., Wall polymers: chemical characterisation. In “The growing plant cell wall: chemical and metabolic analysis”, Longman Scientific & Technical, Essex, pp. 102-187 (1988).
  • 2) Bacic, A., Harris, P.J., and Stone, B.A., Structure and function of plant cell walls. In “The biochemistry of plants”, ed. Preiss, J., vol. 14, Academic Press, London, pp. 297-371 (1988).
  • 3) O’Neill, M., Albersheim, P., and Darvill, A., The pectic polysaccharides of primary cell walls. In “Methods in plant biochemistry”, ed. Dey, P.M., vol. 2, Academic Press, London, pp. 415-491 (1990).
  • 4) Zhang, G.F. and Staehelin, L.A., Functional compartmentation of the Golgi apparatus of plant cells. Plant Physiol., 99, 1070-1083 (1992).
  • 5) Ericson, M.C. and Elbein, A.D., Biosynthesis of cell wall polysaccharides and glycoproteins. In “The biochemistry of plants”, ed. Preiss, J., vol. 3, Academic Press, London, pp. 589-616 (1980).
  • 6) Stoddart, R.W., Polysaccharide assembly in eukaryotes. In “The biosynthesis of polysaccharides”, Croom Helm, London, pp. 179-261 (1984).
  • 7) Bourlard, T., Schaumann-Gaudinet, A., Bruyant-Vannier, M.-P., and Morvan, C., Various pectin methyltransferase activities with affinity for low and highly methylated pectins. Plant Cell Physiol., 38, 259-267 (1997).
  • 8) Goubet, F. and Mohnen, D., Solubilization and partial characterization of homogalacturonan-methyltransferase from microsomal membranes of suspension-cultured tobacco cells. Plant Physiol., 121, 281-290 (1999).
  • 9) Ishikawa, M., Kuroyama, H., Takeuchi, Y., and Tsumuraya, Y., Characterization of pectin methyltransferase from soybean hypocotyls. Planta, 210, 782-791 (2000).
  • 10) Pauly, M. and Scheller, H.V., O-Acetylation of plant cell wall polysaccharides: identification and partial characterization of a rhamnogalacturonan O-acetyl-transferase from potato suspension-cultured cells. Planta, 210, 659-667 (2000).
  • 11) Villemez, C.L., Lin, T.-Y., and Hassid, W.Z., Biosynthesis of the polygalacturonic acid chain of pectin by a particulate enzyme preparation from Phaseolus aureus seedlings. Proc. Natl. Acad. Sci. USA, 54, 1626-1632 (1965).
  • 12) Villemez, C.L., Swanson, A.L., and Hassid, W.Z., Properties of polygalacturonic acid-synthesizing enzyme system from Phaseolus aureus seedlings. Arch. Biochem. Biophys., 166, 446-452 (1966).
  • 13) Lin, T.-Y., Elbein, A.D., and Su, J.-C., Substrate specificity in pectin synthesis. Biochem. Biophys. Res. Commun., 22, 650-657 (1966).
  • 14) Bolwell, G.P., Dalessandro, G., and Northcote, D.H., Decrease of polygalacturonic acid synthase during xylem differentiation in sycamore. Phytochemistry, 24, 699-702 (1985).
  • 15) Doong, R.L., Liljebjelke, K., Fralish, G., Kumar, A., and Mohnen, D., Cell-free synthesis of pectin: identification and partial characterization of polygalacturonate 4-α-galacturonosyltransferase and its products from membrane preparations of tobacco cell-suspension cultures. Plant Physiol., 109, 141-152 (1995).
  • 16) Doong, R.L. and Mohnen, D., Solubilization and characterization of a galacturonosyltransferase that synthesizes the pectic polysaccharide homogalacturonan. Plant J., 13, 363-374 (1998).
  • 17) Mohnen, D., Biosynthesis of pectins and galactomannans. In “Comprehensive natural products chemistry”, ed. Pinto, B.M., vol. 3, Elsevier, Amsterdam, pp. 497-527 (1999).
  • 18) Komae, K., Komae, A., and Misaki, A., A 4,5-unsaturated low molecular oligogalacturonide as a potent phytoalexin-elicitor isolated from polygalacturonide of Ficus awkeotsang. Agric. Biol. Chem., 54, 1477-1484 (1990).
  • 19) Park, J.T. and Johnson, M.J., A submicrodetermination of glucose. J. Biol. Chem., 181, 149-151 (1949).
  • 20) Galambos, J.T., The reaction of carbazole with carbohydrates. I. Effect of borate and sulfamate on the carbazole color of sugars. Anal. Biochem., 19, 119-132 (1967).
  • 21) Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254 (1976).
  • 22) Komae, K. and Misaki, A., Isolation and characterization of the gel forming polygalacturonide from seeds of Ficus awkeotsang. Agric. Biol. Chem., 53, 1237-1245 (1989).
  • 23) Adams, G.A., Complete acid hydrolysis. Methods Carbohydr. Chem., 5, 269-276 (1965).
  • 24) Mitcham, E.J., Gross, K.C., and Wasserman, B.P., Synthesis of uridinediphospho-[U-14C]-D-galacturonic acid by enzyme particulate fractions and purification via high performance liquid chromatography. Phytochem. Anal., 2, 112-115 (1991).
  • 25) Liljebjelke, K., Adolphson, R., Baker, K., Doong, R. L., and Mohnen, D., Enzymatic synthesis and purification of uridine diphosphate [14C]galacturonic acid: a substrate for pectin biosynthesis. Anal. Biochem., 225, 296-304 (1995).
  • 26) Ehrlich, F. and Schubert, F., Über die Chemie der Pektinstoffe: Tetra-galakturonsäuren und d-Galakturonsäure aus dem Pektin der Zuckerrübe. Ber. Dtsch. Chem. Ges., 62, 1974-2027 (1929).
  • 27) Vannier, M.P., Thoiron, B., Morvan, C., and Demarty, M., Localization of methyltransferase activities throughout the endomembrane system of flax (Linum usitatissimum L) hypocotyls. Biochem. J., 286, 863-868 (1992).
  • 28) Goubet, F., Council, L.N., and Mohnen, D., Identification and partial characterization of the pectin methyltransferase “homogalacturonan-methyltransferase” from membranes of tobacco cell suspensions. Plant Physiol., 116, 337-347 (1998).
  • 29) Scheller, H.V., Doong, R.L., Ridley, B.L., and Mohnen, D., Pectin biosynthesis: a solubilized α 1,4-galacturonosyltransferase from tobacco catalyzes the transfer of galacturonic acid from UDP-galacturonic acid onto the non-reducing end of homogalacturonan. Planta, 207, 512-517 (1999).

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