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Original Articles

Protein Engineering of Novel Proteinase Inhibitors and Their Effects on the Growth of Spodoptera exigua Larvae

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Pages 2259-2264 | Received 18 Apr 2001, Accepted 01 Jun 2001, Published online: 22 May 2014

  • 1) Green, T.R. and Ryan, C., Wound-induced proteinase inhibitors in plant leaves: a possible defense mechanism against insects. Science, 175, 776-777 (1972).
  • 2) Broadway, R.M. and Duffey, S.S., Plant proteinase inhibitors: mechanism of action and effect on the growth and digestive physiology of larval Heliothis zea and Spodoptera exigua. J. Insect Physiol., 32, 827-833 (1986).
  • 3) Johnson, R., Narvaez, J., An, G., and Ryan, C., Expression of proteinase inhibitors I and II in transgenic tobacco plants: effects on natural defense against Manduca sexta larvae. Proc. Natl. Acad. Sci. USA., 86, 9871-9875 (1989).
  • 4) Hilder, V.A., Gatehouse, A.M.R., Sheerman, S.E., Barker, R.F., and Boulter, D., A novel mechanism of insect resistance engineered into tobacco, Nature, 330, 160-163 (1987).
  • 5) Jongsma, M.A., Bakker, P.L., Peters, J., Bosch, D., and Stiekema, W.J., Adaptation of Spodoptera exigua larvae to plant proteinase inhibitors by induction of gut proteinase activity insensitive to inhibition. Proc. Natl. Acad. Sci. USA., 92, 8041-8045 (1995).
  • 6) Kondo, H., Abe, K., Nishimura, I., Watanabe, H., Emori, Y., and Arai, S., Two distinct cystatin species in rice seeds with different specificities against cysteine proteinases. J. Biol. Chem., 265, 15832-15837 (1990).
  • 7) Kouzuma, Y., Inanaga, H., Doi-Kawano, K., Yamasaki, N., and Kimura, M., Molecular cloning and functional expression of cDNA encoding the cysteine proteinase inhibitor with three cystatin domains from sunflower seeds. J. Biochem., 128, 161-166 (2000).
  • 8) Waldron, C., Wegrich, L.M., Merlo, P.A.O., and Walsh, T.A., Characterization of a genomic sequence coding for potato multicystatin, an eight-domain cysteine proteinase inhibitor. Plant Mol. Biol., 23, 801-812 (1993).
  • 9) Orr, G.L., Strickland, J.A., and Walsh, T.A., Inhibition of Diabrotica larval growth by a multicystatin from potato tubers. J. Insect Physiol., 40, 839-900 (1994).
  • 10) Miura, S. and Funatsu, G., Isolation and amino acid sequences of two trypsin inhibitors from the seeds of bitter gourd (Momordica charantia). Biosci. Biotechnol. Biochem., 59, 469-473 (1995).
  • 11) Studier, F.W., Rosenberg, A.H., Dunn, J.J., and Dubendorff, J.W., Use of T7 RNA polymerase to direct expression of cloned gene. Methods Enzymol., 185, 60-89 (1990).
  • 12) Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
  • 13) Kouzuma, Y., Yamasaki, N., and Kimura, M., The tissue-type plasminogen activator inhibitor ETIa from Erythrina variegata: structural basis for the inhibitory activity by cloning, expression, and mutagenesis of the cDNA encoding ETIa. J. Biochem., 121, 456-463 (1997).
  • 14) Henderson, P.J.F., A linear equation that describes the steady-state kinetics of enzymes and subcellular particles interacting with tightly bound inhibitors. Biochem. J., 127, 321-333 (1972).
  • 15) Okada, M., Mass production method of the Spodoptera litura nuclear polyhedrosis virus. Plant Protection, 38, 362-365 (1984).
  • 16) Ignoffo, C.M., The nuclear polyhedrosis of Heliothis zea (Boddie) and Heliothis virescence (Fabricis). IV. Bioassay of virus activity. J. Invertebr. Pathol., 7, 315-319 (1965).
  • 17) Bode, W. and Huber, R., Natural proteinase inhibitors and their interaction with proteinases. Eur. J. Biochem., 204, 433-451 (1992).
  • 18) Koiwa, H., Shade, R.E., Zhu-Salzman, K., D’Urzo, M.P., Murdock, L.L., Bressan, R.A., and Hasegawa, P.M., A plant defensive cystatin (soyacystatin) targets cathepsin L-like digestive cysteine proteinases (DvCALs) in the larval midgut of western corn rootworm (Diabrotica virgifera). FEBS Lett., 471, 67-70 (2000).
  • 19) Gutierrez-Campos, R., Torres-Acosta, J.A., Saucedo-Arias, L.J., and Gomez-Lim M.A., The use of cysteine proteinase inhibitors to engineer resistance against potyviruses in transgenic tobacco plants. Nat. Biotech., 17, 1223-1226 (1999).

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