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Bioscience, Biotechnology, and Biochemistry Volume 65, 2001 - Issue 2
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Original Articles

Construction of a Chimeric Shuttle Plasmid via a Heterodimer System: Secretion of an scFv Protein from Bacillus brevis Cells Capable of Inhibiting Hemagglutination

Teruaki SHIROZADepartment of Biochemistry, Nihon University School of Dentistry at Matsudo
,
Yasuko SHIBATADepartment of Biochemistry, Nihon University School of Dentistry at Matsudo
,
Mitsuo HAYAKAWADepartment of Biochemistry, Nihon University School of Dentistry at Matsudo
,
Noriko SHINOZAKIDepartment of Microbiology, Nihon University School of Dentistry at Matsudo
,
Kazuo FUKUSHIMADepartment of Microbiology, Nihon University School of Dentistry at Matsudo
,
Shigezo UDAKADepartment of Fermentation Science, Tokyo University of Agriculture
&
Yoshimitsu ABIKODepartment of Biochemistry, Nihon University School of Dentistry at Matsudo
 show all
Pages 389-395 | Received 24 Aug 2000, Accepted 02 Oct 2000, Published online: 22 May 2014

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  • 2) Udaka, S. and Yamagata, H., High level secretion of heterologous proteins by Bacillus brevis. Methods Enzymol., 217, 23-33 (1993).
  • 3) Shiroza, T. and Kuramitsu, H.K., Construction of a model secretion system for oral streptococci. Infect. Immun., 61, 3745-3755 (1993).
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  • 7) Shiroza, T., Shinozaki, N., Fukushima, K., and Abiko, Y., Transformation of oral streptococci with single-stranded DNA plasmids and their compatibility. Anal. Biochem., 253, 132-134 (1997).
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  • 15) Sagiya, Y., Yamagata, H., and Udaka, S., Direct high-level secretion into the culture medium of tuna growth hormone in a biologically active form by Bacillus brevis. Appl. Microbiol. Biotechnol., 42, 358-363 (1994).
  • 16) Takimura, Y., Kato, M., Ohta, T., Yamagata, H., and Udaka, S., Secretion of human interleukin-2 in a biologically active form by Bacillus brevis directly into the culture medium. Biosci. Biotechnol. Biochem., 61, 1858-1861 (1997).
  • 17) Inoue, Y., Ohta, T., Tada, H., Iwase, S., Udaka, S., and Yamagata, H., Efficient production of a functional mouse/human chimeric Fab' against human urokinase-type plasminogen activator by Bacillus brevis. Appl. Microbiol. Biotechnol., 48, 487-492 (1997).

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