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Bioscience, Biotechnology, and Biochemistry Volume 66, 2002 - Issue 5
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Original Articles

Analysis of Hydrolytic Activity of a 65-kDa Chitinase from the Silkworm, Bombyx mori

Babiker M. A. ABDEL-BANATLaboratory of Biochemistry, Department of Biological Science, Faculty of Agriculture, Yamaguchi University Yamaguchi 753-8515, Japan
,
Weiwen ZHOULaboratory of Biochemistry, Department of Biological Science, Faculty of Agriculture, Yamaguchi University Yamaguchi 753-8515, Japan
,
Shuji KARASUDALaboratory of Biochemistry, Department of Biological Science, Faculty of Agriculture, Yamaguchi University Yamaguchi 753-8515, Japan
&
Daizo KOGALaboratory of Biochemistry, Department of Biological Science, Faculty of Agriculture, Yamaguchi University Yamaguchi 753-8515, Japan
Pages 1119-1122 | Received 15 Oct 2001, Accepted 21 Jan 2002, Published online: 22 May 2014

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  • 2) Abdel/Banat, B. M. A., Kameyama Y., Yoshioka T., Koga D., Purification and characterization of a 54 kDa chitinase from Bombyx mori.Insect Biochem. Mol. Biol., 29, 537-547 (1999).
  • 3) Koga, D., Fujimoto, H., Funakoshi, T., Utsumi, T., and Ide, A., Appearance of chitinolytic enzymes in integument of Bombyx mori during the larval-pupal transformation. Evidence for zymogenic forms. Insect Biochem., 19, 123-128 (1989).
  • 4) Koga, D., Mai, M. S., Dziadik-Turner, C., Kramer, K. J., Kinetics and mechanism of exochitinase from the tobacco hornworm, Manduca sexta L. (Lepidoptera: Sphingidae). Insect Biochem., 12, 493-499 (1982).
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  • 6) Koga, D., Funakoshi, T., Mizuki, K., Ide, A., Kramer, K. J., Zen, K. C., Choi, H., and Muthukrishnan, S., Immunoblot analysis of chitinolytic enzymes in integument and molting fluid of the silkworm, Bombyx mori, and the tobacco hornworm, Manduca sexta.Insect Biochem. Mol. Biol., 22, 305-311 (1992).
  • 7) Abdel-Banat, B. M. A., Koga, D., A genomic clone for a chitinase gene from the silkworm, Bombyx mori: structural organization identifies functional motifs. Insect Biochem. Mol. Biol., 31, 497-508 (2001).
  • 8) Choi, H.-K., Choi, K.-H., Kramer, K. J., and Muthukrishnan, S., Isolation and characterization of a genomic clone for the gene of an insect molting enzyme, chitinase. Insect Biochem. Mol. Biol., 27, 37-47 (1997).
  • 9) Kramer, K. J., Corpuz, L. M., Choi, H., and Muthukrishnan, S., Sequence of a cDNA and expression of the gene encoding epidermal and gut chitinase of Manduca sexta.Insect Biochem. Molec. Biol., 23, 691-701 (1993).
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  • 11) Brameld, K. A. and Goddard III, W. A., Substrate distortion to a boat conformtion at subsite-1 is critical in the mechanism of family 18 chitinases. J. Am. Chem. Soc., 120, 3571-3580 (1998).
  • 12) Terwisscha Van Scheltinga, A. C., Kalk, K. H., Beintema, J. J., and Dijkstra, B. W., Crystal structures of hevamine, a plant defense protein with chitinase and lysozyme activity, and its complex with an inhibitor. Structure, 2, 1169-1180 (1994).
  • 13) Terwisscha Van Scheltinga, A. C., Armand, S., Kalk, K. H., Isogai, A., Henrissat, B., and Dijkstra, B. W., Stereochemistry of chitin hydrolysis by a plant chitinase/lysozyme and X-ray structure of a complex with allosamidin: evidence for substrate-assisted catalysis. Biochem., 34, 15619-15623 (1995).
  • 14) Tews, I., Terwisscha Van Scheltinga, A. C., Perrakis, A., Wilson, K. S., and Dijkstra, B. W., Substrate- assisted catalysis unifies two families of chitinolytic enzymes. J. Am. Chem. Soc., 119, 7954-7959 (1997).
  • 15) Koga, D., Mitsutomi, M., Kono, M., and Matsumiya, M., Biochemistry of chitinases. Chitin and chitinases. Edited by P. Jolles and R. A. A., Muzzarelli, pp. 111-123, Birkhauser Publishing Ltd. Basel, Switzerland (1999).
  • 16) Koga, D., Yoshioka, T., and Arakane, Y., HPLC analysis of anomeric formation and cleavage pattern by chitinolytic enzyme. Biosci. Biotechnol. Biochem., 62, 1643-1646 (1998).
  • 17) Huang, X., Zhang, H., Zen, K.-C., Muthukrishnan, S., and Kramer, K. J., Homology modeling of the insect chitinase catalytic domain-oligosaccharide complex and the role of a putative active site tryptophan in catalysis. Insect Biochem. Mol. Biol., 30, 107-117 (2000).
  • 18) Koga, D., Tsukamoto, T., Sueshige, N., Utsumi, T., and Ide, A., Kinetics of chitinase from yam, Dioscorea opposita Thunb. Agric. Biol. Chem., 53, 3121-3126 (1989).

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