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Bioscience, Biotechnology, and Biochemistry Volume 66, 2002 - Issue 5
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Original Articles

Functional Expression of Chitinase and Chitosanase, and Their Effects on Morphologies in the Yeast Schizosaccharomyces pombe

Kumiko SHIMONODepartment of Life Science and Biotechnology, Faculty of Life & Environmental Science, Shimane University Matsue 690-8504, Japan
,
Hideyuki MATSUDADepartment of Life Science and Biotechnology, Faculty of Life & Environmental Science, Shimane University Matsue 690-8504, Japan
&
Makoto KAWAMUKAIDepartment of Life Science and Biotechnology, Faculty of Life & Environmental Science, Shimane University Matsue 690-8504, Japan
Pages 1143-1147 | Received 12 Nov 2001, Accepted 07 Dec 2001, Published online: 22 May 2014

  • 1) Lipke, P. N. and Ovalle, R., Cell wall architecture in yeast: new structure and new challenges. J. Bacteriol., 180, 3735-3740 (1998).
  • 2) Valdivieso, M. H., Durán, A., and Roncero, C., Chitin synthases in yeast and fungi. in “Chitin and Chitosan”, eds. Jollés, P., and Muzzarelli, R. A. A., Birkhäuser Verlag, Switzerland, pp. 55-69 (1999).
  • 3) Sietsma, J. H. and Wessels, J. G. H., The occurrence of glucosaminoglycan in the wall of Schizosaccharomyces pombe.J. Gener. Microbiol., 136, 2261-2265 (1990).
  • 4) Arellano, M., Cartagena-Lirola, H., Hajibagheri, M. A. N., Duran, A., and Valdivieso, M. H., Proper ascospore maturation requires the chs1+ chitin synthase gene in Schizosaccharomyces pombe.Mol. Microbiol., 35, 79-89 (2000).
  • 5) Forsburg, S. L. and Sherman, D. A., General purpose tagging vectors for fission yeast. Gene, 191, 191-195 (1997).
  • 6) Okada, H., Sekiya, T., Yokoyama, K., Tohda, H., Kumagai, H., and Morikawa, Y., Efficient secretion of Trichoderma reesei cellobiohydrolase II in Schizosaccharomyces pombe and characterization of its products. Appl. Microbiol. Biotechnol., 49, 301-308 (1998).
  • 7) Okada, H., Tada, K., Sekiya, T., Yokoyama, K., Takahashi, A., Tohda, H., Kumagai, H., and Morikawa, Y., Molecular characterization and heterologous expression of the gene encoding a low-molecular-mass endoglucanase from Trichoderma reesei QM9414. Appl. Environ. Microbiol., 64, 555-563 (1998).
  • 8) Tokunaga, M., Kawamura, A., Yonekyu, S., Kishida, M., and Hishinuma, F., Secretion of mouse α-amylase from fission yeast Schizosaccharomyces pombe: presence of chymostatin-sensitive protease activity in the culture medium. Yeast, 9, 379-387 (1993).
  • 9) Draborg, H., Kauppinen, S., Dalbμge, H., and Christgau, S., Molecular cloning and expression in S. cerevisiae of two exochitinases from Trichoderma harzianum. Biochem. Mol. Biol. Int., 36, 781-791 (1995).
  • 10) Draborg, H., Christgau, S., Halkier, T., Rasmussen, G., Dalbøge, H., and Kauppinen, S., Secretion of an enzymatically active Trichoderma harzianum endochitinase by Saccharomyces cerevisiae.Curr. Genet., 29, 404-409 (1996).
  • 11) Park, J. K., Morita, K., Fukumoto, I., Yamasaki, Y., Nakagawa, T., Kawamukai, M., and Matsuda, H., Purification and characterization of the chitinase (ChiA) from Enterobacter sp. G-1. Biosci. Biotechnol. Biochem., 61, 684-689 (1997).
  • 12) Park, J. K., Okamoto, T., Yamasaki, Y., Tanaka, K., Nakagawa, T., Kawamukai, M., and Matsuda, H., Molecular cloning, nucleotide sequencing, and regulation of the chiA gene encoding one of chitinases from Enterobacter sp. G-1. J. Ferment. Bioengin., 84, 493-501 (1997).
  • 13) Park, J. K., Shimono, K., Ochiai, N., Shigeru, K., Kurita, M., Ohta, Y., Tanaka, K., Matsuda, H., and Kawamukai, M., Purification, characterization, and gene analysis of a chitosanase (ChoA) from Matsuebacter chitosanotabidus 3001. J. Bacteriol., 181, 6642-6649 (1999).
  • 14) Guthrie, C. and Fink, G. R., Guide to yeast genetics and molecular biology. Meth. Enzymol., 194, Academic press, New York (1991).
  • 15) Basi, G., Schmid, E., and Maundrell, K., TATA box mutations in the Schizosaccharomyces pombenmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility. Gene, 123, 131-136 (1993).
  • 16) Shimono, K., Shigeru, K., Tsuchiya, A., Itou, N., Ohta, Y., Tanaka, K., Nakagawa1, T., Matsuda, H., and Kawamukai, M., Two glutamic acids in chitosanase A from Matsuebacter chitosanotabidus 3001 are the catalytically important residues. J. Biochem., 131, 87-96 (2002).
  • 17) Imoto, T. and Yagishita, K., A simple activity measurement of lysozyme. Agri. Biol. Chem., 35, 1154-1156 (1971).

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