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Original Articles

Technical Improvement to 2D-PAGE of Rice Organelle Membrane Proteins

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Pages 1170-1173 | Received 03 Dec 2001, Accepted 15 Jan 2002, Published online: 22 May 2014

  • 1) O'Farrell, P. H., High-resolution two-dimensional electrophoresis of proteins. J. Biol. Chem., 250, 4007-4021 (1975).
  • 2) Hirano, H., Varietal differences of leaf protein profiles in mulberry. Phytochemistry, 21, 1513-1518 (1982).
  • 3) Bjellqvist, B., Ek, K., Righetti, P. G., Gianazza, E., Gorg, A., Westermeier, R., and Postel, W., Isoelectric focusing in immobilized pH gradients: principle, methodology and some applications. J. Biochem. Biophys. Methods, 6, 317-339 (1982).
  • 4) Dunbar, B. S., Kimura, H., and Timmons, T. M., Protein analysis using high-resolution two-dimensional polyacrylamide gel electrophoresis. Methods Enzymol., 182, 441-459 (1990).
  • 5) Patton, W. F., Pluskal, M. G., Skea, W. M., Buecker, J. L., Lopez, M. F., Zimmermann, R., Belanger, L. M., and Hatch, P. D., Development of a dedicated two-dimensional gel electrophoresis system that provides optimal pattern reproducibility and polypeptide resolution. BioTechniques, 8, 518-527 (1990).
  • 6) Herbert, B. R., Mollyo, M. P., Gooley, A. A., Walsh, B. J., Bryson, W. G., and Williams, K. L., Improved protein solubility in two-dimensional electrophoresis using tributyl phosphine as reducing agent. Electrophoresis, 19, 845-851 (1998).
  • 7) Komatsu, S., Kajiwara, H., and Hirano, H., A rice protein library: a data-file of rice proteins separated by two-dimensional electrophoresis. Theor. Appl. Genet., 86, 935-942 (1993).
  • 8) Kamo, M., Kawakami, T., Miyatake, N., and Tsugita, A., Separation and characterization of Arabidopsis thaliana proteins by two-dimensional gel electrophoresis. Electrophoresis, 16, 423-430 (1995).
  • 9) Santoni, V., Rouquie, D., Doumas, P., Mansion, M., Boutry, M., Degand, H., Dupree, P., Packman, L., Sherrier, J., Prime, T., Bauw, G., Posada, P., Rouze, P., Dehais, P., Sahnoun, I., Barlier, I., and Rossingol, M., Use of a proteome strategy for tagging proteins present at the plasma membrane. Plant J., 16, 633-641 (1998).
  • 10) Thiellement, H., Bahrman, N., Damerval, C., Plomion, C., Rossignol, M., Santoni, V., de Vienne, D., and Zivy, M., Proteomics for genetic and physiological studies in plants. Electrophoresis, 20, 2013-2026 (1999).
  • 11) Komatsu, S., Rakwal, R., and Li Z., Separation and characterization of proteins in rice (Oryza sativa) suspension cultured cells. Plant Cell Tissue Org. Cul., 55, 183-192 (1999).
  • 12) Peltier, J. B., Firso, G., Kalume, D. E., Roepstoff, P., Nilson, F., Adamska, I., and van Wijl, K. J., Proteomics of the chroloplast: Systematic identification and targeting analysis of lumenal and peripheral thylakoid proteins. Plant Cell, 12, 319-342 (2000).
  • 13) Mikami, S., Hori, H., and Mitsui, T., Separation of distinct compartments of rice Golgi complex by sucrose density gradient centrifugation. Plant Sci., 161, 665-675 (2001).
  • 14) Hurkman, W. J. and Tanaka, C. K., Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis. Plant Physiol., 81, 802-806 (1986).
  • 15) Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
  • 16) Celis, J. E., Rasmussen, H. H., Olsen, E., Madsen, P., Leffers, H., Honnore, B., Dejgaard, K., Gromov, P., Hoffmann, H. J., Nielsen, M., Vassilev, A., Vintermyr, O., Hao, J., Celis, A., Basse, B., Lauridsen, J. B., Ratz, G. P., Andersen, A. H., Kjaergaard, E., Puype, M., Damme, J. V., and Vandekerckhove, J., The human keratinocyte two-dimensional gel protein database: Update 1993. Electrophoresis, 14, 1091-1198 (1993).

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