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Bioscience, Biotechnology, and Biochemistry Volume 66, 2002 - Issue 6
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Original Articles

Stabilization of Flavobacterium meningosepticum Glycerol Kinase by Introduction of a Hydrogen Bond

Shin-ichi SAKASEGAWAAsahi Kasei Corporation Ohito-Cho, Tagata-gun, Shizuoka 410-2321, Japan
,
Hideki TAKEHARAMolecular Gene Technics, Genetic Resource Technology, Kyushu University Fukuoka 812-8581, Japan
,
Issei YOSHIOKAAsahi Kasei Corporation Ohito-Cho, Tagata-gun, Shizuoka 410-2321, Japan
,
Hideo MISAKIAsahi Kasei Corporation Ohito-Cho, Tagata-gun, Shizuoka 410-2321, Japan
,
Haruhiko SAKURABADepartment of Biological Science and Technology, Faculty of Engineering, The University of Tokushima Tokushima 770-8506, Japan
&
Toshihisa OHSHIMADepartment of Biological Science and Technology, Faculty of Engineering, The University of Tokushima Tokushima 770-8506, Japan
Pages 1374-1377 | Received 12 Nov 2001, Accepted 26 Feb 2002, Published online: 22 May 2014

  • 1) Fossati, P. and Prencipe, L., Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin. Chem., 28, 2077-2080 (1982).
  • 2) Sakasegawa, S., Yoshioka, I., Koga, S., Takahashi, M., Matsumoto, K., Misaki, H., and Ohshima, T., A novel glycerol kinase from Flavobacterium meningosepticum: characterization, gene cloning and primary structure. Biosci. Biotechnol. Biochem., 62, 2388-2395 (1998).
  • 3) Comer, M. J., Bruton, C. J., and Atkinson, T., Purification and properties of glycerol kinase from Bacillus stearothermophilus. J. Appl. Biochem., 1, 259-270 (1979).
  • 4) Sakasegawa, S., Takehara, H., Yoshioka, I., Takahashi, M., Kagimoto, Y., Misaki, H., Sakuraba, H., and Ohshima, T., Increasing the thermostability of Flavobacterium meningosepticum glycerol kinase by changing Ser329 to Asp in the subunit interface region. Protein Eng., 14, 663-667 (2001).
  • 5) Hurley, J. H., Faber, H. R., Worthylake, D., Meadow, N. D., Roseman, S., Pettigrew, D. W., and Remington, S. J., Structure of the regulatory complex of Escherichia coli IIIGlc with glycerol kinase. Science, 259, 673-677 (1993).
  • 6) Kunkel, T. A., Roberts, J. D., and Zakour, R. A., Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol., 154, 367-382 (1987).
  • 7) Suzuki, Y., Nakamura, N., Kishigami, T., and Abe, S., Bacillus thermoglucosidius α-glucosidase. Temperature dependence of activity and stability. J. Biochem., 87, 745-751 (1980).
  • 8) Irun, M. P., Maldonado, S., and Sancho, J., Stabilization of apoflavodoxin by replacing hydrogen-bonded charged Asp or Glu residues by the neutral isosteric Asn or Gln. Protein Eng., 14, 173-181 (2001).
  • 9) Thorner, J. W. and Paulus, H., Catalytic and allosteric properties of glycerol kinase from Escherichia coli. J. Biol. Chem., 248, 3922-3932 (1973).
  • 10) Pettigrew, D. W., Yu, G. J., and Liu, Y., Nucleotide regulation of Escherichia coli glycerol kinase: initial-velocity and substrate binding studies. Biochemistry, 29, 8620-8627 (1990).
  • 11) Bystrom, C. E., Pettigrew, D. W., Branchaud, B. P., O'Brien, P., and Remington, S. J., Crystal structures of Escherichia coli glycerol kinase variant S58→W in complex with nonhydrolyzable ATP analogues reveal a putative active conformation of the enzyme as a result of domain motion. Biochemistry, 38, 3508-3518 (1999).
  • 12) Pettigrew, D. W., Inactivation of Escherichia coli glycerol kinase by 5,5′-dithiobis (2-nitrobenzoic acid) and N-ethylmaleimide: evidence for nucleotide regulatory binding sites. Biochemistry, 25, 4711-4718 (1986).

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