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Original Articles

Expression of Nicotiana glutinosa Ribonucleases in Escherichia coli

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Pages 910-912 | Received 18 Oct 2001, Accepted 27 Nov 2001, Published online: 22 May 2014

  • 1) Kariu, T., Sano, K., Shimokawa, H., Itoh. R., Yamasaki, N., and Kimura, M., Isolation and characterization of a wound-inducible ribonuclease from Nicotiana glutinosa leaves. Biosci. Biotechnol. Biochem., 62, 1144-1151 (1998).
  • 2) Kurata, N., Kariu, T., Kawano, S., and Kimura, M., Molecular cloning of cDNAs encoding ribonuclease-related proteins in Nicotiana glutinosa leaves, as induced in response to wounding or to TMV-infection. Biosci. Biotechnol. Biochem., 66, 391-397 (2002).
  • 3) Studier, F. W., Rosenberg, A. H. R., Dunn, J. J., and Dubendorff, J. W., Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol., 185, 60-89 (1990).
  • 4) Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
  • 5) Abel, S. and Glund, K., Localization of RNA-degrading enzyme activity within vacuoles of cultured tomato cells. Physiol. Plant, 66, 79-86 (1986).
  • 6) Numata, T., Kashiba, T., Hino, M., Funatsu, G., Ishiguro, M., Yamasaki, N., and Kimura, M., Expression and mutational analysis of amino acid residues involved in catalytic activity in a ribonuclease MC1 from the seeds of bitter gourd. Biosci. Biotechnol. Biochem., 64, 603-605 (2000).

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