156
Views
17
CrossRef citations to date
0
Altmetric
Original Articles

Gene Cloning and Biochemical Analysis of Thermostable Chitosanase (TCH-2) from Bacillus coagulans CK108

, , , , , & show all
Pages 986-995 | Received 22 Oct 2001, Accepted 18 Jan 2002, Published online: 22 May 2014

  • 1) Arvanitoyannis, I. S., Nakayama, A., and Abia, S., Chitosan and gelatin edible films: state diagrams, mechanical and permeation properties. Carbohydr. Polym., 37, 371-382 (1998).
  • 2) Felse, P. A., and Panda, T., Studies on applications of chitin and its derivatives. Bioprocess Engineering., 20, 505-512 (1999).
  • 3) Li, Q., Dunn, E. T., Grandmaison, E. W., and Goosen, M. F. A., Applications and properties of chitosan, J. Bioactive and Compatible Polymers., 7, 370-397 (1992).
  • 4) Murata, J., Ohya, Y., and Ouchi, T., Possibility of application of quaternary chitosan having pendant galactose residues as gene delivery tools. Carbohyd. Polym., 29, 69-74 (1996).
  • 5) Fenton, D. M., and Eveleigh, D. E., Purification and mode of action of a chitosanase from Penicillium islandicum.J. Gen. Microbiol., 126, 151-165 (1981).
  • 6) Yoshinara, K., Hosokawa, J., Kubo, T., Nishiyama, M., and Koba. Y., Purification and properties of chitosanase from Pseudomonas sp. H-14. Biosci. Biotechnol. Biochem., 56, 972-973 (1992).
  • 7) Tominaga, Y. and Tsujisaka. Y., Purification and some enzymatic properties of the chitosanase from Bacillus R-4 which lysis Rhizopus cell walls. Biochim. Biophys. Acta, 410, 145-155 (1975).
  • 8) Parro, V., Roman, M. S., Galindo, I., Purnelle, B., Bolotin, A., Sorokin, A., and Mellado, R. P., A 23911 bp region of the Bacillus subtilis genome comprising genes located upstream and downstream of the lev operon. Microbiology, 143, 1321-1326 (1997).
  • 9) Yamada, T., Hiramatsu, S., Songsri, P., and Fujie, M., Alternative expression of a chitosanase gene produces two different proteins in cells infected with Chlorella virus CVK2. Virology, 14, 361-368 (1997).
  • 10) Yoon, H. G., Kim, H. Y., Lim, Y. H., Kim, H. K., Shin, D. H., Hong, B. S., and Cho, H. Y., Thermostable Chitosanase from Bacillus sp. CK4: Cloning, Expression of the Gene, and Characterization of the Enzyme. App.l Environ. Microbiol., 66, 3727-3734 (2000).
  • 11) Fukamizo, T., and Brzezinski, R., Chitosanase from Streptomyces sp. strain N174: a comparative review of its structure and function. Biochem. Cell Biol., 75, 687-696 (1997).
  • 12) Fukamizo, T., Honda, Y., Goto, S., Boucher, I., and Brzezinski, R., Reaction mecha- nism of chitosanase from Streptomyces sp. strain N174. Biochem. J., 311, 377-383 (1995).
  • 14) Seino, H., Tsuluda. K., and Shimasue. Y., Properties and action patterns of a chitosanase from Bacillus sp. PI-7S. Agric. Biol. Chem., 55, 2421-2423 (1991).
  • 15) Akiyama, K., Fujita, T., Kuroshima, K., Sakane, T., Yokota, A., and Takata, R., Purification and gene cloning of a chitosanase from Bacillus ehemensis EAG1. J. Biosci. Bioeng. 87, 383-385 (1999).
  • 17) Rivas, L. A., Parro, V., Moreno-Paz, M., and Mellado, R. P., The Bacillus subtilis 168 csn gene encodes a chitosanase with similar properties to a Streptomyces enzyme.Microbiology., 146, 2929-36 (2000).
  • 18) Masson, J. Y., Boucher, I., Neugebauer, W. A., Ramotar, D., and Brzezinski, R., A new chitosanase gene from a Nocardioides sp. is a third member of glycosyl hydrolase family 46. Microbiology., 141, 2629-2635 (1995).
  • 19) Shimosaka, M., Fukumori, Y., Zhang, X. Y., He, N. J., Kodaira, R., and Okazaki, M., Molecular cloning and characterization of a chitosanase from the chitosanolytic bacterium Burkholderia gladioli strain CHB101. Appl. Microbiol. Biotechnol., 54, 354-360 (2000).
  • 20) Marcotte, E. M., Monzingo, A. F., Ernst, S. R., Brzezinski, R., and Robertus, J. D., X-ray structure of an anti-fungal chitosanase from Streptomyces sp. N174. Nat. Struct. Biol., 3, 155-162 (1996).
  • 21) Saito, J., Kita, A., Higuchi, Y., Nagata, Y., Ando, A., and Miki, K., Crystal structure of chitosanase from Bacillus circulans MH-K1 at 1.6-A resolution and its substrate recognition mechanism. J. Biol. Chem., 274, 30818-30825 (1999).
  • 22) Yoon, H. G., Ha, S. C., Lim, Y. H., and Cho, H. Y., New thermostable chitosanase from Bacillus sp.: Purification and characterization. J. Microbiol. Biotechnol., 8, 449-454 (1998).
  • 23) Uchida, Y., and Ohtakara, A., Chitosanase from the Bacillus species. Methods. Enzymol., 161, 501-505 (1989).
  • 24) Hsu, S. C., and Lockwood, L., Powdered chitin agar as a selective medium for enumeration of Actinomycetes in water soil. Appl. Microbiol. 29, 422-426 (1975).
  • 25) Miller, L., Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem., 31, 426-431 (1959).
  • 26) Lowry, O. H., Rosebrough, N. J., Fan, A. L., and Randall, R. J., Protein measurment with the folin phenol reagent. J. Biol. Chem., 193, 265-271 (1951).
  • 27) Kagawa, Y., Nojima, H., Nukiwa, N., Ishizuka, M., Nakajima, T., Yasuhara, T., Tanaka, T., and Oshima, T., High guanine plus cytosine content in the third letter of codons of an extreme thermophile. DNA sequence of the isopropylmalate dehydrogenase of Thermus thermophilus.J. Biol. Chem., 259, 2956-2960 (1984).
  • 28) Boucher, I., Fukamizo, T., Honda, Y., Willick, G. E., Neugebauer, W. A., and Brzezinski, R., Site-directed mutagenesis of evolutionary conserved carboxylic amino acids in the chitosanase from Streptomyces sp. N174: Reaveals two residues essential for catalysis. J. Biol. Chem., 270, 31077-31082 (1995).
  • 29) Vieille, C., Burdette, D. S., and Zeikus, J. G., Thermozymes. Biotechnol. Annu. Rev., 2, 1-83 (1996).
  • 30) Lepock, J. R., Frey, H. E., and Hallewell, R. A., Contribution of conformational stability and reversibility of unfolding to the increased thermostability of human and bovine superoxide dismutase mutated at free cysteines. J. Biol. Chem., 265, 21612-21618 (1990).
  • 31) Katsuyuki, T., Ohshima, A., Schsidegger, A., Inagaki, K., Tanaka, H., and Soda, K., Thermostable alanine racemase from Bacillus stearothermophilus: DNA and protein sequence determination and secondary structure prediction. Biochemistry., 27, 1311-1316 (1988).
  • 32) Zuber, H., Thermophily. Basic Life Sci., 18, 499-512 (1981).
  • 13) Yoon, H. G., Kim, H. Y., Kim, H. K., Hong, B. S., Shin, D. H., and Cho, H. Y., Thermostable chitosanase from Bacillus sp. strain CK4: its purification, characterization, and reaction patterns. Biosci. Biotechnol. Biochem., 65, 802-809 (2001).
  • 16) Zhang, X. Y., Dai, A. L., Kuroiwa, K., Kodaira, R., Nogawa, M., Shimosaka, M., and Okazaki, M., Cloning and characterization of a chitosanase gene from the koji mold Aspergillus oryzae strain IAM 2660. Biosci. Biotechnol. Biochem., 65, 977-981 (2001).

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.