315
Views
44
CrossRef citations to date
0
Altmetric
Original Articles

Development of an Effective Sample Preparation Method for the Proteome Analysis of Body Fluids Using 2-D Gel Electrophoresis

, &
Pages 1574-1577 | Received 18 Nov 2002, Accepted 25 Mar 2003, Published online: 22 May 2014

  • 1) Wataral, H., Inagakl, Y., Kubota, N., Fuju, K., Nagafune, J., Yamaguchi, Y., and Kadoya, T., Proteomic approach to the identification of cell membrane proteins. Electrophoresis, 21, 460-464 (2000).
  • 2) Alaiya, A. A., Franzen, B., Auer, G., and Linder, S., Cancer proteomics: From identification of novel markers to creation of artificial learning models for tumor classification. Electrophoresis, 21, 1210-1217 (2000).
  • 3) Hochstrasser, D. F., Clinical and biomedical applications of proteomics, Proteome research: New frontiers in functional genomics, chapter 8, Springer-Verlag (1997).
  • 4) Rabilloud, T., Solubilization of proteins for electrophoretic analyses. Electrophoresis, 17, 813-829 (1996).
  • 5) Herbert, B., Advances in protein solubilization for two-dimensional electrophoresis. Electrophoresis, 20, 660-663 (1999).
  • 6) Southan, C., Cutler, P., Birrell, H., Connell, J., Fantom, K. G., Sims, M., Shaikh, N., and Schneider, K., The characterization of novel secreted Ly-6 proteins from rat urine by the combined use of two-dimensional gel electrophoresis, microbore high performance liquid chromatography and expressed sequence tag data. Proteomics, 2, 187-196 (2002).
  • 7) Görg, A., Postel, W., Weser, J., Gunther, S., Strahler, S. R., Hanash, S. M., and Somerlot, L., Elimination of point streaking on silver stained two-dimensional gels by addition of iodoacetamide to the equilibration buffer. Electrophoresis, 8, 122-124 (1987).
  • 8) Blum, H., Beier, H., and Gross, H. J., Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis, 8, 93-99 (1987).
  • 9) Anderson, N. G., and Anderson, L., The Human Protein Index. Clin. Chem., Apr. 28 (4 Pt 2), 739-748 (1982).
  • 10) Georgiou, H. M., Gregory, E. R., and Baker, M. S., Proteomic analysis of human plasma: Failure of centrifugal ultrafiltration to remove albumin and other high molecular weight proteins. Proteomics, 1, 1503-1506 (2001).

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.