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Bioscience, Biotechnology, and Biochemistry Volume 67, 2003 - Issue 9
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Original Articles

A Study of Tryptophan Fluorescence Quenching of Bifunctional Alginate Lyase from a Marine Bacterium Pseudoalteromonas sp. Strain No. 272 by Acrylamide

Yoshiko IWAMOTODivision of Biochemistry, Faculty of Fisheries, Nagasaki UniversityNagasaki 852-8521, Japan
,
Hidetoshi HIDAKADivision of Biochemistry, Faculty of Fisheries, Nagasaki UniversityNagasaki 852-8521, Japan
,
Tatsuya ODADivision of Biochemistry, Faculty of Fisheries, Nagasaki UniversityNagasaki 852-8521, Japan
&
Tsuyoshi MURAMATSUDivision of Biochemistry, Faculty of Fisheries, Nagasaki UniversityNagasaki 852-8521, Japan
Pages 1990-1992 | Received 14 Jan 2003, Accepted 23 May 2003, Published online: 22 May 2014

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  • 3) Iwamoto, Y., Iriyama, K., Osatomi, K., Oda, T., and Muramatsu, T., Primary structure and chemical modification of some amino acid residues of bifunctional alginate lyase from a marine bacterium Pseudoalteromonas sp. strain No. 272. J. Prot. Chem., 21, 453-461 (2002).
  • 4) Sawabe, T., Takahashi, H., Ezura, Y., and Gacesa, P., Cloning, sequence analysis and expression of Pseudoalteromonas elyakovii IAM 14594 gene (alyPEEC) encoding the extracellular alginate lyase. Carbohydr. Res., 335, 11-21 (2001).
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  • 11) Wong, T. Y., Preston, L. A., and Schiller, N. L., Alginate lyase: review of major sources and enzyme characteristics, structure-function analysis, biological role, and applications. Ann. Rev. Microbiol., 54, 289-340 (2000).
  • 12) Yoon, H. J., Hashimoto, W., Miyake, O., Murata, K., and Mikami, B., Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 Å resolution. J. Mol. Biol., 307, 9-16 (2001).
  • 2) Iwamoto, Y., Araki, R., Iriyama, K., Oda, T., Fukuda, H., Hayashida, S., and Muramatsu, T., Purification and characterization of bifunctional alginate lyase from Alteromonas sp. strain No. 272 and its action on saturated oligomeric substrates. Biosci. Biotechnol. Biochem., 65, 133-142 (2001).

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