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Original Articles

A Transient RNA Interference Assay System Using Arabidopsis Protoplasts

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Pages 2674-2677 | Received 25 Jul 2003, Accepted 15 Oct 2003, Published online: 22 May 2014

  • 1) Hannon, G. J., RNA interference. Nature, 418, 244-251 (2002).
  • 2) Denli, A. M., and Hannon, G. J., RNAi: an ever-growing puzzle. Trends Biochem. Sci., 28, 196-201 (2003).
  • 3) Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., and Mello, C. C., Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature, 391, 806-811 (1998).
  • 4) Wang, M. B., and Waterhouse, P. M., Application of gene silencing in plants. Curr. Opin. Plant Biol., 5, 146-150 (2002).
  • 5) Schweizer, P., Pokorny, J., Schulze-Lefert, P., and Dudler, R., Technical advance. Double-stranded RNA interferes with gene function at the single-cell level in cereals. Plant J., 24, 895-903 (2000).
  • 6) Akashi, H., Miyagishi, M., and Taira, K., Suppression of gene expression by RNA interference in cultured plant cells. Antisense Nucleic Acid Drug Dev., 11, 359-367 (2001).
  • 7) Johansen, L. K., and Carrington, J. C., Silencing on the spot. Induction and suppression of RNA silencing in the Agrobacterium-mediated transient expression system. Plant Physiol., 126, 930-938 (2001).
  • 8) Ruiz, M. T., Voinnet, O., and Baulcombe, D. C., Initiation and maintenance of virus-induced gene silencing. Plant Cell, 10, 937-946 (1998).
  • 9) Ratcliff, F., Martin-Hernandez, A. M., and Baulcombe, D. C., Technical advance. Tobacco rattle virus as a vector for analysis of gene function by silencing. Plant J., 25, 237-245 (2001).
  • 10) Gossele, V., Fache, I., Meulewaeter, F., Cornelissen, M., and Metzlaff, M., SVISS—a novel transient gene silencing system for gene function discovery and validation in tobacco plants. Plant J., 32, 859-866 (2002).
  • 11) Klahre, U., Crete, P., Leuenberger, S. A., Iglesias, V. A., and Meins, F., Jr., High molecular weight RNAs and small interfering RNAs induce systemic posttranscriptional gene silencing in plants. Proc. Natl. Acad. Sci. USA, 99, 11981-11986 (2002).
  • 12) Guo, H. S., Fei, J. F., Xie, Q., and Chua, N. H., A chemical-regulated inducible RNAi system in plants. Plant J., 34, 383-392 (2003).
  • 13) Sheen, J., A transient expression assay using Arabidopsis mesophyll protoplasts. http://genetics. mgh.harvard.edu/sheenweb/(2002).
  • 14) Axelos, M., Curie, C., Mazzolini, L., Bardet, C., and Lescure, B., A protocol for transient gene expression in Arabidopsis thaliana protoplasts isolated from cell suspension cultures. Plant Physiol. Biochem., 30, 123-128 (1992).
  • 15) Kang, B. H., Busse, J. S., Dickey, C., Rancour, D. M., and Bednarek, S. Y., The Arabidopsis cell plate-associated dynamin-like protein, ADL1Ap, is required for multiple stages of plant growth and development. Plant Physiol., 126, 47-68 (2001).
  • 16) Miyagishi, M., and Taira, K., U6 promoter driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells. Nat. Biotechnol., 20, 497-500 (2002).
  • 17) McManus, M. T., and Sharp, P. A., Gene silencing in mammals by small interfering RNAs. Nat. Rev. Genet., 3, 737-747 (2002).
  • 18) Cerutti, H., RNA interference: traveling in the cell and gaining functions? Trends Genet., 19, 39-46 (2003).

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