- 1) Schlumbaum, A., Mauch, F., Vogeli, U., and Boller, T., Plant chitinases are potent inhibitors of fungal growth. Nature, 324, 365–367 (1986).
- 2) Broekaert, W. F., Van Pariji, J., Allen, A. K., and Peumans, W. J., Comparison of some molecular, enzymatic and antifungal properties of chitinases from thorn-apple, tobacco and wheat. Physiol. Mol. Plant Pathol., 33, 319–331 (1988).
- 3) Broglie, K., Chet, I., Holliday, M., Cressman, R., Biddle, P., Knowlton, S., Mauvais, C. J., and Broglie, R., Transgenic plants with enhanced resistance to the fungal pathogen Rizoctonia solani. Science, 254, 1194–1197 (1991).
- 4) Sela-Buurlage, M. B., Ponstein, A. S., Bres-Vloemans, S. A., Melchers, L. S., van den Elzen, P. J. M., and Cornelissen, B. J. C., Only specific tobacco (Nicotiana tabacum) chitinases and β-1,3-glucanases exhibit antifungal activity. Plant Physiol., 101, 857–863 (1993).
- 5) Henrissat, B., and Bairoch, A., New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J., 293, 781–788 (1993).
- 6) Yamagami, T., and Funatsu, G., Limited proteolysis and reduction-carboxymethylation of rye seed chitinase-a: role of the chitin-binding domain in its chitinase action. Biosci. Biotechnol. Biochem., 60, 1081–1086 (1996).
- 7) Iseli, B., Boller, T., and Neuhaus, J. M., The N-terminal cysteine-rich domain of tabacco class I chitinase is essential for chitin binding but not for catalytic or antifungal activity. Plant Physiol., 103, 221–226 (1993).
- 8) Yamagami, T., and Funatsu, G., Purification and some properties of three chitinases from the seeds of rye (Secale cereale). Biosci. Biotechnol. Biochem., 57, 643–647 (1993).
- 9) Yamagami, T., and Funatsu, G., The complete amino acid sequence of chitinase-c from the seeds of rye (Secale cereale). Biosci. Biotechnol. Biochem., 57, 1854–1861 (1993).
- 10) Yamagami, T., and Funatsu, G., The complete amino acid sequence of chitinase-a from the seeds of rye (Secale cereale). Biosci. Biotechnol. Biochem., 58, 322–329 (1994).
- 11) Taira, T., Yamagami, T., Aso, Y., Ishiguro, M., and Ishihara, M., Localization, accumulation, and antifungal activity of chitinases in rye (Secale cereale) seed. Biosci. Biotechnol. Biochem., 65, 2710–2718 (2001).
- 12) Taira, T., Ohnuma, T., Yamagami, T., Aso, Y., Ishiguro, M., and Ishihara, M., Antifungal activity of rye (Secale cereale) seed chitinases: the different binding manner of class I and class II chitinases to the fungal cell walls. Biosci. Biotechnol. Biochem., 66, 970–977 (2002).
- 13) Ohnuma, T., Yagi, M., Yamagami, T., Taira, T., Aso, Y., and Ishiguro, M., Molecular cloning, functional expression, and mutagenesis of cDNA encoding rye (Secale cereale) seed chitinase-c. Biosci. Biotechnol. Biochem., 66, 277–284 (2002).
- 14) Chen, Z., Simple modifications to increase specificity of the 5′ RACE procedure. Trends in Genetics, 12, 87–88 (1996).
- 15) Sanger, F., Nickelsen, S., and Coulson, A. R., DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA, 74, 5463–5467 (1977).
- 16) Studier, F. W., Rosenberg, A. H., Dunn, J. J., and Dubendorff, J. W., Use of T7 RNA polymerase to direct expression of cloned gene. Methods Enzymol., 185, 60–89 (1990).
- 17) Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685 (1970).
- 18) Towbin, H., Staehelin, T., and Gordon, L., Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some application. Proc. Natl. Acad. Sci. USA, 76, 4350–4354 (1979).
- 19) Imoto, T., and Yagishita, K., A simple activity measurement of lysozyme. Agric. Biol. Chem., 35, 1154–1156 (1971).
- 20) Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., and Klenk, D. C., Measurement of protein using bicinchoninic acid. Anal. Biochem., 150, 76–85 (1985).
- 21) Von Heijine, G., Patterns of amino acids near signal sequence clevage sites. Eur. J. Biochem., 133, 17–21 (1983).
- 22) Yeh, S., Moffatt, B. A., Griffith, M., Xiong, F., Yang, D. S., Wiseman, S. B., Sarhan, F., Danyluk, J., Xue, Y. Q., Hew, C. L., Doherty-Kirby, A., and Lajoie, G., Chitinase genes responsive to cold encode antifreeze proteins in winter cereals. Plant Physiol., 124, 1251–1264 (2000).
- 23) Shinshi, H., Neuhaus, J. M., Ryals, J., and Meins, F. Jr., Structure of a tobacco endochitinase gene: evidence that different chitinase genes can arise by transposition of sequences encoding a cysteine-rich domain. Plant Mol. Biol., 14, 357–368 (1990).
- 24) Yamagami, T., and Funatsu, G., Involvements Trp23 in the chitin-binding and of Trp131 in the chitinase activity of rye seed chitinase-a. Biosci. Biotechnol. Biochem., 61, 1819–1825 (1997).
- 25) Leah, R., Tommerup, H., Svendsen, I., and Mundy, J., Biochemical and molecular characterization of three barley seed proteins with antifungal properties. J. Biol. Chem., 266, 1564–1573 (1990).
- 26) Iseli, B., Boller, T., and Neuhaus, J. M., The N-terminal cysteine-rich domain of tobacco class I chitinase is essential for chitin binding but not for catalytic or antifungal activity. Plant Physiol., 103, 221–226 (1993).
- 27) Yamagami, T., and Funatsu, G., Limited proteolysis and reduction-carboxymethylation of rye seed chitinase-a: role of the chitin-binding domain in its chitinase action. Biosci. Biotechnol. Biochem., 60, 1081–1086 (1996).
- 28) Neuhaus, J. M., Sticher, L., Meins, F. Jr., and Boller, T., A short C-terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole. Proc. Natl. Acad. Sci. USA, 88, 10362–10366 (1991).
- 29) Bessette, P. H., Aslund, F., Beckwith, J., and Georgiou, G., Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm. Proc. Natl. Acad. Sci. USA, 96, 13703–13708 (1999).
- 30) Uchiyama, T., Katouno, F., Nikaidou, N., Nonala, T., Sugiyama, J., and Watanabe, T., Roles of the exposed aromatic residues in crystalline chitin hydrolysis by chitinase A from Serratia marcescens 2170. J. Biol. Chem., 276, 41343–41349 (2001).
Full access
Molecular Cloning, Functional Expression, and Mutagenesis of cDNA Encoding Class I Chitinase from Rye (Secale cereale) Seeds
Reprints and Corporate Permissions
Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?
To request a reprint or corporate permissions for this article, please click on the relevant link below:
Academic Permissions
Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?
Obtain permissions instantly via Rightslink by clicking on the button below:
If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.
Related research
People also read lists articles that other readers of this article have read.
Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.
Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.