237
Views
16
CrossRef citations to date
0
Altmetric
Original Articles

Hydrolysis of Micellar Phosphatidylcholine Accelerates Cholesterol Absorption in Rats and Caco-2 Cells

, , , , , , & show all
Pages 1726-1732 | Received 11 Apr 2005, Accepted 26 May 2005, Published online: 22 May 2014

  • 1) Hui, D. Y., and Howles, P. N., Carboxyl ester lipase: structure-function relationship and physiological role in lipoprotein metabolism and atherosclerosis. J. Lipid Res., 43, 2017–2030 (2002).
  • 2) Bhat, S. G., and Brockman, H. L., The role of cholesteryl ester hydrolysis and synthesis in cholesterol transport across rat intestinal mucosal membrane. A new concept. Biochem. Biophys. Res. Commun., 109, 486–492 (1982).
  • 3) Gallo, L. L., Clark, S. B., Myers, S., and Vahouny, G. V., Cholesterol absorption in rat intestine: role of cholesterol esterase and acyl coenzyme A: cholesterol acyltransferase. J. Lipid Res., 25, 604–612 (1984).
  • 4) Lopez-Candales, A., Bosner, M. S., Spilburg, C. A., and Lange, L. G., Cholesterol transport function of pancreatic cholesterol esterase: directed sterol uptake and esterification in enterocytes. Biochemistry, 32, 12085–12089 (1993).
  • 5) Gallo, L. L., Newbill, T., Hyun, J., Vahouny, G. V., and Treadwell, C. R., Role of pancreatic cholesterol esterase in the uptake and esterification of cholesterol by isolated intestinal cells (39921). Proc. Soc. Exp. Biol. Med., 156, 277–281 (1977).
  • 6) Huang, Y., and Hui, D. Y., Metabolic fate of pancreas-derived cholesterol esterase in intestine: an in vitro study using Caco-2 cells. J. Lipid Res., 31, 2029–2037 (1990).
  • 7) Shamir, R., Johnson, W. J., Zolfaghari, R., Lee, H. S., and Fisher, E. A., Role of bile salt-dependent cholesteryl ester hydrolase in the uptake of micellar cholesterol by intestinal cells. Biochemistry, 34, 6351–6358 (1995).
  • 8) Howles, P. N., Carter, C. P., and Hui, D. Y., Dietary free and esterified cholesterol absorption in cholesterol esterase (bile salt-stimulated lipase) gene-targeted mice. J. Biol. Chem., 271, 7196–7202 (1996).
  • 9) Ikeda, I., Matsuoka, R., Hamada, T., Mitsui, K., Imabayashi, S., Uchino, A., Sato, M., Kuwano, E., Itamura, T., Yamada, K., Tanaka, K., and Imaizumi, K., Cholesterol esterase accelerates intestinal cholesterol absorption. Biochim. Biophys. Acta, 1571, 34–44 (2002).
  • 10) Carey, M. C., Small, D. M., and Bliss, C. M., Lipid digestion and absorption. Annu. Rev. Physiol., 45, 651–677 (1983).
  • 11) Ranheim, T., Gedde-Dahl, A., Rustan, A. C., and Drevon, C. A., Influence of eicosapentaenoic acid (20:5, n-3) on secretion of lipoproteins in Caco-2 cells. J. Lipid Res., 33, 1281–1293 (1992).
  • 12) Folch, J., Lees, M., and Sloane-Stanley, G. H., A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem., 226, 497–509 (1957).
  • 13) Rouser, G., Siakotos, A. N., and Fleischer, S., Quantitative analysis of phospholipids by thin-layer chromatography and phosphorous analysis of spots. Lipids, 1, 85–86 (1966).
  • 14) Ikeda, I., Tanaka, K., Sugano, M., Vahouny, G. V., and Gallo, L. L., Discrimination between cholesterol and sitosterol for absorption in rats. J. Lipid Res., 29, 1583–1591 (1988).
  • 15) Greten, H., Raetzer, H., Stiehl, A., and Schettler, G., The effect of polyunsaturated phosphatidylcholine on plasma lipids and fecal sterol excretion. Atherosclerosis, 36, 81–88 (1980).
  • 16) O’Mullane, J. E., and Hawthorne, J. N., A comparison of the effects of feeding linoleic acid-rich lecithin or corn oil on cholesterol absorption and metabolism in the rat. Atherosclerosis, 45, 81–90 (1982).
  • 17) Hollander, D., and Morgan, D., Effect of plant sterols, fatty acids and lecithin on cholesterol absorption in vivo in the rat. Lipids, 15, 395–400 (1980).
  • 18) Kesaniemi, Y. A., and Grundy, S. M., Effects of dietary polyenylphosphatidylcholine metabolism of cholesterol and triglycerides in hypertriglyceridemic patients. Am. J. Clin. Nutr., 43, 98–107 (1986).
  • 19) Richmond, B. L., Boileau, A. C., Zheng, S., Huggins, K. W., Granholm, N. A., Tso, P., and Hui, D. Y., Compensatory phospholipid digestion is required for cholesterol absorption in pancreatic phospholipase A2-deficient mice. Gastroenterology, 120, 1193–1202 (2001).
  • 20) Mackay, K., Starr, J. R., Lawn, R. M., and Ellsworth, J. L., Phosphatidylcholine hydrolysis is required for pancreatic cholesterol esterase- and phospholipase A2-facilitated cholesterol uptake into intestinal Caco-2 cells. J. Biol. Chem., 272, 13380–13389 (1997).
  • 21) Homan, R., and Hamelehle, K. L., Phospholipase A2 relieves phosphatidylcholine inhibition of micellar cholesterol absorption and transport by human intestinal cell line Caco-2. J. Lipid Res., 39, 1197–1209 (1998).
  • 22) Rampone, A. J., and Machida, C. M., Mode of action of lecithin in suppressing cholesterol absorption. J. Lipid Res., 22, 744–752 (1981).
  • 23) Gallo, L. L., Chiang, Y., Vahouny, G. V., and Treadwell, C. R., Localization and origin of rat intestinal cholesterol esterase determined by immunocytochemistry. J. Lipid Res., 21, 537–545 (1980).
  • 24) Field, F. J., Albright, E., and Mathur, S. N., Regulation of cholesterol esterification by micellar cholesterol in CaCo-2 cells. J. Lipid Res., 28, 1057–1066 (1987).

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.