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Bioscience, Biotechnology, and Biochemistry Volume 69, 2005 - Issue 9
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Original Articles

Analysis of Chitin at the Hyphal Tip of Candida albicans Using Calcofluor White

Hiroshi WATANABEDepartment of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University
,
Masayuki AZUMADepartment of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University
,
Koichi IGARASHIDepartment of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University
&
Hiroshi OOSHIMADepartment of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University
Pages 1798-1801 | Received 08 Apr 2005, Accepted 27 May 2005, Published online: 22 May 2014

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  • 2) Shepherd, M. G., Yin, C. Y., Ram, S. P., and Sullivan, P. A., Germ tube induction in Candida albicans. Can. J. Microbiol., 26, 21–26 (1980).
  • 3) Lee, K. L., Buckley, H. R., and Campbell, C. C., An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida albicans. Sabouraudia, 13, 148–153 (1975).
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  • 5) Sudoh, M., Tatsuno, K., Ono, N., Ohta, A., Chibana, H., Yamada-Okabe, H., and Arisawa, M., The Candida albicans CHS4 gene complements a Saccharomyces cerevisiae skt5/chs4 mutation and is involved in chitin biosynthesis. Microbiology, 145, 1613–1622 (1999).
  • 6) Bulawa, C. E., Slater, M., Cabib, E., Au-Young, J., Sburlati, A., Adair, W. L., Jr., and Robbins, P. W., The S. cerevisiae structural gene for chitin synthase is not required for chitin synthesis in vivo. Cell, 46, 213–225 (1986).
  • 7) Elson, L. A., and Morgan, W., A colorimetric method for the determination of glucosamine and chondrosamine. Biochem. J., 27, 1824–1828 (1933).
  • 8) Kapteyn, J. C., Van Den Ende, H., and Klis, F. M., The contribution of cell wall proteins to the organization of the yeast cell wall. Biochim. Biophys. Acta, 1426, 373–383 (1999).
  • 9) Popolo, L., and Val, M., The Gas1 glycoprotein, a putative wall polymer cross-linker. Biochim. Biophys. Acta, 1426, 385–400 (1999).

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