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Bioscience, Biotechnology, and Biochemistry Volume 69, 2005 - Issue 12
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Original Articles

Development of a Modified Positive Selection Medium That Allows to Isolate Aspergillus oryzae Strains Cured of the Integrated niaD-Based Plasmid

Kazutomo ISHIDepartment of Biotechnology, The University of Tokyo
,
Taisuke WATANABEDepartment of Biotechnology, The University of Tokyo
,
Praveen Rao JUVVADIDepartment of Biotechnology, The University of Tokyo
,
Jun-ichi MARUYAMADepartment of Biotechnology, The University of Tokyo
&
Katsuhiko KITAMOTODepartment of Biotechnology, The University of Tokyo
Pages 2463-2465 | Received 28 Jul 2005, Accepted 26 Aug 2005, Published online: 22 May 2014

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  • 2) Shortle, D., Novick, P., and Botstein, D., Construction and genetic characterization of temperature-sensitive mutant alleles of the yeast actin gene. Proc. Natl. Acad. Sci. U.S.A., 81, 4889–4893 (1984).
  • 3) Dunne, P. W., and Oakley, B. R., Mitotic gene conversion, reciprocal recombination and gene replacement at the benA, beta-tubulin, locus of Aspergillus nidulans. Mol. Gen. Genet., 213, 339–345 (1988).
  • 4) Ward, M., Wilson, L. J., and Kodama, K. H., Use of Aspergillus overproducing mutants, cured for integrated plasmid, to overproduce heterologous proteins. Appl. Microbiol. Biotechnol., 39, 738–743 (1993).
  • 5) Minetoki, T., Nunokawa, Y., Gomi, K., Kitamoto, K., Kumagai, C., and Tamura, G., Deletion analysis of promoter elements of the Aspergillus oryzae agdA gene encoding α-glucosidase. Curr. Genet., 30, 432–438 (1996).
  • 6) Unkles, S. E., Campbell, E. I., Ruiter-Jacobs, Y. M. J. T., Broekhuijsen, M., Macro, J. A., Carrez, D., Contreras, R., van den Hondel, C. A. M. J. J., and Kinghorn, J. R., The development of a homologous transformation system for Aspergillus oryzae based on the nitrate assimilation pathway: a convenient and general selection system for filamentous fungal transformation. Mol. Gen. Genet., 218, 99–104 (1989).
  • 7) Maruyama, J., Nakajima, H., and Kitamoto, K., Visualization of nuclei in Aspergillus oryzae with EGFP and analysis of the number of nuclei in each conidium by FACS. Biosci. Biotechnol. Biochem., 65, 1504–1510 (2001).
  • 8) Muro-Pastor, M. I., Gonzalez, R., Strauss, J., Narendja, F., and Scazzocchio, C., The GATA factor AreA is essential for chromatin remodelling in a eukaryotic bidirectional promoter. EMBO J., 15, 1584–1597 (1999).
  • 9) Morozov, I. Y., Galbis-Martinez, M., Jones, M. G., and Caddick, M. X., Characterization of nitrogen metabolite signaling in Aspergillus via the regulated degradation of areA mRNA. Mol. Microbiol., 42, 269–277 (2001).

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