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Bioscience, Biotechnology, and Biochemistry Volume 69, 2005 - Issue 1
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Original Articles

The Class V Chitin Synthase Gene csmA Is Crucial for the Growth of the chsA chsC Double Mutant in Aspergillus nidulans

Emi YAMADADepartment of Biotechnology, The University of Tokyo
,
Masayuki ICHINOMIYADepartment of Biotechnology, The University of Tokyo
,
Akinori OHTADepartment of Biotechnology, The University of Tokyo
&
Hiroyuki HORIUCHIDepartment of Biotechnology, The University of Tokyo
Pages 87-97 | Received 12 Aug 2004, Accepted 20 Oct 2004, Published online: 22 May 2014

  • 1) Yanai, K., Kojima, N., Takaya, N., Horiuchi, H., Ohta, A., and Takagi, M., Isolation and characterization of two chitin synthase genes from Aspergillus nidulans. Biosci. Biotechnol. Biochem., 58, 1828–1835 (1994).
  • 2) Motoyama, T., Kojima, N., Horiuchi, H., Ohta, A., and Takagi, M., Isolation of a chitin synthase gene (chsC) of Aspergillus nidulans. Biosci. Biotechnol. Biochem., 58, 2254–2257 (1994).
  • 3) Motoyama, T., Fujiwara, M., Kojima, N., Horiuchi, H., Ohta, A., and Takagi, M., The Aspergillus nidulans genes chsA and chsD encode chitin synthases which have redundant functions in conidia formation. Mol. Gen. Genet., 251, 442–450 (corrigendum in Mol. Gen. Genet., 253, 520–528) (1996).
  • 4) Fujiwara, M., Horiuchi, H., Ohta, A., and Takagi, M., A novel fungal gene encoding chitin synthase with a myosin motor-like domain. Biochem. Biophys. Res. Commun., 236, 75–78 (1997).
  • 5) Beth-Din, A., Specht, C. A., Robbins, P. W., and Yarden, O., chs-4, a class IV chitin synthase gene from Neurospora crassa. Mol. Gen. Genet., 250, 214–222 (1996).
  • 6) Specht, C. A., Liu, Y., Robbins, P. W., Bulawa, C. E., Iartchouk, N., Winter, K. R., Riggle, P. J., Rhodes, J. C., Dodge, C. L., Culp, D. W., and Borgia, P. T., The chsD and chsE genes of Aspergillus nidulans and their roles in chitin synthesis. Fungal Genet. Biol., 20, 153–167 (1996).
  • 7) Roncero, C., The genetic complexity of chitin synthesis in fungi. Curr. Genet., 41, 367–378 (2002).
  • 8) Borgia, P. T., Iartchouk, N., Riggle, P. J., Winter, K. R., Koltin, Y., and Bulawa, C. E., The chsB gene of Aspergillus nidulans is necessary for normal hyphal growth and development. Fungal Genet. Biol., 20, 193–203 (1996).
  • 9) Horiuchi, H., Fujiwara, M., Yamashita, S., Ohta, A., and Takagi, M., Proliferation of intrahyphal hyphae caused by disruption of csmA, which encodes a class V chitin synthase with a myosin motor-like domain in Aspergillus nidulans. J. Bacteriol., 181, 3721–3729 (1999).
  • 10) Ichinomiya, M., Horiuchi, H., and Ohta, A., Different functions of the class I and class II chitin synthase genes, chsC and chsA, are revealed by repression of chsB expression in Aspergillus nidulans. Curr. Genet., 42, 51–58 (2002).
  • 11) Fujiwara, M., Ichinomiya, M., Motoyama, T., Horiuchi, H., Ohta, A., and Takagi, M., Evidence that the Aspergillus nidulans class I and class II chitin synthase genes, chsC and chsA, share critical roles in hyphal wall integrity and conidiophore development. J. Biochem. (Tokyo), 127, 359–366 (2000).
  • 12) Kapteyn, J. C., Ram, A. F., Groos, E. M., Kollar, R., Montijn, R. C., Van Den Ende, H., Llobell, A., Cabib, E., and Klis, F. M., Altered extent of cross-linking of β1,6-glucosylated mannoproteins to chitin in Saccharomyces cerevisiae mutants with reduced cell wall β1,3-glucan content. J. Bacteriol., 179, 6279–6284 (1997).
  • 13) Ram, A. F., Kapteyn, J. C., Montijn, R. C., Caro, L. H., Douwes, J. E., Baginsky, W., Mazur, P., Van Den Ende, H., and Klis, F. M., Loss of the plasma membrane-bound protein Gas1p in Saccharomyces cerevisiae results in the release of β1,3-glucan into the medium and induces a compensation mechanism to ensure cell wall integrity. J. Bacteriol., 180, 1418–1424 (1998).
  • 14) Valdivieso, M. H., Ferrario, L., Vai, M., Duran, A., and Popolo, L., Chitin synthesis in a gas1 mutant of Saccharomyces cerevisiae. J. Bacteriol., 182, 4752–4757 (2000).
  • 15) Heinisch, J. J., Lorberg, A., Schmitz, H. P., and Jacoby, J. J., The protein kinase C-mediated MAP kinase pathway involved in the maintenance of cellular integrity in Saccharomyces cerevisiae. Mol. Microbiol., 32, 671–680 (1999).
  • 16) Ichinomiya, M., Motoyama, T., Fujiwara, M., Takagi, M., Horiuchi, H., and Ohta, A., Repression of chsB expression reveals the functional importance of class IV chitin synthase gene chsD in hyphal growth and conidiation of Aspergillus nidulans. Microbiology, 148, 1335–1347 (2002).
  • 17) Takeshita, N., Ohta, A., and Horiuchi, H., csmA, a gene encoding a class V chitin synthase with a myosin motor-like domain of Aspergillus nidulans, is translated as a single polypeptide and regulated in response to osmotic conditions. Biochem. Biophys. Res. Commun., 298, 103–109 (2002).
  • 18) Buxton, F. P., Gwynne, D. I., and Davies, R. W., Cloning of a new bidirectionally selectable marker for Aspergillus strains. Gene, 84, 329–334 (1989).
  • 19) Rowlands, R. T., and Turner, G., Nuclear and extranuclear inheritance of oligomycin resistance in Aspergillus nidulans. Mol. Gen. Genet., 126, 201–216 (1973).
  • 20) May, G. S., Mcgoldrick, C. A., Holt, C. L., and Denison, S. H., The bimB3 mutation of Aspergillus nidulans uncouples DNA replication from the completion of mitosis. J. Biol. Chem., 267, 15737–15743 (1992).
  • 21) Sambrook, J., Fritsch, E. F., and Maniatis, T., Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
  • 22) Oakley, C. E., Weil, C. F., Kretz, P. L., and Oakley, B. R., Cloning of the riboB locus of Aspergillus nidulans. Gene, 53, 293–298 (1987).
  • 23) Jung, U.-S., and Levin, D., Genome-wide analysis of gene expression regulated by the yeast cell wall integrity signalling pathway. Mol. Microbiol., 34, 1049–1057 (1999).
  • 24) Valdivia, R. H., and Schekman, R., The yeasts Rho1p and Pkc1p regulate the transport of chitin synthase III (Chs3p) from internal stores to the plasma membrane. Proc. Natl. Acad. Sci. U.S.A., 100, 10287–10292 (2003).
  • 25) Culp, D. W., Dodge, C. L., Miao, Y., Li, L., Sag-Ozkal, D., and Borgia, P. T., The chsA gene from Aspergillus nidulans is necessary for maximal conidiation. FEMS Microbiol. Lett., 182, 349–353 (2000).
  • 26) Liu, H., Kauffman, S., Becker, J. M., and Szaniszlo, P. J., Wangiella (Exophiala) dermatitidis WdChs5p, a class V chitin synthase, is essential for sustained cell growth at temperature of infection. Eukaryot. Cell, 3, 40–51 (2004).
  • 27) Yamada, O., Lee, B. R., and Gomi, K., Transformation system for Aspergillus oryzae with double auxotrophic mutations, niaD and sC. Biosci. Biotechnol. Biochem., 61, 1367–1369 (1997).

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