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Bioscience, Biotechnology, and Biochemistry Volume 70, 2006 - Issue 1
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Original Articles

Atomic Force Microscopic Observation of Escherichia coli Ribosomes in Solution

Toshihiko MATSUURAInstitute of Scientific and Industrial Research, Osaka University;Technology Research Laboratory, Shimadzu Corporation
,
Hiroyuki TANAKAInstitute of Scientific and Industrial Research, Osaka University
,
Takuya MATSUMOTOInstitute of Scientific and Industrial Research, Osaka University
&
Tomoji KAWAIInstitute of Scientific and Industrial Research, Osaka University
Pages 300-302 | Received 29 Aug 2005, Accepted 20 Oct 2005, Published online: 22 May 2014

  • 1) Nierhaus, K. H., and Wilson, D. N., “Protein Synthesis and Ribosome Structure”, Wiley-VCH, Weinheim, pp. 1–51 (2004).
  • 2) Wimberly, B. T., Brodersen, D. E., Clemons, W. M., Morgan-Warren, R. J., Carter, A. P., Vonrhein, C., Hartsch, T., and Ramakrishnan, V., Structure of the 30S ribosomal subunit. Nature, 407, 327–339 (2000).
  • 3) Ban, N., Nissen, P., Hansen, J., Moore, P. B., and Steitz, T. A., The complete atomic structure of the large ribosomal subunit at 2.4 angstrom resolution. Science, 289, 905–920 (2000).
  • 4) Yusupov, M. M., Yusupova, G. Z., Baucom, A., Lieberman, K., Earnest, T. N., Cate, J. H. D., and Noller, H. F., Crystal structure of the ribosome at 5.5 angstrom resolution. Science, 292, 883–896 (2001).
  • 5) Ramakrishnan, V., Ribosome structure and the mechanism of translation. Cell, 108, 557–572 (2002).
  • 6) Frank, J., Cryo-electron microscopy as an investigative tool: the ribosome as an example. BioEssays, 23, 725–732 (2001).
  • 7) Gao, H., Sengupta, J., Valle, M., Korostelev, A., Eswar, N., Stagg, S. M., Roey, P. V., Agrawal, R. K., Harvey, S. C., Sali, A., Chapman, M. S., and Frank, J., Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement. Cell, 113, 789–801 (2003).
  • 8) Gilbert, R. J. C., Fucini, P., Connell, S., Fuller, S. D., Nierhaus, K. H., Robinson, C. V., Dobson, C. M., and Stuart, D. I., Three-dimensional structures of translating ribosomes by cryo-EM. Mol. Cell, 14, 57–66 (2004).
  • 9) Thomson, N. H., Smith, B. L., Almqvist, N., Schmitt, L., Kashlev, M., Koo, E. T., and Hansma, P. K., Oriented, active Escherichia coli RNA polymerase: an atomic force microscope study. Biophys. J., 76, 1024–1033 (1999).
  • 10) Ikai, A., STM and AFM of bio/organic molecules and structures. Surf. Sci. Rep., 26, 261–332 (1996).
  • 11) Hansma, H. G., Varieties of imaging with scanning probe microscopes. Proc. Natl. Acad. Sci. U.S.A., 96, 14678–14680 (1999).
  • 12) Jamdt, K. D., Atomic force microscopy of biomaterials surfaces and interfaces. Surf. Sci., 491, 303–332 (2001).
  • 13) Li, M.-Q., Xu, L., and Ikai, A., Atomic force microscope imaging of ribosome and chromosome. J. Vac. Sci. Technol. B, 14, 1410–1412 (1996).
  • 14) Wu, X.-H., Liu, W.-Y., Xu, L., and Li, M.-Q., Topography of ribosomes and initiation complexes from rat liver as revealed by atomic force microscopy. Biol. Chem., 378, 363–372 (1997).
  • 15) Yoshida, T., Wakiyama, M., Yazaki, K., and Miura, K., Transmission electron and atomic force microscopic observation of polysomes on carbon-coated grids prepared by surface spreading. J. Electron. Microsc., 46, 503–506 (1997).
  • 16) Fritzsche, W., and Henderson, E., Ribosome substructure investigated by scanning force microscopy and image processing. J. Microsc. (Oxford), 189, 50–56 (1998).
  • 17) Vanzi, F., Vladimirov, S., Knudsen, C. R., Goldman, Y. E., and Cooperman, B. S., Protein synthesis by single ribosomes. RNA, 9, 1174–1179 (2003).
  • 18) Sawata, S. Y., Suyama, E., and Taira, K., A system based on specific protein–RNA interactions for analysis of target protein–protein interactions in vitro: successful selection of membrane-bound Bak-Bcl-xL proteins in vitro. Protein Engineering, Design. Selection, 17, 501–508 (2004).
  • 19) Matsuura, T., Kobayashi, K., Tanaka, H., Matsumoto, T., and Kawai, T., Scanning force microscopic studies of Escherichia coli ribosomes on solid substrate surface. Jpn. J. Appl. Phys., 43, 4599–4601 (2004).
  • 20) Spedding, G., “Ribosomes and Protein Synthesis: A Practical Approach”, IRL press at Oxford University Press, New York, pp. 1–29 (1990).
  • 21) Sytnik, A., Vladimirov, S., Jia, Y., Li, L., Cooperman, B. S., and Hochstrasser, R. M., Peptidyl transferase center activity observed in single ribosomes. J. Mol. Biol., 285, 49–54 (1999).
  • 22) Hansma, H. G., Vesenka, J., Siegerist, C., Kelderman, G., Morrett, H., Sinsheimer, R. L., Elings, V., Bustamante, C., and Hansma, P. K., Reproducible imaging and dissection of plasmid DNA under liquid with the atomic force microscope. Science, 256, 1180–1184 (1992).
  • 23) Pietrement, O., Pastre, D., Fusil, S., Jeusset, J., David, M., Landousy, F., Hamon, L., Zozime, A., and Cam, E. L., Reversible binding of DNA on NiCl2-treated mica by varying the ionic strength. Langmuir, 19, 2536–2539 (2003).
  • 24) Riener, C. K., Stroh, C. M., Ebner, A., Klampfl, C., Gall, A. A., Romanin, C., Lyubchenko, Y. L., Hinterdorfer, P., and Gruber, H. J., Simple test system for single molecule recognition force microscopy. Anal. Chim. Acta, 479, 59–75 (2003).
  • 25) Hansma, H. G., Polysaccharide helices in the atomic force microscope. Biophys. J., 68, 3–4 (1995).
  • 26) Sekiguchi, H., Arakawa, H., Taguchi, H., Ito, T., Kokawa, R., and Ikai, A., Specific interaction between GroEL and denatured protein measured by compression-free force spectroscopy. Biophys. J., 85, 484–490 (2003).
  • 27) Arnstein, H. R. V., and Cox, R. A., “Protein Biosynthesis”, IRL Press at Oxford University Press, New York, pp. 39–60 (1992).
  • 28) Moreno-Herrero, F., Colchero, J., and Baro, A. M., DNA height in scanning force microscopy. Ultramicroscopy, 96, 167–174 (2003).

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