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Original Articles

Relationship between the Stability of Hen Egg-White Lysozymes Mutated at Sites Designed to Interact with α-Helix Dipoles and Their Secretion Amounts in Yeast

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Pages 2952-2961 | Received 06 Jun 2007, Accepted 04 Sep 2007, Published online: 22 May 2014

  • 1) Wilson, K. P., Malcolm, B. A., and Matthews, B. W., Structural and thermodynamic analysis of compensating mutations within the core of chicken egg white lysozyme. J. Biol. Chem., 267, 10842–10849 (1992).
  • 2) Taniyama, Y., Ogasahara, K., Yutani, K., and Kikuchi, M., Folding mechanism of mutant human lysozyme C77/95A with increased secretion efficiency in yeast. J. Biol. Chem., 267, 4619–4624 (1992).
  • 3) Booth, D. R., Sunde, M., Bellotti, V., Robinson, C. V., Hutchinson, W. L., Fraser, P. E., Hawkins, P. N., Dobson, C. M., Radford, S. E., Blake, C. C., and Pepys, M. B., Instability, unfolding and aggregation of human lysozyme variants underlying amyloid fibrillogenesis. Nature, 385, 787–793 (1997).
  • 4) Shoemaker, K. R., Kim, P. S., York, E. J., Stewart, J. M., and Baldwin, R. L., Tests of the helix dipole model for stabilization of alpha-helices. Nature, 326, 563–567 (1987).
  • 5) Richardson, J. S., and Richardson, D. C., Amino acid preferences for specific locations at the ends of alpha helices. Science, 240, 1648–1652 (1988).
  • 6) Nicholson, H., Becktel, W. J., and Matthews, B. W., Enhanced protein thermostability from designed mutations that interact with alpha-helix dipoles. Nature, 336, 651–656 (1988).
  • 7) Nicholson, H., Anderson, D. E., Dao-pin, S., and Matthews, B. W., Analysis of the interaction between charged side chains and the alpha-helix dipole using designed thermostable mutants of phage T4 lysozyme. Biochemistry, 30, 9816–9828 (1991).
  • 8) Motoshima, H., Mine, S., Masumoto, K., Abe, Y., Iwashita, H., Hashimoto, Y., Chijiiwa, Y., Ueda, T., and Imoto, T., Analysis of the stabilization of hen lysozyme by helix macrodipole and charged side chain interaction. J. Biochem. (Tokyo), 121, 1076–1081 (1997).
  • 9) Saito, A., Sako, Y., Usui, M., Azakami, H., and Kato, A., Functional properties of glycosylated lysozyme secreted in Pichia pastoris. Biosci. Biotechnol. Biochem., 67, 2334–2343 (2003).
  • 10) Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685 (1970).
  • 11) Zacharius, R. M., Zell, T. E., Morrison, J. H., and Woodlock, J. J., Glycoprotein staining following electrophoresis on acrylamide gels. Anal. Biochem., 30, 148–152 (1969).
  • 12) Kato, A., Tanimoto, S., Muraki, Y., Oda, Y., Inoue, Y., and Kobayashi, K., Relationships between conformational stabilities and surface functional properties of mutant hen egg-white lysozymes constructed by genetic engineering. J. Agric. Food Chem., 42, 227–230 (1994).
  • 13) Steve, S., and Eric, A. H., Modification of pK values caused by change in H-bond geometry. Proc. Natl. Acad. Sci. USA, 82, 2741–2745 (1985).

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