- 1) Kinsella, J. E., Lokesh, B., Broughton, S., and Whelan, J., Dietary polyunsaturated fatty acids and eicosanoids: potential effects on the modulation of inflammatory and immune cells: an overview. Nutrition, 6, 24–44 (1990).
- 2) Wallis, J. G., Watts, J. L., and Browse, J., Polyunsaturated fatty acid synthesis: what will they think of next? Trends Biochem. Sci., 27, 467–473 (2002).
- 3) Tocher, D. R., Leaver, M. J., and Hodgson, P. A., Recent advances in the biochemistry and molecular biology of fatty acyl desaturases. Prog. Lipid Res., 37, 73–117 (1998).
- 4) Sperling, P., Ternes, P., Zank, T. K., and Heinz, E., The evolution of desaturases. Prostaglandins Leukot. Essent. Fatty Acids, 68, 73–95 (2003).
- 5) Schwartzbeck, J. L., Jung, S., Abbott, A. G., Mosley, E., Lewis, S., Pries, G. L., and Powell, G. L., Endoplasmic oleoyl-PC desaturase references the second double bond. Phytochemistry, 57, 643–652 (2001).
- 6) Sasata, R. J., Reed, D. W., Loewen, M. C., and Covello, P. S., Domain swapping localizes the structural determinants of regioselectivity in membrane-bound fatty acid desaturases of Caenorhabditis elegans. J. Biol. Chem., 279, 39296–39302 (2004).
- 7) Meesapyodsuk, D., Reed, D. W., Savile, C. K., Buist, P. H., Schäfer, U. A., Ambrose, S. J., and Covello, P. S., Substrate specificity, regioselectivity and cryptoregiochemistry of plant and animal ω-3 fatty acid desaturases. Biochem. Soc. Trans., 28, 632–635 (2000).
- 8) Reed, D. W., Schäfer, U. A., and Covello, P. S., Characterization of the Brassica napus extraplastidial linoleate desaturase by expression in Saccharomyces cerevisiae. Plant Physiol., 122, 715–720 (2000).
- 9) Ferrante, G., and Kates, M., Pathways for desaturation of oleoyl chains in Candida lypolytica. Can. J. Biochem. Cell Biol., 61, 1191–1196 (1983).
- 10) Sperling, P., Linscheid, M., Stocker, S., Muhlbach, H. P., and Heinz, E., In vivo desaturation of cis-delta 9-monounsaturated to cis-delta 9,12-diunsaturated alkenylether glycerolipids. J. Biol. Chem., 268, 26935–26940 (1993).
- 11) Los, D. A., and Murata, N., Structure and expression of fatty acid desaturase. Biochim. Biophys. Acta, 1394, 3–15 (1998).
- 12) Wallis, J. G., and Browse, J., Mutants of Arabidopsis reveal many roles for membrane lipids. Prog. Lipid Res., 41, 254–278 (2002).
- 13) Griffiths, G., Stobart, A. K., and Stymne, S., Delta 6- and delta 12-desaturase activities and phosphatidic acid formation in microsomal preparations from the developing cotyledons of common borage (Borago officinalis). Biochem. J., 252, 641–647 (1988).
- 14) Jackson, F. M., Fraser, T. C., Smith, M. A., Lazarus, C., Stobart, A. K., and Griffiths, G., Biosynthesis of C18 polyunsaturated fatty acids in microsomal membrane preparations from the filamentous fungus Mucor circinelloides. Eur. J. Biochem., 252, 513–519 (1998).
- 15) Galle-Le Bastard, A. M., Demandre, C., Oursel, A., Joseph, M., Mazliak, P., and Kader, J. C., Phosphatidylcholine molecular species involved in g-linolenic acid biosynthesis in microsomes from borage seeds. Physiol. Plant., 108, 118–124 (2000).
- 16) Domergue, F., Abbadi, A., Ott, C., Zank, T. K., Zähringer, U., and Heinz, E., Acyl carriers used as substrates by the desaturases and elongases involved in very long-chain polyunsaturated fatty acids biosynthesis reconstituted in yeast. J. Biol. Chem., 278, 35115–35126 (2003).
- 17) Oura, T., and Kajiwara, S., Saccharomyces kluyveri FAD3 encodes an ω3 fatty acid desaturase. Microbiology, 150, 1983–1990 (2004).
- 18) Watanabe, K., Oura, T., Sakai, H., and Kajiwara, S., Yeast Δ12 fatty acid desaturase: gene cloning expression and function. Biosci. Biotechnol. Biochem., 68, 721–727 (2004).
- 19) Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K., “Current Protocols in Molecular Biology,” Green Publishing Associates and Wiley-Interscience John Wiley and Sons, New York (1992).
- 20) Kajiwara, S., Shirai, A., Fujii, T., Toguri, T., Nakamura, K., and Ohtaguchi, K., Polyunsaturated fatty acid biosynthesis in Saccharomyces cerevisiae: expression of ethanol tolerance and the FAD2 gene from Arabidopsis thaliana. Appl. Environ. Microbiol., 62, 4309–4313 (1996).
- 21) Oura, T., and Kajiwara, S., Cloning and functional characterization of a fatty acid synthase component FAS2 gene from Saccharomyces kluyveri. Curr. Genet., 49, 393–402 (2006).
- 22) Fay, L., and Richli, U., Location of double bonds in polyunsaturated fatty acids by gas chromatography-mass spectrometry after 44-dimethyloxazoline derivatization. J. Chromatogr., 541, 89–98 (1991).
Full access
Substrate Specificity and Regioselectivity of Δ12 and ω3 Fatty Acid Desaturases from Saccharomyces kluyveri
Reprints and Corporate Permissions
Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?
To request a reprint or corporate permissions for this article, please click on the relevant link below:
Academic Permissions
Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?
Obtain permissions instantly via Rightslink by clicking on the button below:
If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.
Related research
People also read lists articles that other readers of this article have read.
Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.
Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.