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Bioscience, Biotechnology, and Biochemistry Volume 72, 2008 - Issue 12
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Original Articles

Silencing of Ref-1 Expression by Retrovirus-Mediated shRNA Sensitizes HEK293 Cells to Hydrogen Peroxide-Induced Apoptosis

Jian-Jun XIONGDepartment of Biochemistry and Molecular Biology, Sun Yat-sen University
,
Yan ZHANGDepartment of Biochemistry and Molecular Biology, Sun Yat-sen University
,
Jia-Li WANGDepartment of Biochemistry and Molecular Biology, Sun Yat-sen University
,
Guo-Dong BAODepartment of Biochemistry and Molecular Biology, Sun Yat-sen University
,
Yang ZHANGDepartment of Biochemistry and Molecular Biology, Sun Yat-sen University
&
Zhen-Yu ZHUDepartment of Biochemistry and Molecular Biology, Sun Yat-sen University;Da-An Gene Co., Ltd., of Sun Yat-sen University
Pages 3206-3210 | Received 19 Jun 2008, Accepted 08 Aug 2008, Published online: 22 May 2014

  • 1) Evans, A. R., Limp-Foster, M., and Kelley, M. R., Going APE over ref-1. Mutat. Res., 461, 83–108 (2000).
  • 2) Jayaraman, L., Murthy, K. G., Zhu, C., Curran, T., Xanthoudakis, S., and Prives, C., Identification of redox/repair protein Ref-1 as a potent activator of p53. Genes Dev., 11, 558–570 (1997).
  • 3) Mitomo, K., Nakayama, K., Fujimoto, K., Sun, X., Seki, S., and Yamamoto, K., Two different cellular redox systems regulate the DNA-binding activity of the p50 subunit of NF-kappa B in vitro. Gene, 145, 197–203 (1994).
  • 4) Huang, L. E., Arany, Z., Livingston, D. M., and Bunn, H. F., Activation of hypoxia-inducible transcription factor depends primarily upon redox-sensitive stabilization of its alpha subunit. J. Biol. Chem., 271, 32253–32259 (1996).
  • 5) Hirota, K., Matsui, M., Iwata, S., Nishiyama, A., Mori, K., and Yodoi, J., AP-1 transcriptional activity is regulated by a direct association between thioredoxin and Ref-1. Proc. Natl. Acad. Sci. USA, 94, 3633–3638 (1997).
  • 6) Angkeow, P., Deshpande, S. S., Qi, B., Liu, Y. X., Park, Y. C., Jeon, B. H., Ozaki, M., and Irani, K., Redox factor-1: an extra-nuclear role in the regulation of endothelial oxidative stress and apoptosis. Cell Death Differ., 9, 717–725 (2002).
  • 7) Robertson, K. A., Bullock, H. A., Xu, Y., Tritt, R., Zimmerman, E., Ulbright, T. M., Foster, R. S., Einhorn, L. H., and Kelley, M. R., Altered expression of Ape1/ref-1 in germ cell tumors and overexpression in NT2 cells confers resistance to bleomycin and radiation. Cancer Res., 61, 2220–2225 (2001).
  • 8) Lau, J. P., Weatherdon, K. L., Skalski, V., and Hedley, D. W., Effects of gemcitabine on APE/ref-1 endonuclease activity in pancreatic cancer cells, and the therapeutic potential of antisense oligonucleotides. Br. J. Cancer, 91, 1166–1173 (2004).
  • 9) Yang, S., Irani, K., Heffron, S. E., Jurnak, F., and Meyskens, F. L., Jr., Alterations in the expression of the apurinic/apyrimidinic endonuclease-1/redox factor-1 (APE/Ref-1) in human melanoma and identification of the therapeutic potential of resveratrol as an APE/Ref-1 inhibitor. Mol. Cancer Ther., 4, 1923–1935 (2005).
  • 10) Fan, Z., Beresford, P. J., Zhang, D., Xu, Z., Novina, C. D., Yoshida, A., Pommier, Y., and Lieberman, J., Cleaving the oxidative repair protein Ape1 enhances cell death mediated by granzyme A. Nat. Immunol., 4, 145–153 (2003).
  • 11) Hockenbery, D. M., Oltvai, Z. N., Yin, X. M., Milliman, C. L., and Korsmeyer, S. J., Bcl-2 functions in an antioxidant pathway to prevent apoptosis. Cell, 75, 241–251 (1993).
  • 12) Green, D. R., and Reed, J. C., Mitochondria and apoptosis. Science, 281, 1309–1312 (1998).
  • 13) Kowaltowski, A. J., and Fiskum, G., Redox mechanisms of cytoprotection by Bcl-2. Antioxid. Redox Signal., 7, 508–514 (2005).
  • 14) Martindale, J. L., and Holbrook, N. J., Cellular response to oxidative stress: signaling for suicide and survival. J. Cell. Physiol., 192, 1–15 (2002).
  • 15) Fritz, G., Human APE/Ref-1 protein. Int. J. Biochem. Cell Biol., 32, 925–929 (2000).
  • 16) Ozaki, M., Suzuki, S., and Irani, K., Redox factor-1/APE suppresses oxidative stress by inhibiting the rac1 GTPase. FASEB J., 16, 889–890 (2002).
  • 17) Yoo, Y. H., Lim, Y. J., Park, S. E., Kim, J. M., and Park, Y. C., Overexpression of redox factor-1 negatively regulates NO synthesis and apoptosis in LPS-stimulated RAW 264.7 macrophages. FEBS Lett., 556, 39–42 (2004).
  • 18) Jeon, B. H., Gupta, G., Park, Y. C., Qi, B., Haile, A., Khanday, F. A., Liu, Y. X., Kim, J. M., Ozaki, M., White, A. R., Berkowitz, D. E., and Irani, K., Apurinic/apyrimidinic endonuclease 1 regulates endothelial NO production and vascular tone. Circ. Res., 95, 902–910 (2004).
  • 19) Gross, A., McDonnell, J. M., and Korsmeyer, S. J., BCL-2 family members and the mitochondria in apoptosis. Genes Dev., 13, 1899–1911 (1999).
  • 20) Mahajan, N. P., Linder, K., Berry, G., Gordon, G. W., Heim, R., and Herman, B., Bcl-2 and Bax interactions in mitochondria probed with green fluorescent protein and fluorescence resonance energy transfer. Nat. Biotechnol., 16, 547–552 (1998).
  • 21) Golstein, P., Controlling cell death. Science, 275, 1081–1082 (1997).
  • 22) Miyashita, T., Krajewski, S., Krajewska, M., Wang, H. G., Lin, H. K., Liebermann, D. A., Hoffman, B., and Reed, J. C., Tumor suppressor p53 is a regulator of bcl-2 and bax gene expression in vitro and in vivo. Oncogene, 9, 1799–1805 (1994).
  • 23) Sen, C. K., and Packer, L., Antioxidant and redox regulation of gene transcription. FASEB J., 10, 709–720 (1996).
  • 24) Bentires-Alj, M., Dejardin, E., Viatour, P., Van Lint, C., Froesch, B., Reed, J. C., Merville, M. P., and Bours, V., Inhibition of the NF-kappa B transcription factor increases Bax expression in cancer cell lines. Oncogene, 20, 2805–2813 (2001).
  • 25) Garrido, C., Galluzzi, L., Brunet, M., Puig, P. E., Didelot, C., and Kroemer, G., Mechanisms of cytochrome c release from mitochondria. Cell Death Differ., 13, 1423–1433 (2006).
  • 26) Nunez, G., Benedict, M. A., Hu, Y., and Inohara, N., Caspases: the proteases of the apoptotic pathway. Oncogene, 17, 3237–3245 (1998).

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