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Bioscience, Biotechnology, and Biochemistry Volume 72, 2008 - Issue 12
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Original Articles

A Functional Putative Phytochelatin Synthase from the Primitive Red Alga Cyanidioschyzon merolae

Yuko OSAKIDepartment of Applied Biological Chemistry, School of Agricultural and Life Sciences, The University of Tokyo
,
Tomoo SHIRABEDepartment of Applied Biological Chemistry, School of Agricultural and Life Sciences, The University of Tokyo
,
Shun TAMURADepartment of Applied Biological Chemistry, School of Agricultural and Life Sciences, The University of Tokyo
&
Etsuro YOSHIMURADepartment of Applied Biological Chemistry, School of Agricultural and Life Sciences, The University of Tokyo
Pages 3306-3309 | Received 22 Jul 2008, Accepted 31 Aug 2008, Published online: 22 May 2014

  • 1) Zenk, M. H., Heavy metal detoxification in higher plants: a review. Gene, 179, 17921–17930 (1996).
  • 2) Ruotolo, R., Peracchi, A., Bolchi, A., Infusini, G., Amoresano, A., and Ottonello, S., Domain organization of phytochelatin synthase: functional properties of truncated enzyme species identified by limited proteolysis. J. Biol. Chem., 279, 14686–14693 (2004).
  • 3) Clemens, S., Kim, E. J., Neumann, D., and Schroeder, J. I., Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast. EMBO J., 18, 3325–3333 (1999).
  • 4) Ha, S. B., Smith, A. P., Howden, R., Dietrich, W. M., Bugg, S., O’Connell, M. J., Goldsbrough, P. G., and Cobbett, C. S., Phytochelatin synthase genes from Arabidopsis and the yeast Schizosaccharomyces pombe. Plant Cell, 11, 1153–1163 (1999).
  • 5) Vatamaniuk, O. K., Mari, S., Lu, Y.-P., and Rea, P. A., AtPCS1, a phytochelatin synthase from Arabidopsis: isolation and in vitro reconstitution. Proc. Natl. Acad. Sci. USA, 96, 7110–7115 (1999).
  • 6) Oven, M., Page, J. E., Zenk, M. H., and Kutchan, T. M., Molecular characterization of homo-phytochelatin synthase of soybean Glycine max. J. Biol. Chem., 277, 4747–4754 (2002).
  • 7) Heiss, S., Wachter, A., Bogs, J., Cobbett, C., and Rausch, T., Phytochelatin synthase (PCS) protein is induced in Brassica juncea leaves after prolonged Cd exposure. J. Exp. Bot., 54, 1833–1839 (2003).
  • 8) Clemens, S., Schroeder, J. I., and Denkolb, T., Caenorhabditis elegans expresses a functional phytochelatin synthase. Eur. J. Biochem., 268, 3640–3643 (2001).
  • 9) Cobbett, C. S., Phytochelatins and their roles in heavy metal detoxification. Plant Physiol., 123, 825–832 (2000).
  • 10) Maier, T., Yu, C., Küllertz, G., and Clemens, S., Localization and functional characterization of metal-binding sites in phytochelatin synthases. Planta, 218, 300–308 (2003).
  • 11) Rea, P. A., Vatamaniuk, O. K., and Rigden, D. J., Weeds, worms, and more: papain’s long-lost cousin, phytochelatin synthase. Plant Physiol., 136, 2463–2474 (2004).
  • 12) Harada, E., von Roepenack-Lahaye, E., and Clemens, S. A., Cyanobacterial protein with similarity to phytochelatin synthases catalyzes the conversion of glutathione to γ-glutamyl cysteine and lacks phytochelatin synthase activity. Phytochem., 65, 3179–3185 (2004).
  • 13) Tsuji, N., Nishikori, S., Iwabe, O., Matsumoto, S., Shiraki, K., Miyasaka, H., Takagi, M., Miyamoto, K., and Hirata, K., Comparative analysis of the two-step reaction catalyzed by prokaryotic and eukaryotic phytochelatin synthase by an ion-pair liquid chromatography assay. Planta, 222, 181–191 (2005).
  • 14) Sneller, F. E. C., van Heerwaarden, L. M., Koevoets, P. L. K., Vooijs, R., Schat, H., and Verkleij, J. A. C., Derivatization of phytochelatins from Silene vulgaris, induced upon exposure to arsenate and cadmium: comparison of derivatization with Ellman’s reagent and monobromobimane. J. Agric. Food Chem., 48, 4012–4019 (2000).
  • 15) Grill, E., Winnacker, E.-L., and Zenk, M. H., Phytochelatins, a class of heavy-metal-binding peptides from plants, are functionally analogous to metallothioneins. Proc. Natl. Acad. Sci. USA, 84, 439–443 (1987).
  • 16) Wünschmann, J., Beck, A., Meyer, L., Letzel, T., Grill, E., and Lendzian, K. J., Phytochelatins are synthesized by two vacuolar serine carboxypeptidases in Saccharomyces cerevisiae. FEBS Lett., 581, 1681–1687 (2007).
  • 17) Loeffler, S., Hochberger, A., Grill, E., Winnacker, E.-L., and Zenk, M. H., Termination of the phytochelatin synthase reaction through sequestration of heavy metals by the reaction product. FEBS Lett., 258, 42–46 (1989).
  • 18) Sherman, F., Getting started with yeast. Methods Enzymol., 194, 3–21 (1991).
  • 19) Vatamaniuk, O. K., Mari, S., Lang, A., Chalasani, S., Demkiv, L. O., and Rea, P. A., Phytochelatin synthase, a dipeptidyltransferase that undergoes multisite acylation with γ-glutamylcysteine during catalysis: stoichiometric and site-directed mutagenic analysis of Arabidopsis thaliana PCS1-catalyzed phytochelatin synthase. J. Biol. Chem., 279, 22449–22460 (2004).
  • 20) Romanyuk, N. D., Rigden, D. J., Vatamaniuk, O. K., Lang, A., Cahoon, R. E., Jez, J. M., and Rea, P. A., Mutagenic definition of a papain-like catalytic triad, sufficiency of the N-terminal domain for single-site core catalytic enzyme acylation, and C-terminal domain for augmentative metal activation of a eukaryotic phytochelatin synthase. Plant Physiol., 141, 858–869 (2006).

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