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Expert Opinion on Drug Discovery Volume 11, 2016 - Issue 2
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Reviews

Aptamers: current challenges and future prospects

Guido Tomás RozenblumDepartamento de Investigaciones Biomédicas y Biotecnológicas, Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y Diagnóstico, CEBBAD - Universidad Maimónides, Buenos Aires, ArgentinaCorrespondence[email protected]
,
Vanina Gisela LopezDepartamento de Investigaciones Biomédicas y Biotecnológicas, Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y Diagnóstico, CEBBAD - Universidad Maimónides, Buenos Aires, Argentina
,
Alfredo Daniel VitulloDepartamento de Investigaciones Biomédicas y Biotecnológicas, Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y Diagnóstico, CEBBAD - Universidad Maimónides, Buenos Aires, Argentina
&
Martín RadrizzaniLaboratorio de Neuro y Citogenética Molecular, Centro de Estudios de Salud y Medio Ambiente, Universidad de San Martín – CONICET, Buenos Aires, Argentina
Pages 127-135 | Received 22 Oct 2015, Accepted 20 Nov 2015, Published online: 18 Dec 2015

References

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•• The authors describe the systematic evolution of ligands by exponential enrichment process for aptamer discovery and generated a table of aptamers developed until 2007. This review should be a starting point for those who are new in the aptamer field.

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• This review offers a comprehensive lecture about the great potential of aptamers as biosensors and should be read to understand how aptamers can influence in the nanotechnology field.

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• The first author was the first to describe the systematic evolution of ligands by exponential enrichment process and he is the founder of Somalogic. In this article, the authors applied their slow off-rate modified aptamer technology for the development of a proteomic assay to identify clinically relevant biomarkers.

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•• This review describes the usage of modified nucleotides in the context of aptamers. It is a recommended lecture for those who are new in the field of aptamers and wants to understand how modified nucleotides can lead to novel aptamer-based technologies.

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•• This review is highly suggested to understand one of the technologies that can lead to a solid high-throughput and automated aptamer discovery platform. The authors describe current technologies for the generation of DNA microarrays and those that can be adapted for aptamer discovery are mentioned.

•• This review is highly suggested to understand the technology behind next-generation sequencing systems. The authors explain in a comprehensive fashion Illumina’s and Life Technologies system among others.

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