38
Views
36
CrossRef citations to date
0
Altmetric
Review

Molecular profiling of tissue samples using laser capture microdissection

&
Pages 53-60 | Published online: 09 Jan 2014

References

  • Going J, Lamb RF. Practical histological microdissection for PCR analysis. J. Pathol. 179, 121–124 (1996).
  • Shibata D, Hawes D, Li ZH, Hernandez AM, Spruck CH, Nichols PW. Specific genetic analysis of microscopic tissue after selective ultraviolet radiation fractionation and the polymerase chain reaction. Am. J. Pathol. 141(3), 539–543 (1992).
  • Emmert-Buck MR, Bonner RF, Smith PD et al. Laser capture microdissection. Science 274, 998–1001(1996).
  • • This is the original paper describing the technique of LCM and its development and capabilities.
  • Fend F, Emmert-Buck MR, Chuaqui R et al. Immuno-LCM: laser capture microdissection of immunostained frozen sections for mRNA analysis. Am. J. Pathol. 154(1), 61–66 (1999).
  • Kasai T, Shimajiri S, Hashimoto H. Detection of SYT-SSX fusion transcripts in both epithelial and spindle cell areas of biphasic synovial sarcoma using laser capture microdissection. Mol. Pathol. 53(2), 107–110 (2000).
  • Culp LA, Lin WC, Kleinman NR. Tagged tumor cells reveal regulatory steps during earliest stages of tumor progression and micrometastasis. Histol. Histopathol. 14(3), 879–886 (1999).
  • Murakami H, Liotta L, Star RA. IF-LCM: laser capture microdissection of immunofluorescently defined cells for mRNA analysis. Kidney Int. 58(3), 1346–1553 (2000).
  • Jones C, Foschini MP, Chaggar R et al. Comparative genomic hybridization analysis of myoepithelial carcinoma of the breast. Lab. Invest. 80(6), 831–836 (2000).
  • Luo L, Salunga RC, Guo H et al. Gene expression profiles of laser-captured adjacent neuronal subtypes. Nature Med. 5(1), 117–122 (1999).
  • •• This is one of the first papers describing the potential of LCM to demonstrate differential gene expression from single cell populations.
  • Suarez-Quian CA, Goldstein SR, Pohida T et al. Laser capture microdissection of single cells from complex tissues. Biotechniques 26, 328–335 (1999).
  • Schutze K, Posl H, Lahr G. Laser micromanipulation systems as universal tools in cellular and molecular biology and in medicine. Cell. Mol. Biol. 44(5), 735– 746 (1998).
  • Leethanakul C, Patel V, Gillespie J et al. Gene expression profiles in squamous cell carcinoma of the oral cavity: use of laser capture microdissection for the construction and analysis of stage-specific cDNA libraries. Oral Oncology 36, 474–483 (2000).
  • Krizman DB, Chuaqui RF, Meltzer PS et al. Construction of a representative cDNA library from prostatic intraepithelial neoplasia (PIN). Cancer Res. 56(23), 5380–5383 (1996).
  • Datson NA, van der Perk-de Jong J, van den Berg MP, de Kloet ER, Vreugdenhil E. MicroSAGE: a modified procedure for serial analysis of gene expression in limited amounts of tissue. Nucleic Acids Res. 27(5), 1300–1307 (1999).
  • Strausberg RL, Buetow KH, Emmert-Buck MR, Klausner RD. The cancer genome anatomy project: building an annotated gene index. Trends Genet. 16, 103–106 (2000).
  • • This paper describes the molecular profiling concept in the context of a comprehensive genome-based approach to the study of human cancer.
  • Sgroi DC, Teng S, Robinson G, LeVangie R, Hudson JR, Jr., Elkahloun AG. In vivo gene expression profile analysis of human breast cancer progression. Cancer Res. 59(22), 5656–5661 (1999).
  • Ohyama H, Zhang X, Kohno Y et al. Laser capture microdissection-generated target sample for high-density oligonucleotide array hybridization. Biotechniques 29(3), 530–536 (2000).
  • Leethanakul C, Patel V, Gillespie J et al. Distinct pattern of expression of differentiation and growth-related genes in squamous cell carcinomas of the head and neck revealed by the use of laser capture microdissection and cDNA arrays. Oncogene 19, 3220–3224 (2000).
  • Mora J, Akram M, Cheung NK, Chen L, Gerald WL. Laser capture microdissected schwannian and neuroblastic cells in stage 4 neuroblastomas have the same genetic alterations. Med. Pediatr. Oncol. 35(6), 534–537 (2000).
  • Kohda Y, Murakami H, Moe OW, Star RA. Analysis of segmental renal gene expression by laser capture microdissection. Kidney Int. 57(1), 321–331 (2000).
  • Slebos RJ, Hoppin JA, Tolbert PE et al. Kras and p53 in pancreatic cancer: association with medical history, histopathology and environmental exposures in a population-based study. Cancer Epidemiol. Biomarkers Prev. 9(11), 1223–1232 (2000).
  • Sirivatanauksorn Y, Sirivatanauksorn V, Bhattacharya S et al. Evolution of genetic abnormalities in hepatocellular carcinomas demonstrated by DNA fingerprinting. J. Pathol. 189(3), 344–350 (1999).
  • Patel AC, Anna CH, Foley JF et al. Hypermethylation of the p16 (Ink4a) promoter in B6C3F1 mouse primary lung adenocarcinomas and mouse lung cell lines. Carcinogenesis 21(19), 1691–1700 (2000).
  • Grosse J, Bulling A, Brucker C et al. Synaptosome-associated protein of 25 kilodaltons in oocytes and steroidproducing cells of rat and human ovary: molecular analysis and regulation by gonadotropins. Biol. Reprod. 63(2), 643– 650 (2000).
  • Gillespie JW, Nasir A, Kaiser HE. Loss of heterozygosity in papillary and follicular thyroid carcinoma: a mini review. In vivo 14(1), 139–140 (2000).
  • Lutchman M, Pack S, Kim AC et al. Loss of heterozygosity on 8p in prostate cancer implicates a role for dematin in tumor progression. Cancer Genet. Cytogenet. 115(1), 65–69 (2000).
  • Milchgrub S, Wistuba I, Kim BK et al. Molecular identification of metastatic cancer to the skin using laser capture microdissection: a case report. Cancer 88(4), 749–754 (2000).
  • Verhagen PC, Zhu XL, Rohr LR et al. Microdissection, DOP-PCR and comparative genomic hybridization of paraffin-embedded familial prostate cancers. Cancer Genet. Cytogenet. 122(1), 43–48 (2000).
  • Emmert-Buck MR, Gillespie JW, Paweletz CP et al. An approach to proteomic analysis of human tumors. Mol. Carcinog. 27, 158–165 (2000).
  • Banks RE, Dunn MJ, Forbes MA et al. The potential use of laser capture microdissection to selectively obtain distinct populations of cells for proteomic analysis preliminary findings. Electrophoresis 20(4–5), 689–700 (1999).
  • Ornstein DK, Gillespie JW, Paweletz CP et al. Proteomic analysis of laser capture microdissected prostate cancer and in vitro cell lines. Electrophoresis 21(11), 2235–2242 (2000).
  • von Eggeling F, Davies H, Lomas L et al. Tissue-specific microdissection coupled with ProteinChip array technologies: applications in cancer research. Biotechniques 29, 1066–1070 (2000).
  • Shibutani M, Unemaya C, Miyazaki K, Toyoda K, Hirose M. Methacarn fixation: a novel tool for analysis of gene expressions in paraffin-embedded tissue specimens. Lab. Invest. 80(2), 199–208 (2000).
  • Goldsworthy SM, Stockton PS, Trempus CS, Foley JF, Maronpot RR. Effects of fixation on RNA extraction and amplification from laser capture microdissected tissue. Mol. Carcinog. 25(2), 86–91 (1999).
  • Maitra A, Wistuba I, Gibbons D, Gazdar AF, Albores-Saavedra J. Allelic losses at chromosome 3p are seen in human papilloma virus 16 associated transitional cell carcinoma of the cervix. Gynecol. Oncol. 74(3), 361–368 (1999).
  • Englert CR, Baibakov GV, Emmert-Buck MR. Layered expression scanning: rapid molecular profiling of tumor samples. Cancer Res. 60, 1526–1530 (2000).
  • Alizadeh AA, Eisen MB, Davis RE et al. Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling. Nature 403, 503–511 (2000).
  • •• This paper demonstrates the significant capacity of molecular profiling to generate clinically relevant information about specific subtypes of disease.
  • Cole KA, Chuaqui RF, Katz K et al. cDNA sequencing and analysis of PB39: a novel gene up-regulated in prostate cancer. Genomics 51(2), 282–287 (1998).

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.