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Review

Immunofluorescence versus ELISA for the detection of antinuclear antigens

Pages 226-232 | Published online: 09 Jan 2014

References

  • Osterland CK. Laboratory diagnosis and monitoring in chronic systemic autoimmune diseases. Clin. Chem. 40, 2146–2154 (1994).
  • Moder KG. Use and interpretation of rheumatologic tests: a guide for clinicians. Mayo Clin. Proc. 71, 391–396 (1996).
  • Field M, Williams DG, Charles R Maini RN. Specificity of antiSm antibodies by ELISA for systemic lupus erythematosus: increased sensitivity of detection using purified peptide antigens. Ann. Rheum. Dis. 47,820-825 (1988).
  • Vazquez-Abad D, Rothfield NF. Sensitivity and specificity of antiJol antibodies in autoimmune diseases with myositis. Arthritis Rheum. 39,292–296 (1996).
  • Hochberg MC. Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 40, 1725 (1997).
  • Alaryon-Segovia D. Mixed connective tissue disease and overlap syndromes. Chn. Dermatol 12, 309–316 (1994).
  • Vitali C, Bombardieri S, Moutsopoulos HM et al. Assessment of the European classification criteria for Sjogren's syndrome in a series of clinically defined cases: results of a prospective rnulticentre study. Ann. Rheum. Dig. 55, 116–121 (1996).
  • Rondeel JMM, van Gelder W, van der Leeden H, Dinkelaar RB. Different strategies in the laboratory diagnosis of autoimmune disease: immunofluorescence, enzyme-linked immunosorbent assay or both? Ann. Chn. Biochem. 36, 189–195 (1999).
  • •Predictive values and costs of different strategies in the cascade testing of ANA in a general hospital laboratory are studied. Both technical and clinical performances of tests are considered.
  • Adams BB, Mutasim DE The diagnostic value of antinuclear antibody testing. Int. Dermatol 39, 887–891 (2000).
  • •Short review of the clinical usefulness of ANA testing for the nonexpert reader.
  • Ulvestad E, Kanestrom A, Madland TM, Thomassen E, Haga HJ, Vollset SE Evaluation of diagnostic tests for antinudear antibodies in rheumatological practice. Scand Immund 52, 309–315 (2000).
  • ••Good example of how ANA tests shouldbe evaluated, considering both technical and clinical criteria. Paper discusses the influence of cut-off values on performance characteristics.
  • Ward MM. Laboratory testing for systemic rheumatic diseases. Postgrari Med. 103, 93–100 (1998).
  • Malleson PN, Sailer M, Mackinnon MJ. Usefulness of antinuclear antibody testing to screen for rheumatic diseases. Arch. Dig. Child 77, 299–304 (1997).
  • Sontheimer RD, McCauliffe DP, Zappi E, Targoff I. Antinuclear antibodies: clinical correlations and biologic significance. Adv. Dermatol 7, 3–52 (1991).
  • Homburger HA. Cascade testing for autoantibodies in connective tissue diseases. Mayo Chn. Proc. 70, 183–184 (1995).
  • Tan EM, Feltkamp TEW, Smolen JS et al. Range of antinuclear antibodies in 'healthy' individuals. Arthritis Rheum. 40, 1601–1611 (1997).
  • ••Classical paper discussing the value of chosing the right cut-off or titer and its influence on the number of false-positives.
  • Fritzler MJ, Pauls JD, Kinsella TD, Bowen TJ. Antinuclear, anticytoplasmic and antiSjögren's syndrome antigen A (SS-A/ Ro) antibodies in female blood donors. Chn. Immunot Immunopathol 36, 120–128 (1985).
  • Pollock W, Toh BH. Routine immunofluorescence detection of Ro/SS-A autoantibody using HEp-2 cells transfected with human 60 kDa Ro/SS-A. j Chn. Pathol 52, 684–687 (1999).
  • Rondeel JMM, Klootwijk W, Linkels E, de Greef WJ, Visser TJ. Neural differentiation of the human neuroblastoma cell line IMR32 induces production of a thyrotropin-releasing hormone-like peptide. Brain Res. 665, 262–268 (1994).
  • Hoffman GS, Specks U. Antineutrophil cytoplasmic antibodies. Arthritis Rheum. 41, 1521–1537 (1998).
  • Thomas C, Robinson JA. The antinuclear antibody test. When is a positive result clinically relevant? Postgrad. Med. 94, 55–66 (1993).
  • Vlachoyiannopoulos PG, Tzavara V, Dafni U, Spanos E, Moutsopoulos HM. Clinical features and evolution of antinuclear antibody positive individuals in a rheumatology out-patient clinic. J Rheumatol. 25, 886–891 (1998).
  • Jitsukawa T, Nakajima S, Usui J, Watanabe H. Detection of antinuclear antibodies from patients with systemic rheumatic diseases by ELISA using HEp-2 cell nuclei. J. Clin. Lab. Anal. 5, 49–53 (1991).
  • Lehmann HP, Fuhling I, Ott C, Hudepohl B, Haass M. HEp2 ANA ETA: a new fully automated assay for the screening of antinuclear antibodies. Isr. Med. Assoc. J. 2, 646–648 (2000).
  • McCauliffe DP, Wang L, Satoh M, Reeves WH, Small D. Recombinant 52 kDa Ro(SSA) ELISA detects autoantibodies in Sjogren's syndrome sera that go undetected by conventional serologic assays. J Rheumatol. 24, 860–866 (1997).
  • Alem M, Moghadam S, Malki J, Zaidi A, Nayak N, Li TM. Detection of autoantibodies to nuclear antigens by ETA and IF techniques. Allerg. Immunot 29, 191–194 (1997).
  • Cordiali Fei P, D'Agosto G, Ameglio F et al. Determination of antibodies to extractable nuclear antigens with commercial kits: a multicenter study. Int. J. Chn. Lab. Res. 28, 29–33 (1998).
  • •One of the few multicenter studies comparing different ELISAs and western blot.
  • Kapogiannis B, Gussin HA, Teodorescu MR, Teodorescu M. Differences in clinical sensitivity of ELISA tests for autoantibodies with human and bovine extractable nuclear antigens. Lupus 9, 343–352 (2000).
  • James K, Meek G. Evaluation of commercial enzyme immunoassays compared to immunofluorescence and double diffusion for autoantibodies associated with autoimmune disease. Am. J. Clin. Pathol 97, 559–565 (1992).
  • Gonzalez C, Martin T, Arroyo T, Garcia- Isidoro M, Navajo J, Gonzalez-Buitrago J. Comparison and variation of different methodologies for the detection of autoantibodies to nuclear antigens (ANA). J. Clin. Lab. Anal. 11, 388–392 (1997).
  • Gniewek RA, Sandbulte C, Fox P Comparison of antinuclear antibody testing methods by ROC analysis with reference to disease diagnosis. Chn. Chem. 43, 1987–1989 (1997).
  • •One of the few studies using ROC analysis as parameter of performance.
  • Ernlen W, O'Neill L. Clinical siginificance of antinuclear antibodies: comparison of detection with immunofluorescence and enzyme-linked immunosorbent assays. Arthritis Rheum. 40, 1612–1618 (1997).
  • Homburger HA, Cahen YD, Griffiths J, Jacob GL. Detection of antinuclear antibodies: comparative evaluation of enzyme immunoassay and indirect immunofluorescence methods. Arch. Pathol. Lab. Med. 122, 993–999 (1998).
  • Tan EM, Smolen JS, McDougal JS et al. A critical evaluation of enzyme immunoassays for detection of antinuclear autoantibodies of defined specificities. I. Precision, sensitivity and specificity. Arthritis Rheum. 42, 455–464 (1999).
  • •One of the few studies in which a large number of different ELISA kits were compared.
  • Egner W The use of laboratory tests in the diagnosis of SLE.J Clin. Pathol 53,424–432 (2000).
  • Nossent H, Rekvig OP Antinuclear antibody screening in this new millennium: farewell to the microscope? Scancl Rheumatol 30,123–126 (2001).
  • Bayer PM, Fabian B, Htibl W Immunofluorescence assays (IFA) and enzyme-linked immunosorbent assays (ELISA) in autoimmune disease diagnostics—technique, benefits, limitations and applications. Scand. Clin. Lab. Invest. 235, (Suppl.) 68–76 (2001)
  • Reiff A, Inderlied C, Haubruck H, Amos M. Evaluation of a recombinant antigen enzyme linked immunosorbent assay (ELISA) in the diagnostics of antinuclear antibodies (ANA) in children with rheumatic disorders. Arthritis Rheum. 43, S358 (2000).
  • Ulvestad E. Performance characteristics and clinical utility of a hybrid ELISA for detection of ANA. APMIS 109, 217–222 (2001).
  • Kumagai S, Hayashi N. Immunofluorescence-still the 'gold standard' in ANA testing? Scand. j Gun. Lab. Invest. 235,61, (Suppl.) 77–83 (2001).
  • •Recent study discussing the use of the fully automated Cobas Core ELISA.
  • Feltkamp TE. Antinuclear antibody determination in a routine laboratory. Ann. Rheum. Dis. 55,723–727 (1996).
  • Kavanaugh A, Tomar R, Reveille J, Solomon DH, Homburger HA. Guidelines for clinical use of the antinuclear antibody test as tests for specific autoantibodies to nuclear antigens. American College of Pathologists. Arch. Pathol Lab. Med. 124, 71–81 (2000).
  • Mutasim DF, Adams BB. A practical guide for serologic evaluation of autoimmune connective tissue diseases. J Am. Acrid Derrnatol 42,159–174 (2000).

Websites

  • Barland P, Wachs J. Measurement and clinical significance of antinuclear antibodies. In: UpToDate online 9.3 (www.uptodateonline.corn)
  • ••Updated review with summary tables and reference list for the non-expert reader.

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