References
- Davies E, Stankovi B, Azama K, et al. Novel components of the plant cytoskeleton: a beginning to plant ‘cytomics’. Plant Sci 2001;160(2):185-96
- Bengtsson M, Ståhlberg A, Rorsman P, Kubista M. Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels. Genome Res 2005;15(10):1388-92
- Dar RD, Razooky BS, Singh A, et al. Transcriptional burst frequency and burst size are equally modulated across the human genome. Proc Nat Acad Sci USA 2012;109(43):17454-9
- Raj A, Peskin CS, Tranchina D, et al. Stochastic mRNA synthesis in mammalian cells. PLoS Biol 2006;4(10):e309
- Wills QF, Livak KJ, Tipping AJ, et al. Single-cell gene expression analysis reveals genetic associations masked in whole-tissue experiments. Nat Biotechnol 2013;31(8):748-52
- Chubb JR, Trcek T, Shenoy SM, Singer RH. Transcriptional pulsing of a developmental gene. Curr Biol 2006;16(10):1018-25
- Sigal A, Milo R, Cohen A, et al. Variability and memory of protein levels in human cells. Nature 2006;444(7119):643-6
- Kalisky T, Blainey P, Quake SR. Genomic analysis at the single-cell level. Annu Rev Genet 2011;45:431-15
- Larson DR, Singer RH, Zenklusen D. A single molecule view of gene expression. Trends Cell Biol 2009;19(11):630-7
- Wang D, Bodovitz S. Single-cell analysis: the new frontier in ‘omics’. Trends Biotechnol 2010;28(6):281-90
- Galler K, Bräutigam K, Große C, et al. Making a big thing of a small cell – recent advances in single cell analysis. Analyst 2014;139(6):1237-73
- Bendall SC, Nolan GP. From single cells to deep phenotypes in cancer. Nat Biotechnol 2012;30(7):639-47
- Shapiro E, Biezuner T, Linnarsson S. Single-cell sequencing-based technologies will revolutionize whole-organism science. Nat Rev Genet 2013;14(9):618-30
- http://www.worthington-biochem.com/tissuedissociation/
- Ståhlberg A, Bengtsson M. Single-cell gene expression profiling using reverse transcription quantitative real-time PCR. Methods 2010;50(4):282-8
- Pazzagli F, Malentacchi L, Simi C, et al. SPIDIA-RNA: first external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses. Methods 2013;59(1):20-31
- Levesque MJ, Raj A. Single-chromosome transcriptional profiling reveals chromosomal gene expression regulation. Nat Methods 2013;10(3):246-8
- Larsson C, Grundberg I, Söderberg O, Nilsson M. In situ detection and genotyping of individual mRNA molecules. Nat Methods 2010;7(5):395-7
- Gründemann J, Schlaudraff F, Haeckel O, Liss B. Elevated a-synuclein mRNA levels in individual UV-laser-microdissected dopaminergic substantia nigra neurons in idiopathic Parkinson’s disease. Nucleic Acids Res 2008;36(7):e38
- eBioscience. Available from: http://www.ebioscience.com/
- Merck Millipore. Available from: https://www.millipore.com/
- Fabbri F, Carloni S, Zoli W, et al. Detection and recovery of circulating colon cancer cells using a dielectrophoresis-based device: KRAS mutation status in pure CTCs. Cancer Lett 2013;335(1):225-31
- Ståhlberg A, Rusnakova V, Kubista M. The added value of single-cell gene expression profiling. Brief Funct Genomics 2013;12(2):81-9
- Petersson F, Aberg L, Swärd-Nilsson AM, Laurell T. Free flow acoustophoresis: microfluidic-based mode of particle and cell separation. Anal Chem 2007;79(14):5117-23
- Ståhlberg A, Håkansson J, Xian X, et al. Properties of the reverse transcription reaction in mRNA quantification. Clin Chem 2004;50(3):509-15
- Ståhlberg A, Kubista M, Pfaffl M. Comparison of reverse transcriptases in gene expression analysis. Clin Chem 2004;50(9):1678-80
- Ståhlberg A, Rusnakova V, Forootan A, et al. RT-qPCR work-flow for single-cell data analysis. Methods 2013;59(1):80-8
- Tichopad A, Kitchen R, Riedmaier I, et al. Design and optimization of reverse-transcription quantitative PCR experiments. Clin Chem 2009;55(10):1816-23
- Livak KJ, Wills QF, Tipping AJ, et al. Methods for qPCR gene expression profiling applied to 1440 lymphoblastoid single cells. Methods 2013;59(1):71-9
- Svec D, Rusnakova V, Korenkova V Kubista K. Dye-based high-throughput qPCR in microfluidic platform biomark. PCR technology: current innovations. 3rd Edition) 2013;23:323-37
- Rusnakova V, Honsa P, Dzamba D, et al. Heterogeneity of astrocytes: from development to injury - single cell gene expression. PLoS One 2013;8(8):e69734
- Kubista M, Andrade JM, Bengtsson M, et al. The real-time polymerase chain reaction. Mol Aspects Med 2006;27(2-3):95-125
- Bengtsson M, Hemberg M, Rorsman P, Ståhlberg A. Quantification of mRNA in single cells and modelling of RT-qPCR induced noise. BMC Mol Biol 2008;9:63
- Lind K, Ståhlberg A, Zoric N, Kubista M. Combining sequence-specific probes and DNA binding dyes in real-time PCR for specific nucleic acid quantification and melting curve analysis. Biotechniques 2006;40(3):315-19
- Svec D, Andersson D, Pekny M, et al. Direct cell lysis for single-cell gene expression profiling. Front Oncol 2013;3:274
- Bergkvist A, Rusnakova V, Sindelka R, et al. Gene expression profiling – clusters of possibilities. Methods 2010;50(4):323-35
- Tang F, Hajkova P, Barton SC, et al. MicroRNA expression profiling of single whole embryonic stem cells. Nucleic Acids Res 2006;34(2):e9
- Fredriksson S, Gullberg M, Jarvius J, et al. Protein detection using proximity-dependent DNA ligation assays. Nat Biotechnol 2002;20(5):473-7
- Thorsen SB, Lundberg M, Villablanca A, et al. Detection of serological biomarkers by proximity extension assay for detection of colorectal neoplasias in symptomatic individuals. J Transl Med 2013;11(1):253
- Ståhlberg A, Thomsen C, Ruff D, Åman P. Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell. Clin Chem 2012;58(12):1682-91
- Kantlehner M, Kirchner R, Hartmann P, et al. A high-throughput DNA methylation analysis of a single cell. Nucleic Acids Res 2011;39(7):e44
- Bustin SA, Benes V, Garson JA, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 2009;55(4):611-22
- Alberts B, Bray D, Lewis J, et al. Molecular Biology of the Cell. Edition 2 Garland Publishing, Inc; NY, USA: 1989
- Vickaryous MK, Hall BK. Human cell type diversity, evolution, development, and classification with special reference to cells derived from the neural crest. Biol Rev Camb Philos Soc 2006;81(3):425-55
- Lee JT, Bartolomei MS. X-inactivation, imprinting, and long noncoding RNAs in health and disease. Cell 2013;152(6):1308-23
- Meyer KB, Maia AT, O’Reilly M, et al. Allele-specific up-regulation of FGFR2 increases susceptibility to breast cancer. PLoS Biol 2008;6(5):e108
- Picelli S, Björklund ÅK, Faridani OR, et al. Smart-seq2 for sensitive full-length transcriptome profiling in single cells. Nat Methods 2013;10(11):1096-8
- http://www.nanostring.com
- Fu GK, Hu J, Wang PH, Fodor SP. Counting individual DNA molecules by the stochastic attachment of diverse labels. Proc Natl Acad Sci USA 2011;108(22):9026-31
- Huggett JF, Foy CA, Benes V, et al. The digital MIQE guidelines: minimum Information for Publication of Quantitative Digital PCR Experiments. Clin Chem 2013;59(6):892-902
- Ståhlberg A, Andersson D, Aurelius J, et al. Defining cell populations with single-cell gene expression profiling: correlations and identification of astrocyte subpopulations. Nucleic Acids Res 2011;39(4):e24