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Special Report

Relieving the first bottleneck in the drug discovery pipeline: using array technologies to rationalize membrane protein production

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Pages 501-505 | Published online: 09 Jan 2014

References

  • Schlyer S, Horuk R. I want a new drug: G-protein-coupled receptors in drug development. Drug Discov. Today11(11–12), 481–493 (2006).
  • Forstner M, Leder L, Mayr LM. Optimization of protein expression systems for modern drug discovery. Expert Rev. Proteomics4(1), 67–78 (2007).
  • Bonander N, Hedfalk K, Larsson C et al. Design of improved membrane protein production experiments: quantitation of the host response. Protein Science14(7), 1729–1740 (2005).
  • Martinez Molina D, Cornvik T, Eshaghi S et al. Engineering membrane protein overproduction in Escherichia coli. Protein Sci.17(4), 673–680 (2008).
  • Bill RM. Yeast – a panacea for the structure-function analysis of membrane proteins? Curr. Genet.40(3), 157–171 (2001).
  • Wagner S, Baars L, Ytterberg AJ et al. Consequences of membrane protein overexpression in Escherichia coli. Mol. Cell. Proteomics6(9), 1527–1550 (2007).
  • Wagner S, Klepsch MM, Schlegel S et al. Tuning Escherichia coli for membrane protein overexpression. Proc. Natl Acad. Sci. USA105(38), 14371–14376 (2008).
  • Sevastsyanovich Y, Alfasi S, Cole J. Recombinant protein production: a comparative view on host physiology. New Biotechnol.25(4), 175–180 (2009).
  • Bonander N, Darby RA, Grgic L et al. Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield. Microb. Cell. Fact.8, 10 (2009).
  • Ahmed FE. Microarray RNA transcriptional profiling: part I. Platforms, experimental design and standardization. Expert Rev. Mol. Diagn.6(4), 535–550 (2006).
  • Bonander N, Ferndahl C, Mostad P et al. Transcriptome analysis of a respiratory Saccharomyces cerevisiae strain suggests the expression of its phenotype is glucose insensitive and predominantly controlled by Hap4, Cat8 and Mig1. BMC Genomics9, 365 (2008).
  • Gasch AP, Spellman PT, Kao CM et al. Genomic expression programs in the response of yeast cells to environmental changes. Mol. Biol. Cell.11(12), 4241–4257 (2000).
  • Serrano R, Ruiz A, Bernal D, Chambers JR, Arino J. The transcriptional response to alkaline pH in Saccharomyces cerevisiae: evidence for calcium-mediated signalling. Mol. Microbiol.46(5), 1319–1333 (2002).
  • Sahara T, Goda T, Ohgiya S. Comprehensive expression analysis of time-dependent genetic responses in yeast cells to low temperature. J. Biol. Chem.277(51), 50015–50021 (2002).
  • DeRisi JL, Iyer VR, Brown PO. Exploring the metabolic and genetic control of gene expression on a genomic scale. Science278(5338), 680–686 (1997).
  • David NE, Gee M, Andersen B et al. Expression and purification of the Saccharomyces cerevisiae α-factor receptor (Ste2p), a 7-transmembrane-segment G protein-coupled receptor. J. Biol. Chem.272(24), 15553–15561 (1997).
  • Andre N, Cherouati N, Prual C et al. Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen. Protein Sci.15(5), 1115–1126 (2006).
  • Griffith DA, Delipala C, Leadsham J, Jarvis SM, Oesterhelt D. A novel yeast expression system for the overproduction of quality-controlled membrane proteins. FEBS Lett.553(1–2), 45–50 (2003).
  • De Schutter K, Lin YC, Tiels P et al. Genome sequence of the recombinant protein production host Pichia pastoris. Nature Biotechnol.27(6), 561–566 (2009).

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