DETECTION OF GIARDIA LAMBLIA, CRYPTOSPORIDIUM SPP. AND ENTAMOEBA HISTOLYTICA IN CLINICAL STOOL SAMPLES BY USING MULTIPLEX REAL-TIME PCR AFTER AUTOMATED DNA ISOLATION

REFERENCES

• ten Hove R, Schuurman T, Kooistra M, Moller L, van Lieshout L, Verweij JJ. Detection of diarrhoea-causing protozoa in general practice patients in the netherlands by multiplex real-time per. Clin Microbiol Infect 2007; 13: 1001–1007.
   PubMed Web of Science ®Google Scholar
• Fischer Walker CL, Sack D, Black RE. Etiology of diarrhea in older children, ado-lescents and adults: A systematic review. PLoS Negl Trop Dis 2010; 4: e768.
   PubMed Web of Science ®Google Scholar
• van Lieshout L, Verweij JJ. Newer diagnostic approaches to intestinal protozoa. Curr Opin Infect Dis 2010; 23: 488–493.
   Web of Science ®Google Scholar
• Collinet-Adler S, Ward HD. Cryptosporidiosis: Environmental, therapeutic, and preventive challenges. Eur J Clin Microbiol Infect Dis 2010; 29: 927–935.
   Web of Science ®Google Scholar
• Stanley SL, Jr. Amoebiasis. Lancet 2003; 361: 1025–1034.
   PubMed Web of Science ®Google Scholar
• Fotedar R, Stark D, Beebe N, Marriott D, Ellis J, Harkness J. Laboratory diagnostic techniques for entamoeba species. Clin Microbiol Rev 2007; 20:511–532.
   PubMed Web of Science ®Google Scholar
• van Doornum GJ, Guldemeester J, Osterhaus AD, Niesters HG. Diagnosing her-pesvirus infections by real-time amplification and rapid culture. J Clin Microbiol 2003; 41:576–580.
   PubMed Web of Science ®Google Scholar
• Verweij JJ, Blange RA, Templeton K, et al. Simultaneous detection of entamoeba histolytica, giardia lamblia, and cryptosporidium parvum in fecal samples by using multiplex real-time per. J Clin Microbiol 2004; 42: 1220–1223.
   PubMed Web of Science ®Google Scholar
• Guy RA, Payment P. Krull UJ, Horgen PA. Real-time per for quantification of giardia and cryptosporidium in environmental water samples and sewage. App! Environ Microbiol 2003; 69:5178–5185.
   PubMed Web of Science ®Google Scholar
• van Zanten E. Wisselink GJ, de Boer W, Stoll S, Alvarez R, Kooistra-Smid AM. Comparison of the qiasymphony automated nucleic acid extraction and per setup platforms with nuclisens easymag and corbett cas-1200 liquid handling station for the detection of enteric pathogens in fecal samples. J Microbiol Methods 2011; 84:335–340.
   Web of Science ®Google Scholar
• Bruijnesteijn van Coppenraet LE, Wallinga JA, Ruijs GJ, Bruins MJ, Verweij JJ. Parasitological diagnosis combining an internally controlled real-time per assay for the detection of four protozoa in stool samples with a testing algorithm for microscopy. Clin Microbiol Infect 2009; 15: 869–874.
   PubMed Web of Science ®Google Scholar
• Visser LG, Verweij JJ, Van Esbroeck M, Edeling WM, Clerinx J, Polderman AM. Diagnostic methods for differentiation of entamoeba histolytica and enta-moeba dispar in carriers: Performance and clinical implications in a non-endemic setting. Int J Med Microbiol 2006; 296: 397–403.
   PubMed Web of Science ®Google Scholar
• Jackson TF. Entamoeba histolytica and entamoeba dispar are distinct species; clinical, epidemiological and serological evidence. Int J Parasitol 1998; 28:181–186.
   PubMed Web of Science ®Google Scholar
• de Boer RF, Ott A, Kesztyus B, Kooistra-Smid AM. Improved detection of five major gastrointestinal pathogens by use of a molecular screening approach. J Clin Microbiol 2010; 48: 4140–4146.
   PubMed Web of Science ®Google Scholar
• de Wit MA, Koopmans MP, Kortbeek LM, van Leeuwen NJ, Vinje J, van Duyn-hoven YT. Etiology of gastroenteritis in sentinel general practices in the neth-erlands. Clin Infect Dis 2001; 33: 280–288.
   PubMed Web of Science ®Google Scholar
• Yoder JS, Harral C, Beach MJ. Cryptosporidiosis surveillance - united states, 2006-2008. MMWR Surveil/ Summ 2010; 59: 1–14.
   Google Scholar
• Stark D, Al-Qassab SE, Barratt JL, et al. Evaluation of multiplex tandem real-time per for detection of cryptosporidium spp., dientamoeba fragilis, entamoeba histolytica, and giardia intestinalis in clinical stool samples. J Clin Microbiol 2011; 49: 257–262.
   PubMed Web of Science ®Google Scholar
• Swaminathan A, Torresi J, Schlagenhauf P. et al. A global study of pathogens and host risk factors associated with infectious gastrointestinal disease in returned international travellers. J Infect 2009; 59: 19–27.
   PubMed Web of Science ®Google Scholar
• Stark D, Barratt JL, van Hal S, Marriott D, Harkness J, Ellis JT. Clinical significance of enteric protozoa in the immunosuppressed human population. Clin Microbiol Rev 2009; 22: 634–650.
   PubMed Web of Science ®Google Scholar